Small Ubiquitin-like Modifier (SUMO) Conjugation Impedes Transcriptional Silencing by the Polycomb Group Repressor Sex Comb on Midleg

Autor: Albert J. Courey, Matthew M. Smith, Jeffrey A. Simon, Daniel R. Mallin

Publikováno v: Journal of Biological Chemistry. 286:11391-11400

The Drosophila protein Sex Comb on Midleg (Scm) is a member of the Polycomb group (PcG), a set of transcriptional repressors that maintain silencing of homeotic genes during development. Recent findings have identified PcG proteins both as targets fo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b674677b9e617cc9e9f95210247f7033
https://doi.org/10.1074/jbc.m110.214569

Long-Range Repression by Multiple Polycomb Group (PcG) Proteins Targeted by Fusion to a Defined DNA-Binding Domain in Drosophila

Autor: Kelly Morgan, Pamela K. Geyer, Douglas Bornemann, Rachel Roberson, Timothy J. Parnell, Jeffrey A. Simon, Daniel R. Mallin, R.R. Roseman

Publikováno v: Genetics. 158:291-307

A tethering assay was developed to study the effects of Polycomb group (PcG) proteins on gene expression in vivo. This system employed the Su(Hw) DNA-binding domain (ZnF) to direct PcG proteins to transposons that carried the white and yellow reporte

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::938943c45fa6b9507566cc65e3f8987f
https://doi.org/10.1093/genetics/158.1.291

Comparative Analysis of Chromatin Binding by Sex Comb on Midleg (SCM) and Other Polycomb Group Repressors at a Drosophila Hox Gene▿

Autor: Ellen L. Miller, Neal Jahren, Daniel R. Mallin, Liangjun Wang, Jeffrey A. Simon, Carrie S. Ketel

Sex Comb on Midleg (SCM) is a transcriptional repressor in the Polycomb group (PcG), but its molecular role in PcG silencing is not known. Although SCM can interact with Polycomb repressive complex 1 (PRC1) in vitro, biochemical studies have indicate

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e59100642fae45113e504535a52047e
https://europepmc.org/articles/PMC2876513/

Unusual 2-aminopurine fluorescence from a complex of DNA and the EcoKI methyltransferase

Autor: R. Mallin, D. T. F. Dryden, C. Darlington, Bernard A. Connolly, Tsueu-Ju Su

Publikováno v: Su, T J, Connolly, B A, Darlington, C, Mallin, R & Dryden, D T F 2004, ' Unusual 2-aminopurine fluorescence from a complex of DNA and the EcoKI methyltransferase ', Nucleic Acids Research, vol. 32, no. 7, pp. 2223-2230 . https://doi.org/10.1093/nar/gkh531

The methyltransferase, M.EcoKI, recognizes the DNA sequence 5'-A (A) under bar CNNNNNNG (T) under bar TGC-3' and methylates adenine at the underlined positions. DNA methylation has been shown by crystallography to occur via a base flipping mechanism

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f2ae4d98e347914e3cf9f802b140964
https://europepmc.org/articles/PMC407817/

Polycomb group repression is blocked by the Drosophila suppressor of Hairy-wing [su(Hw)] insulator

Autor: J S Patton, J S Myung, Pamela K. Geyer, Daniel R. Mallin

Publikováno v: Genetics. 148(1)

The suppressor of Hairy-wing [SU(HW)] binding region disrupts communication between a large number of enhancers and promoters and protects transgenes from chromosomal position effects. These properties classify the SU(HW) binding region as an insulat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29703736a72a0d5a61a2213eb9a99618
https://pubmed.ncbi.nlm.nih.gov/9475743