Bovine Follicular Fluid Derived Extracellular Vesicles Modulate the Viability, Capacitation and Acrosome Reaction of Bull Spermatozoa

Autor: Mohammad Mehedi Hasan, Qurat Ul Ain Reshi, Freddy Lättekivi, Janeli Viil, Kasun Godakumara, Keerthie Dissanayake, Aneta Andronowska, Ülle Jaakma, Alireza Fazeli

Publikováno v: Biology, Vol 10, Iss 11, p 1154 (2021)

While follicular fluid (FF) is known to enhance the functional properties of spermatozoa, the role of FF-derived extracellular vesicles (EVs) in this respect is unknown. We hypothesized that bovine FF EVs convey signals to spermatozoa supporting sper

Externí odkaz: https://doaj.org/article/f75a1ba5f2b54966a3cdaec99201b083

Spermatozoa, acts as an external cue and alters the cargo and production of the extracellular vesicles derived from oviductal epithelial cells in vitro

Autor: Qurat Ul Ain Reshi, Kasun Godakumara, James Ord, Keerthie Dissanayake, Mohammad Mehedi Hasan, Aneta Andronowska, Paul Heath, Alireza Fazeli

Publikováno v: Reshi, Qurat Ul Ain; Godakumara, Kasun; Ord, James; Dissanayake, Keerthie; Hasan, Mohammad Mehedi; Andronowska, Aneta; Heath, Paul; Fazeli, Alireza (2022). Spermatozoa, acts as an external cue and alters the cargo and production of the extracellular vesicles derived from oviductal epithelial cells in vitro. (In Press). Journal of cell communication and signaling Springer 10.1007/s12079-022-00715-w

The oviduct provides optimum physiological and biochemical milieu essential for successful fertilization, early embryo development and facilitates functional maturation of spermatozoa. A study has revealed that spermatozoa alters the gene expression

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09832aa8e111e058d24ba194e2f363d0
https://doi.org/10.1007/s12079-022-00715-w

Characterization of Extracellular Vesicles Labelled with a Lipophilic Dye Using Fluorescence Nanoparticle Tracking Analysis

Autor: Keerthie Dissanayake, Toonika Rinken, Getnet Midekessa, Mohammad Mehedi Hasan, Alireza Fazeli, Qurat Ul Ain Reshi, Kasun Godakumara

Publikováno v: Membranes
Volume 11
Issue 10
Membranes, Vol 11, Iss 779, p 779 (2021)

Research on extracellular vesicles (EVs) has intensified over the past decade, including fluorescent membrane labeling of EVs. An optimal fluorescent method requires the size of EVs to be preserved after labeling. Lipophilic fluorescent dyes, such as

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::592633c392a965fd082a8e675d20120c
http://europepmc.org/articles/PMC8539200

Isolation of Extracellular Vesicles (EVs) Using Benchtop Size Exclusion Chromatography (SEC) Columns

Autor: Alireza Fazeli, Qurat Ul Ain Reshi, Keerthie Dissanayake, Mohammad Mehedi Hasan

Publikováno v: Methods in Molecular Biology ISBN: 9781071612453

A diverse group of lipid bilayer enclosed nanoparticles, referred to as extracellular vesicles (EVs), are released by all eukaryotic and prokaryotic cells into the extracellular space. The population of EVs being heterogeneous poses a challenge in th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a8832424006b70c8163d12e98cfb5b56
https://doi.org/10.1007/978-1-0716-1246-0_14

Oviduct as a sensor of embryo quality: deciphering the extracellular vesicle (EV)-mediated embryo-maternal dialogue

Autor: Andres Salumets, Qurat Ul Ain Reshi, Agne Velthut-Meikas, Yosra Ressaissi, James Ord, Janeli Viil, Ülle Jaakma, Monika Nõmm, Keerthie Dissanayake, Freddy Lättekivi, Kasun Godakumara, Kersti Jääger, Alireza Fazeli

Publikováno v: Journal of molecular medicine (Berlin, Germany). 99(5)

Embryo-derived extracellular vesicles (EVs) may play a role in mediating the embryo-maternal dialogue at the oviduct, potentially carrying signals reflecting embryo quality. We investigated the effects of bovine embryo-derived EVs on the gene express

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ae1ef6b42a41d504dedb09808e9cd89
https://pubmed.ncbi.nlm.nih.gov/33512581