Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Quentin R. V. Ferry"'
Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression
Autor:
David J. H. F. Knapp, Yale S. Michaels, Max Jamilly, Quentin R. V. Ferry, Hector Barbosa, Thomas A. Milne, Tudor A. Fulga
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
The utility of CRISPR-based technologies could be enhanced with the ability to control the spatial and temporal expression of gRNAs. Here the authors design a tRNA scaffold for highly specific gRNA production from a Pol II promoter.
Externí odkaz:
https://doaj.org/article/088592c408394975893eeb5eb3ce5546
Autor:
Qianxin Wu, Quentin R. V. Ferry, Toni A. Baeumler, Yale S. Michaels, Dimitrios M. Vitsios, Omer Habib, Roland Arnold, Xiaowei Jiang, Stefano Maio, Bruno R. Steinkraus, Marta Tapia, Paolo Piazza, Ni Xu, Georg A. Holländer, Thomas A. Milne, Jin-Soo Kim, Anton J. Enright, Andrew R. Bassett, Tudor A. Fulga
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-17 (2017)
RNA regulatory elements (RREs) are important post-transcriptional control features but studying them requires disrupting their activity without disturbing cellular homeostasis. Here the authors present GenERA, a CRISPR-Cas9 screening platform of in s
Externí odkaz:
https://doaj.org/article/db4eb21f42e2466d9fc7c9b4e4aa2f37
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-10 (2017)
Being able to regulate gene expression in a controllable manner is one of the promises of synthetic biology, and CRISPR-Cas9 is one of the tools being developed to achieve that goal. Here the authors develop an inducible CRISPR platform using an engi
Externí odkaz:
https://doaj.org/article/c3599b79b8d04d34aa8e0a984e7a35c2
Autor:
Quentin R. V. Ferry, Tudor A. Fulga
Publikováno v:
Methods in Molecular Biology ISBN: 9781071606865
The type-II CRISPR-Cas9 system has been repurposed to create synthetic programmable transcriptional regulators (CRISPR-TRs). Subsequent modifications of the system now allow for spatiotemporal control of CRISPR-mediated gene activation and repression
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::03199d167ee499146f7b9a76d48c1871
https://doi.org/10.1007/978-1-0716-0687-2_9
https://doi.org/10.1007/978-1-0716-0687-2_9
Autor:
Quentin R V, Ferry, Tudor A, Fulga
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2162
The type-II CRISPR-Cas9 system has been repurposed to create synthetic programmable transcriptional regulators (CRISPR-TRs). Subsequent modifications of the system now allow for spatiotemporal control of CRISPR-mediated gene activation and repression
Publikováno v:
Nature Communications
CRISPR-based transcription regulators (CRISPR-TRs) have transformed the current synthetic biology landscape by allowing specific activation or repression of any target gene. Here we report a modular and versatile framework enabling rapid implementati
Autor:
Christoffer Nellåker, Julia Steinberg, Chris P. Ponting, David R. FitzPatrick, Caleb Webber, Andrew Zisserman, Quentin R. V. Ferry
Publikováno v:
eLife, Vol 3 (2014)
eLife
eLife
Craniofacial characteristics are highly informative for clinical geneticists when diagnosing genetic diseases. As a first step towards the high-throughput diagnosis of ultra-rare developmental diseases we introduce an automatic approach that implemen
Autor:
Knapp, David J. H. F., Michaels, Yale S., Jamilly, Max, Ferry, Quentin R. V., Barbosa, Hector, Milne, Thomas A., Fulga, Tudor A.
Publikováno v:
Nature Communications; 4/2/2019, Vol. 10 Issue 1, pN.PAG-N.PAG, 1p