Zobrazeno 1 - 10
of 27
pro vyhledávání: '"QPNC-PAGE"'
Publikováno v:
Protocols in Semen Biology (Comparing Assays) ISBN: 9789811051999
The electrophoresis technique describes migration of charged particles under the influence of an electric field. The rate of migration depends upon various factors like charge of the particle, applied electric field, and temperature and nature of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b0045d7351b3ea2d153344fb8446d068
https://doi.org/10.1007/978-981-10-5200-2_18
https://doi.org/10.1007/978-981-10-5200-2_18
Publikováno v:
Analytical Letters. 41:1773-1784
Separation and quantification of molybdenum (Mo) in raw phloem sap from castor bean (Ricinus communis L.) was performed by size exclusion chromatography (SEC) and further purification was performed using quantitative preparative native continuous pol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b6082ea1a043ec4e6c97176ff4c17d9d
https://doi.org/10.1080/00032710802162442
https://doi.org/10.1080/00032710802162442
Publikováno v:
Analytical and Bioanalytical Chemistry. 389:1033-1045
One third of all genes of various organisms encode membrane proteins, emphasizing their crucial cellular role. However, due to their high hydrophobicity, membrane proteins demonstrate low solubility and a high tendency for aggregation. Indeed, conven
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ec95c7e1a90bae3163322d0977ee447
https://doi.org/10.1007/s00216-007-1514-6
https://doi.org/10.1007/s00216-007-1514-6
Autor:
G.M. Rothe
Polyacrylamide gradient gel electrophoresis in its native form can be used to separate water-soluble and detergent-soluble membrane proteins. Here, the corresponding extraction, separation, and visualization methods are described to gain membrane pro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ebab0df78da308596d0085fa52c6c0b7
https://doi.org/10.1016/b978-0-12-409547-2.11714-7
https://doi.org/10.1016/b978-0-12-409547-2.11714-7
Autor:
Bernd Kastenholz
Publikováno v:
Protein & Peptide Letters. 13:503-508
The quantitative analysis of metallochaperone proteins in biofluids (e.g. blood, liquor) may be a major prerequisite for clinical investigations concerning the structure-function relationships of biologically-active metal cofactor-containing chaperon
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ef823ada724c989f22271f76bc464d3
https://doi.org/10.2174/092986606776819637
https://doi.org/10.2174/092986606776819637
Autor:
Cristobal G. dos Remedios, Murat Kekic
Publikováno v:
Electrophoresis. 20:2053-2058
We describe how the interaction between actin and its protein ligands can be used to evaluate the presence of certain metal (Cd, Cu, Hg, Zn) ions and organic compounds (2,4-dioxin or Picloram) which are common components of environmental pollution. T
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3926e4d5de026b0265604a4bdc179828
https://doi.org/10.1002/(sici)1522-2683(19990701)20:10<2053::aid-elps2053>3.0.co
https://doi.org/10.1002/(sici)1522-2683(19990701)20:10<2053::aid-elps2053>3.0.co
Autor:
David E. Garfin, Bernd Kastenholz
Publikováno v:
Nature Precedings
Nature precedings (2010). doi:10.1038/npre.2010.4617.1
Nature precedings (2010). doi:10.1038/npre.2010.4617.1
A standard protocol for isolating metalloproteins in complex biological samples is presented using quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC-PAGE)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5662b82d2de9c98358d7f03f8eae4081
https://doi.org/10.1038/npre.2010.4617.1
https://doi.org/10.1038/npre.2010.4617.1
Publikováno v:
Analytical Biochemistry. 282:147-149
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::faf25beb13a358643f9ed4b381f3ad44
https://doi.org/10.1006/abio.2000.4598
https://doi.org/10.1006/abio.2000.4598
Autor:
Ana-Maria Sevcenco, Gerard C. Krijger, Wilfred R. Hagen, Peter-Leon Hagedoorn, Peter D. E. M. Verhaert, Martijn W. H. Pinkse
Publikováno v:
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry. 14(4)
A combination of techniques to separate and quantify the native proteins associated with a particular transition metal ion from a cellular system has been developed. The procedure involves four steps: (1) labeling of the target proteins with a suitab
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91a32bb8ce32887e717d21d231393a24
https://pubmed.ncbi.nlm.nih.gov/19205756
https://pubmed.ncbi.nlm.nih.gov/19205756
Autor:
Bernd Kastenholz
Publikováno v:
Nature Precedings
The Open Biochemistry Journal
The Open Biochemistry Journal
Currently, no pharmaceuticals for the etiological treatment of degenerative protein-misfolding diseases (e.g., ALS, Alzheimer’s or prion diseases) are commercially available. Therefore, in this technical note theoretical considerations and practica
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af21d1a02bfc68179d5beb19db8deefa
https://doi.org/10.1038/npre.2008.1431.2
https://doi.org/10.1038/npre.2008.1431.2