Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Pulakesh Aich"'
Autor:
Sampa Biswas, Pulakesh Aich
Publikováno v:
Cell Biochemistry and Biophysics. 76:219-229
Pro-domain of a cysteine cathepsin contains a highly conserved Ex2Rx2Fx2Nx3Ix3N (ERFNIN) motif. The zymogen structure of cathepsins revealed that the Arg(R) residue of the motif is a central residue of a salt-bridge/H-bond network, stabilizing the sc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::438d687ea2b504c7b0b04234ea7e0d85
https://doi.org/10.1007/s12013-017-0838-x
https://doi.org/10.1007/s12013-017-0838-x
Autor:
In-Chul Hwang, Rumum Rohman, Pulakesh Aich, Younghoon Kim, Kyung Lock Kim, Kimoon Kim, Joon Ho Roh, Soonsang Hong, Bahiru Punja Benke, Eun Hui Lee
Publikováno v:
Journal of the American Chemical Society. 139:7432-7435
We report here a synthetic ion channel developed from a shape-persistent porphyrin-based covalent organic cage. The cage was synthesized by employing a synthetically economical dynamic covalent chemistry (DCC) approach. The organic cage selectively t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d3bf1af0bc7751efb64c86a692a71e9
https://doi.org/10.1021/jacs.7b02708
https://doi.org/10.1021/jacs.7b02708
Autor:
Joon Ho Roh, James Brooke Murray, Junghyun Kim, Kimoon Kim, Pulakesh Aich, Jaeyeon An, Byeongseon Yang, Kyeng Min Park, Hyung Joon Cha, Young Ho Ko
Publikováno v:
Chemical communications (Cambridge, England). 54(89)
Here we report a recombinant protein (MS) obtained by genetic fusion of a mussel foot protein (Mfp3) motif into a silk spidroin (MaSp1). The MS not only self-assembled into a supramolecular fibre, as does the parent MaSp1, but also showed enhanced ad
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2c63340cc345ce7d221d9ce3c8599c1
https://pubmed.ncbi.nlm.nih.gov/30357157
https://pubmed.ncbi.nlm.nih.gov/30357157
Publikováno v:
The Protein Journal. 31:366-373
In the standard method of transformation of Escherichia coli with extraneous DNA, cells are made competent for DNA uptake by incubating in ice-cold 100 mM CaCl(2). Analysis of the whole protein profile of CaCl(2)-treated E. coli cells by the techniqu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b8376897ba320b29e43178733ad625f
https://doi.org/10.1007/s10930-012-9411-z
https://doi.org/10.1007/s10930-012-9411-z
Publikováno v:
Biopolymers. 89:606-613
With increasing addition of Escherichia coli LPS to calf thymus DNA, both dissolved in CaCl2, absorption maxima of DNA at 260 nm decreased gradually with the appearance of isosbastic points at both ends of spectra, which implied some binding between
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10b82827742123bc6047583986beb11a
https://doi.org/10.1002/bip.20964
https://doi.org/10.1002/bip.20964
Publikováno v:
Molecular Membrane Biology. 25:411-422
Artificial transformation of Escherichia coli with plasmid DNA in presence of CaCl2 is a widely used technique in recombinant DNA technology. However, exact mechanism of DNA transfer across cell membranes is largely obscure. In this study, measuremen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::950eedcdca2d9791936079935fd54fdf
https://doi.org/10.1080/09687680802187765
https://doi.org/10.1080/09687680802187765
Publikováno v:
Soft matter. 11(45)
Electrostatic interactions of RNA are at the center of determining the dynamical flexibility and structural stability. By analysing neutron scattering spectroscopy, we show that fast dynamics of hydrated tRNA on ps to ns timescales increases with str
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6aebf75a0d876672f350e9791ef2846f
https://pubmed.ncbi.nlm.nih.gov/26430908
https://pubmed.ncbi.nlm.nih.gov/26430908
Publikováno v:
Acta crystallographica. Section D, Biological crystallography. 68(Pt 12)
Papain is the archetype of a broad class of cysteine proteases (clan C1A) that contain a pro-peptide in the zymogen form which is required for correct folding and spatio-temporal regulation of proteolytic activity in the initial stages after expressi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5815dc01be144f2cf0ae0a2dd33559c8
https://pubmed.ncbi.nlm.nih.gov/23151624
https://pubmed.ncbi.nlm.nih.gov/23151624
Publikováno v:
Biomacromolecules. 9(9)
In the standard procedure for artificial transformation of E. coli by plasmid DNA, cellular competence for DNA uptake is developed by suspending the cells in ice-cold CaCl2 (50-100 mM). It is believed that CaCl2 helps DNA adsorption to the lipopolysa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3d5887faac8b32ce93e47033c54f206
https://pubmed.ncbi.nlm.nih.gov/18698848
https://pubmed.ncbi.nlm.nih.gov/18698848
Autor:
Rakhi Dasgupta, Monobesh Patra, Sourav Roy, Pulakesh Aich, Arijit Kumar Chatterjee, Tarakdas Basu, Syed Asrafuzzaman
Publikováno v:
Biophysical Journal. 100(3):209a-210a
IbpA and IbpB (inclusion body binding proteins A and B) are two small heat shock proteins in E. coli. Earlier studies report that IbpB has holdase property by which it binds denatured, aggregate-prone proteins forming stable complexes, but has no fol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78b514f8f319c44d8f25f8a348eb3997