Výsledky vyhledávání - "Proteins/analysis/*chemistry"

Two-dimensional gel electrophoresis for proteome projects: The effects of protein hydrophobicity and copy number

Autor: Elisabeth Gasteiger, Marc R. Wilkins, Denis F. Hochstrasser, Jean-Charles Sanchez, Amos Marc Bairoch

Publikováno v: Electrophoresis, Vol. 19, No 8-9 (1998) pp. 1501-5

Two-dimensional (2-D) gel electrophoresis is often used in proteome projects to provide a global view of the proteins expressed in any cell or tissue type. Here we have investigated the effects of protein hydrophobicity and cellular protein copy numb

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d15d1290ecc6270c54d59eef126d0e7e
https://doi.org/10.1002/elps.1150190847

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A Molecular Scanner To Automate Proteomic Research and To Display Proteome Images

Publikováno v: Analytical Chemistry, Vol. 71, No 21 (1999) pp. 4981-8

Identification and characterization of all proteins expressed by a genome in biological samples represent major challenges in proteomics. Today's commonly used high-throughput approaches combine two-dimensional electrophoresis (2-DE) with peptide mas

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30a3ec1c8925f550682a32ecb01e3f18
https://doi.org/10.1021/ac990449e

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Qualitative and quantitative analysis of glycated proteins in human plasma by glucose isotopic labeling with ¹³C6-reducing sugars

Autor: Priego-Capote Feliciano, Ramírez-Boo Maria, Hochstrasser Denis, Sanchez Jean-Charles

Publikováno v: Methods in Molecular Biology, Vol. 728 (2011) pp. 219-32
Methods in molecular biology (Clifton N.J.)

Glucose is the predominant source of energy in cells. However, a chronic high glucose exposure of proteins modifies a number of biological pathways, known as glucotoxicity. Several studies have suggested that this impaired protein function is associa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::7499b9242bdb6fb449ad089e590e3971
https://pubmed.ncbi.nlm.nih.gov/21468951

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Fast uncoiling kinetics of F1C pili expressed by uropathogenic Escherichia coli are revealed on a single pilus level using force-measuring optical tweezers

Autor: Bernt Eric Uhlin, Stina Lindberg, Sarah Ehlers, Mickaël Castelain, Jeanna Klinth, Magnus Andersson, Ove Axner

Publikováno v: European Biophysics Journal
European Biophysics Journal, Springer Verlag (Germany), 2011, 40 (3), pp.305-316. ⟨10.1007/s00249-010-0648-1⟩

International audience; Uropathogenic Escherichia coli (UPEC) express various kinds of organelles, so-called pili or fimbriae, that mediate adhesion to host tissue in the urinary tract through specific receptor-adhesin interactions. The biomechanical

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64fc3f188db29f381260e0c54591ba98
https://hal.inrae.fr/hal-02644269

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Plasma and red blood cell protein maps: Update 1993

Autor: Jean-Charles Sanchez, Luc P. Balant, Nicole Paquet, Denis F. Hochstrasser, Séverine Frutiger, Olivier Georges Golaz, Claude Walzer, Graham J. Hughes, Amos Marc Bairoch, Christian Pasquali, Jean-Daniel Tissot, Ron D. Appel

Publikováno v: Electrophoresis, Vol. 14, No 11 (1993) pp. 1223-31

This publication updates the reference plasma and red blood cell protein maps obtained with immobilized pH gradients. Seventeen polypeptide spots or chains were partially characterized by direct N-terminal sequencing or by sequencing of peptides obta

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::41eb6aedf61cfeb865556dfa831a089f
https://doi.org/10.1002/elps.11501401183

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Combining low- and high-energy tandem mass spectra for optimized peptide quantification with isobaric tags

Autor: Jean-Charles Sanchez, Loïc Dayon, Christine Hoogland, Alexander Scherl, Carla Pasquarello

Publikováno v: Journal of Proteomics, Vol. 73, No 4 (2010) pp. 769-777

Isobaric tagging, via TMT or iTRAQ, is widely used in quantitative proteomics. To date, tandem mass spectrometric analysis of isobarically-labeled peptides with hybrid ion trap-orbitrap (LTQ-OT) instruments has been mainly carried out with higher-ene

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fad0c2036da1baf1b00f8d37a176699
https://archive-ouverte.unige.ch/unige:20874

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Complete genomic characterization of an intertypic Sabin 3/Sabin 2 capsid recombinant

Autor: Zissis Mamuris, Christos Brakoulias, Panayotis Markoulatos, Vaia Pliaka, Stamatina Levidiotou-Stefanou, Evaggelos Dedepsidis, Anastassia Pratti, Z. Kyriakopoulou

The genetic properties of strain K/2002, isolated from fecal samples of a 7-month-old child who had received his first oral poliovirus vaccine (OPV) dose at the age of 3 months, are described. Preliminary sequencing characterization of isolate K/2002

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0795e80a81fea192cfc652a4bc3415a0
http://olympias.lib.uoi.gr/jspui/handle/123456789/22879

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Gold coating of non-conductive membranes before matrix-assisted laser desorption/ionization tandem mass spectrometric analysis prevents charging effect

Autor: Alexander, Scherl, Catherine G, Zimmermann-Ivol, Joel, Di Dio, Ali R, Vaezzadeh, Pierre-Alain, Binz, Michel, Amez-Droz, Roland, Cochard, Jean-Charles, Sanchez, Matthias, Glückmann, Denis F, Hochstrasser

Publikováno v: Rapid Communications in Mass Spectrometry, Vol. 19, No 5 (2005) pp. 605-10

Acquisition of tandem mass spectra from peptides or other analytes deposited on non-conductive membranes is inhibited on instruments combining matrix-assisted laser desorption/ionization with tandem time-of-flight analyzers (MALDI-TOF/TOF) due to a c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::030d9a4afa7e81b311fc9f8c005fe0bc
https://archive-ouverte.unige.ch/unige:33517

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The molecular scanner: concept and developments

Autor: Carla Pasquarello, Garry L. Corthals, Christine Hoogland, Markus Müller, Ron D. Appel, Catherine Gabrielle Zimmermann, Pierre-Alain Binz, Denis F. Hochstrasser, Jean-Charles Sanchez

Publikováno v: Current Opinion in Biotechnology, Vol. 15, No 1 (2004) pp. 17-23

Approaches aimed at deciphering the proteome have illustrated the need for relatively complex and highly sensitive methodologies. The major elements of proteome analysis, such as powerful protein separation and enzymatic processing, mass spectrometry

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ffd89c65bc168951567b815afc811878
https://pubmed.ncbi.nlm.nih.gov/15102461

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N-t-butyliodoacetamide and iodoacetanilide: two new cysteine alkylating reagents for relative quantitation of proteins

Autor: Jean-Charles Sanchez, Denis F. Hochstrasser, Garry L. Corthals, Carla Pasquarello

Publikováno v: Rapid Communications in Mass Spectrometry, Vol. 18, No 1 (2004) pp. 117-27

The synthesis and application of two new alkylating reagents, N-tert-butyl-2-iodoacetamide (N-t-butyliodoacetamide) and 2-iodo-N-phenylacetamide (iodoacetanilide), are described. N-t-Butyliodoacetamide and iodoacetanilide were synthesised to purity i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2df980f6fafb2d3ca06b1b72ab876f26
https://pubmed.ncbi.nlm.nih.gov/14689568

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