Výsledky vyhledávání - "Protein combining"

Split-enzyme immunoassay to monitor EGFR-HER2 heterodimerization on cell surfaces

Autor: Sun Jin Kim, Shawn C. Owen, Andrew S. Dixon

Publikováno v: Acta Biomaterialia. 135:225-233

Over 30,000 protein-protein interactions with pathological implications have been identified; yet, discovering and investigating drugs that target these specific interactions is greatly limited by the inability to monitor native protein-protein inter

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81f589f1b839182692e5e05a573cd52c
https://doi.org/10.1016/j.actbio.2021.08.055

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Measuring the subcellular compartmentalization of viral infections by protein complementation assay

Autor: Sébastien Nisole, Virginie Georget, Cedric Hassen-Khodja, Yves Jacob, Yves L. Janin, Nathalie J. Arhel, Pierre-Olivier Vidalain, Thierry Rose, Juliette Fernandez

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2021, 118 (2), pp.e2010524118. ⟨10.1073/pnas.2010524118⟩
Proceedings of the National Academy of Sciences of the United States of America, 2021, 118 (2), pp.e2010524118. ⟨10.1073/pnas.2010524118⟩
Proc Natl Acad Sci U S A

International audience; The recent emergence and reemergence of viruses in the human population has highlighted the need to develop broader panels of therapeutic molecules. High-throughput screening assays opening access to untargeted steps of the vi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::41a471b2591482ca117d2433903462d2
https://hal.archives-ouvertes.fr/hal-03108728

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Molecular Imaging of Protein–Protein Interactions and Protein Folding

Autor: Ramasamy Paulmurugan, Tarik F. Massoud

In the last 2 decades, there has been significant interest in the field of reporter gene imaging, with the aim of determining the location(s), duration, and extent of gene expression within living subjects. An important application of this is in mole

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::147d487df81a1ecf2e254397792e8a6e
https://doi.org/10.1016/b978-0-12-816386-3.00071-5

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Proteomic Analysis Uncovers Measles Virus Protein C Interaction with p65/iASPP/p53 Protein Complex

Autor: Hyeju Eun, Mariette Matondo, Thibaut Douché, Regis Grailhe, Frédéric Tangy, Anastassia V. Komarova, Yves Jacob, Quentin Giai Gianetto, Marc Santolini, István Kovács, Chantal Combredet, Alice Meignié

Viruses manipulate central machineries of host cells to their advantage. They prevent host cell antiviral responses to create a favorable environment for their survival and propagation. Measles virus (MV) encodes two non-structural proteins MV-V and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0920a6350cbac36ad47fbcb44f4546e1

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X-ray Structure of the Human Karyopherin RanBP5, an Essential Factor for Influenza Polymerase Nuclear Trafficking

Autor: Thibaut Crépin, Bruno Da Costa, Christopher Swale, Imre Berger, Andrew A. McCarthy, Frederic Garzoni, Bernard Delmas, Rob W. H. Ruigrok, Laura Sedano, Christoph Bieniossek

Publikováno v: Journal of Molecular Biology
Journal of Molecular Biology, 2020, 432 (10), pp.3353-3359. ⟨10.1016/j.jmb.2020.03.021⟩
Journal of Molecular Biology, Elsevier, 2020, 432 (10), pp.3353-3359. ⟨10.1016/j.jmb.2020.03.021⟩
Journal of Molecular Biology, Elsevier, 2020, S0022-2836(20)30254-0, ⟨10.1016/j.jmb.2020.03.021⟩

International audience; Here, we describe the crystal structures of two distinct isoforms of ligand-free human karyopherin RanBP5 and investigate its global propensity to interact with influenza A virus polymerase. Our results confirm the general arc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33e8fdd993bac3b11d03f5437a199148
https://hal.univ-grenoble-alpes.fr/hal-02555361/document

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A Split Transcriptional Repressor That Links Protein Solubility to an Orthogonal Genetic Circuit

Autor: Emily E. Thomas, Laura Segatori, Yimeng Zeng, Alicia M. Jones, Jonathan J. Silberg, Barbara Nassif

Publikováno v: ACS Synthetic Biology. 7:2126-2138

Monitoring the aggregation of proteins within the cellular environment is key to investigating the molecular mechanisms underlying the formation of off-pathway protein assemblies associated with the development of disease and testing therapeutic stra

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cff4144f8d159c993044a006cccc900d
https://doi.org/10.1021/acssynbio.8b00129

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Cell-based, bioluminescent assay for monitoring the interaction between PCSK9 and the LDL receptor

Autor: James J. Cali, Sarah Duellman, Jolanta Vidugiriene, Thomas Machleidt

Publikováno v: Journal of Lipid Research
Journal of Lipid Research, Vol 58, Iss 8, Pp 1722-1729 (2017)

Monitoring the expression of cell-surface receptors, their interaction with extracellular ligands, and their fate upon ligand binding is important for understanding receptor function and developing new therapies. We describe a cell-based method that

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::106bc9d384c2ea5494978d0ef86e9339
https://doi.org/10.1194/jlr.d074658

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A Tri-part Protein Complementation System Using Antibody-Small Peptide Fusions Enables Homogeneous Immunoassays

Autor: Sun Jin Kim, Sylvia Krippner, Andrew S. Dixon, Brett K. Baumgartner, Shawn C. Owen

Publikováno v: Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
Scientific Reports

Protein-fragment complementation is a valuable tool for monitoring protein interactions. In complementation assays, the reporter fragments are directly fused to the interacting proteins, eliminating the possibility of monitoring native interactions.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::326ad08582b7c7e9542f2305a9efb7fd
https://doaj.org/article/97ac8ae7398d48fb8a4f94d4d926ddba

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Development of a bimolecular luminescence complementation assay for RGS: G protein interactions in cells

Autor: David L. Roman, Michael P. Hayes, Christopher Bodle, Joseph B. O’Brien

Publikováno v: Analytical Biochemistry. 522:10-17

Cell based assessment tools and screening platforms are the preferred paradigm for small molecule identification and validation due to selectively identifying molecules with cellular activity and validation of compound activity against target protein

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::719f7370ce5b8195c80009f7cc843a02
https://doi.org/10.1016/j.ab.2017.01.013

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