Zobrazeno 1 - 10
of 1 414
pro vyhledávání: '"Protein biochemistry"'
Autor:
Li Huang, Zheng Zhou
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103295- (2024)
Summary: The histone lysine methyltransferase SUV420H1 preferentially targets the H2A.Z-containing nucleosome core particle (H2A.Z-NCP) and catalyzes the H4K20me2 modification at replication origins. Here, we present a protocol for preparing SUV420H1
Externí odkaz:
https://doaj.org/article/c0419efcd6d6408eb62d9920c08ec4ab
Autor:
Javier Negueruela, Valerie Vandenbempt, Stephanie Talamantes, Francisco Ribeiro-Costa, Mariana Nunes, André Dias, Mayank Bansal, Esteban N. Gurzov
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103297- (2024)
Summary: Gene editing of human pluripotent stem cells is a promising approach for developing targeted gene therapies for metabolic diseases. Here, we present a protocol for generating a CRISPR-Cas12a gene knockout of protein tyrosine phosphatases in
Externí odkaz:
https://doaj.org/article/c8a881473ccd4570afc4e34c60812a51
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103275- (2024)
Summary: Heat shock (HS) coincides with the assembly of translationally arrested heat shock messenger ribonucleoprotein particles (HS-mRNPs) and condensates. Here, we present a protocol to reconstitute HS-mRNPs and HS condensates with eIF4G, eIF4E, P
Externí odkaz:
https://doaj.org/article/b111396c447f4359803cc261fe35a690
Autor:
Pablo Martinez, Henika Patel, Yanwen You, Emma H. Doud, Amber L. Mosley, Cristian A. Lasagna-Reeves
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103185- (2024)
Summary: The aggregation and spreading of “tau-seeds” are key for the development and progression of tauopathies, including Alzheimer’s disease. Here we describe the steps to isolate and analyze biochemically active tau-seeds from human, mouse,
Externí odkaz:
https://doaj.org/article/9aab8bca630c4876a186cab5b1948ee3
Autor:
Jin Li, Chao Liang
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103220- (2024)
Summary: AS1411-NCL-MDM2-based proteolysis-targeting chimeras (ANM-PROTACs) are capable of inducing selective degradation of transcription factors (TFs) in tumor cells. Here, we present a protocol for constructing ANM-PROTACs. We describe steps for m
Externí odkaz:
https://doaj.org/article/3b5fc8a868614e8bb7d43cdfe046198e
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103171- (2024)
Summary: Here, we present a protocol to quantify interactions among difficult-to-express proteins from Drosophila cells using the select western blot-free tagged-protein interaction (SWFTI) assay. We describe steps for plasmid design, cell plating, p
Externí odkaz:
https://doaj.org/article/63f979aa131f4ea3a13b457ed2186e02
Autor:
Prashant Kaushal, Manisha R. Ummadi, Gwendolyn M. Jang, Yennifer Delgado, Sara K. Makanani, Kareem Alba, Declan M. Winters, Sophie F. Blanc, Jiewei Xu, Benjamin Polacco, Yuan Zhou, Erica Stevenson, Manon Eckhardt, Lorena Zuliani-Alvarez, Robyn Kaake, Danielle L. Swaney, Nevan J. Krogan, Mehdi Bouhaddou
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103286- (2024)
Summary: Proteins congregate into complexes to perform diverse cellular functions. Protein complexes are remodeled by protein-coding mutations or cellular signaling changes, driving phenotypic outcomes in health and disease. We present an affinity pu
Externí odkaz:
https://doaj.org/article/612d42808f5c41db95668238fe6e55eb
Autor:
Elaheh Jalali, Javad Sargolzaei
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103429- (2024)
Summary: Investigating the interactions between ligands, such as drugs, and proteins is essential to understand their functions and effects. This fluorescence spectroscopy protocol details how to analyze the effects of drugs on protein structure, inc
Externí odkaz:
https://doaj.org/article/f92f567091e14c51a33239ead10cc905
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103367- (2024)
Summary: The search and development of new quorum-sensing (QS) inhibitors are ongoing processes for biofilm control. Here, we present a protocol for in silico characterization of natural-based molecules as QS inhibitors. We describe steps for prepari
Externí odkaz:
https://doaj.org/article/1741bffa5d3149c6a02c093a5e8a7925
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103328- (2024)
Summary: Here, we present a lysate-based split-luciferase assay for examining protein-protein interactions (PPIs) in HEK293T cell lysates, exemplified by interactions between subunits of protein phosphatase PP1. We describe steps for storing and re-u
Externí odkaz:
https://doaj.org/article/fad32e190db7479dbfe6512f204f0542