A protocol for measuring the activity of protein kinase C-delta in murine bone-marrow-derived dendritic cells.

Autor: Mohati SM; Dalhousie University, Department of Microbiology and Immunology, Halifax, NS B3K 6R8, Canada., Matak AM; Dalhousie University, Department of Microbiology and Immunology, Halifax, NS B3K 6R8, Canada., Makdissi S; Dalhousie University, Department of Microbiology and Immunology, Halifax, NS B3K 6R8, Canada., Di Cara F; Dalhousie University, Department of Microbiology and Immunology, Halifax, NS B3K 6R8, Canada. Electronic address: dicara@dal.ca.

Publikováno v: STAR protocols [STAR Protoc] 2024 Sep 20; Vol. 5 (3), pp. 103208. Date of Electronic Publication: 2024 Jul 26.

Protein kinase C delta regulates mononuclear phagocytes and hinders response to immunotherapy in cancer.

Autor: Chaib M; Department of Pharmaceutical Sciences, College of Pharmacy, The University of Tennessee Health Science Center, Memphis, TN 38163, USA., Holt JR; Department of Medicine, Division of Hematology and Oncology, College of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USA., Fisher EL; Vanderbilt Center for Immunobiology and Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37235, USA., Sipe LM; Department of Medicine, Division of Hematology and Oncology, College of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USA., Bohm MS; Department of Microbiology, Immunology, and Biochemistry, College of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USA., Joseph SC; Department of Medicine, Division of Hematology and Oncology, College of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USA., Simmons BW; Department of Medicine, Division of Hematology and Oncology, College of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USA., Eugin Simon S; Department of Medicine, Division of Hematology and Oncology, College of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USA., Yarbro JR; Department of Medicine, Division of Hematology and Oncology, College of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USA., Tanveer U; Department of Medicine, Division of Hematology and Oncology, College of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USA., Halle JL; Department of Physical Therapy, College of Health Professions, The University of Tennessee Health Science Center, Memphis, TN 38163, USA., Carson JA; Department of Physical Therapy, College of Health Professions, The University of Tennessee Health Science Center, Memphis, TN 38163, USA., Hollingsworth TJ; Department of Microbiology, Immunology, and Biochemistry, College of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USA.; Department of Ophthalmology, Hamilton Eye Institute, College of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USA.; Department of Anatomy and Neurobiology, College of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USA., Wei Q; Department of Cellular Biology and Anatomy, Augusta University, Augusta, GA 30912, USA., Rathmell JC; Vanderbilt Center for Immunobiology and Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37235, USA., Thomas PG; Department of Microbiology, Immunology, and Biochemistry, College of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USA.; Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA., Hayes DN; Department of Medicine, Division of Hematology and Oncology, College of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USA.; UTHSC Center for Cancer Research, College of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USA., Makowski L; Department of Pharmaceutical Sciences, College of Pharmacy, The University of Tennessee Health Science Center, Memphis, TN 38163, USA.; Department of Medicine, Division of Hematology and Oncology, College of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USA.; Department of Microbiology, Immunology, and Biochemistry, College of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USA.; UTHSC Center for Cancer Research, College of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USA.

Publikováno v: Science advances [Sci Adv] 2023 Dec 22; Vol. 9 (51), pp. eadd3231. Date of Electronic Publication: 2023 Dec 22.

Protein kinase C delta enhances the diagnostic performance of hepatocellular carcinoma.

Autor: Nakagawa C; Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan., Oikawa T; Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan., Yamada K; Department of Biochemistry, The Jikei University School of Medicine, Tokyo, Japan., Tsubota A; Project Research Units, Research Center for Medical Science, The Jikei University School of Medicine, Tokyo, Japan., Saeki C; Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan., Katagiri K; Department of Biochemistry, The Jikei University School of Medicine, Tokyo, Japan., Tago N; Department of Biochemistry, The Jikei University School of Medicine, Tokyo, Japan., Kamioka H; Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan.; Department of Biochemistry, The Jikei University School of Medicine, Tokyo, Japan., Ueda K; Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan., Haruki K; Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The Jikei University School of Medicine, Tokyo, Japan., Furukawa K; Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The Jikei University School of Medicine, Tokyo, Japan., Nakano M; Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan., Torisu Y; Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan., Ikegami T; Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The Jikei University School of Medicine, Tokyo, Japan., Yoshida K; Department of Biochemistry, The Jikei University School of Medicine, Tokyo, Japan., Saruta M; Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan.

Publikováno v: Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals [Biomarkers] 2024 Mar; Vol. 29 (2), pp. 55-67. Date of Electronic Publication: 2024 Feb 15.

A protocol for measuring the activity of protein kinase C-delta in murine bone-marrow-derived dendritic cells

Autor: Seyedeh Mahdiye Mohati, Arash Mohammadi Matak, Stephanie Makdissi, Francesca Di Cara

Publikováno v: STAR Protocols, Vol 5, Iss 3, Pp 103208- (2024)

Summary: Protein kinase C-δ (PKC-δ) is a key enzyme controlling growth, differentiation, and apoptosis in various cells, including immune cells. Here, we present a protocol to determine PKC-δ activation in response to increased membrane-bound diac

Externí odkaz: https://doaj.org/article/b627674bb4454d0da0c2ed8adaab8935

Proteomic profiling reveals CEACAM6 function in driving gallbladder cancer aggressiveness through integrin receptor, PRKCD and AKT/ERK signaling.

Autor: Sugiyanto RN; Institute of Pathology, University Hospital Heidelberg, Heidelberg University, Heidelberg, Germany., Metzger C; Institute of Pathology, University Hospital Heidelberg, Heidelberg University, Heidelberg, Germany., Inal A; Institute of Pathology, University Hospital Heidelberg, Heidelberg University, Heidelberg, Germany., Truckenmueller F; Institute of Pathology, University Hospital Heidelberg, Heidelberg University, Heidelberg, Germany., Gür K; Institute of Pathology, University Hospital Heidelberg, Heidelberg University, Heidelberg, Germany., Eiteneuer E; Institute of Pathology, University Hospital Heidelberg, Heidelberg University, Heidelberg, Germany., Huth T; Institute of Pathology, University Hospital Heidelberg, Heidelberg University, Heidelberg, Germany., Fraas A; Institute of Pathology, University Hospital Heidelberg, Heidelberg University, Heidelberg, Germany., Heinze I; Leibniz Institute on Aging-Fritz Lipmann Institute (FLI), Jena, Germany., Kirkpatrick J; Leibniz Institute on Aging-Fritz Lipmann Institute (FLI), Jena, Germany., Sticht C; NGS Core Facility, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany., Albrecht T; Institute of Pathology, University Hospital Heidelberg, Heidelberg University, Heidelberg, Germany.; Liver Cancer Centre Heidelberg (LCCH), Heidelberg, Germany., Goeppert B; Institute of Pathology, University Hospital Heidelberg, Heidelberg University, Heidelberg, Germany.; Institute of Tissue Medicine and Pathology, University of Bern, Bern, Switzerland.; Institute of Pathology and Neuropathology, RKH Hospital Ludwigsburg, Ludwigsburg, Germany., Poth T; Center for Model System and Comparative Pathology, Institute of Pathology, University Hospital Heidelberg, Heidelberg University, Heidelberg, Germany., Pusch S; Clinical Cooperation Unit Neuropathology, German Cancer Research Center (DKFZ), Heidelberg, Germany.; Department of Neuropathology, Institute of Pathology, University Hospital Heidelberg, Heidelberg, Germany., Mehrabi A; Liver Cancer Centre Heidelberg (LCCH), Heidelberg, Germany.; Department of General Visceral and Transplantation Surgery, University Hospital Heidelberg, Heidelberg, Germany., Schirmacher P; Institute of Pathology, University Hospital Heidelberg, Heidelberg University, Heidelberg, Germany.; Liver Cancer Centre Heidelberg (LCCH), Heidelberg, Germany., Ji J; The MOE Key Laboratory of Biosystems Homeostasis & Protection, Zhejiang Provincial Key Laboratory for Cancer Molecular Cell Biology, Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang, China., Ori A; Leibniz Institute on Aging-Fritz Lipmann Institute (FLI), Jena, Germany., Roessler S; Institute of Pathology, University Hospital Heidelberg, Heidelberg University, Heidelberg, Germany. Stephanie.Roessler@med.uni-heidelberg.de.; Liver Cancer Centre Heidelberg (LCCH), Heidelberg, Germany. Stephanie.Roessler@med.uni-heidelberg.de.

Publikováno v: Cell death & disease [Cell Death Dis] 2024 Oct 28; Vol. 15 (10), pp. 780. Date of Electronic Publication: 2024 Oct 28.