Výsledky vyhledávání

Akademický článek

Laminin 521 maintains differentiation potential of mouse and human satellite cell-derived myoblasts during long-term culture expansion

Autor: Penton, Christopher M., Badarinarayana, Vasudeo, Prisco, Joy, Powers, Elaine, Pincus, Mark, Allen, Ronald E., August, Paul R.

Background: Large-scale expansion of myogenic progenitors is necessary to support the development of high-throughput cellular assays in vitro and to advance genetic engineering approaches necessary to develop cellular therapies for rare muscle diseas

Externí odkaz: http://hdl.handle.net/10150/622727
http://arizona.openrepository.com/arizona/handle/10150/622727

Zobrazit plný text záznamu

Akademický článek

Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
K zobrazení výsledku je třeba se přihlásit.

Additional file 3: Figure S3. of Laminin 521 maintains differentiation potential of mouse and human satellite cell-derived myoblasts during long-term culture expansion

Autor: Penton, Christopher, Vasudeo Badarinarayana, Prisco, Joy, Powers, Elaine, Pincus, Mark, Allen, Ronald, August, Paul

Laminin 521 promotes increased human myogenic cell proliferation. Cells were expanded on laminin 111, laminin 211, laminin 521, or growth factor reduced Matrigel for 5 passages. For proliferation analysis, cells were seeded at a density of approximat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1811300e859c0c40de51a9764c1dff71

Zobrazit plný text záznamu

Additional file 4: Figure S4. of Laminin 521 maintains differentiation potential of mouse and human satellite cell-derived myoblasts during long-term culture expansion

Autor: Penton, Christopher, Vasudeo Badarinarayana, Prisco, Joy, Powers, Elaine, Pincus, Mark, Allen, Ronald, August, Paul

Laminin 521 promotes increased mdx/BL10 differentiation. Cells were freshly isolated from male mdx/BL10 limb muscles and plated on multiple matrices: laminin 111, laminin 211, laminin 521, and growth factor reduced Matrigel. MHC area quantification s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1fa768ec37fce746fdaca953611d2815

Zobrazit plný text záznamu

Additional file 2: Figure S2. of Laminin 521 maintains differentiation potential of mouse and human satellite cell-derived myoblasts during long-term culture expansion

Autor: Penton, Christopher, Vasudeo Badarinarayana, Prisco, Joy, Powers, Elaine, Pincus, Mark, Allen, Ronald, August, Paul

FACS sort of DBA/2J satellite cells. Cells were sorted for granularity (SSC × FSC), singlet selection (FSC-W × FSC-H, SSC-W × SSC-H), live/dead (propidium iodide), CD31−/CD45− (Percp-eFluor710), Pdgfrα−/Sca1− (BV421/BV605), and integrin �

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::288ec72411e6ee9bf08d0862186877ca

Zobrazit plný text záznamu

Additional file 6: Figure S6. of Laminin 521 maintains differentiation potential of mouse and human satellite cell-derived myoblasts during long-term culture expansion

Autor: Penton, Christopher, Vasudeo Badarinarayana, Prisco, Joy, Powers, Elaine, Pincus, Mark, Allen, Ronald, August, Paul

Cell counts for freshly isolated and expanded myoblasts reveal similar cell adherence across all matrices. (A) Cell counts from freshly isolated Dba, BL6, and BL10 cells 5 days post isolation show similar cell adherence on all matrices. (B) Cell coun

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0bdc3139c777e66c54104eed912f9e50

Zobrazit plný text záznamu

Additional file 5: Figure S5. of Laminin 521 maintains differentiation potential of mouse and human satellite cell-derived myoblasts during long-term culture expansion

Autor: Penton, Christopher, Vasudeo Badarinarayana, Prisco, Joy, Powers, Elaine, Pincus, Mark, Allen, Ronald, August, Paul

Quantification of protein coating for various ECM proteins. Laminin 111, laminin 211, laminin 521, and gelatin coat similarly, while Matrigel is coated at a higher amount. Nâ =â 12 technical replicates. Data is presented as mean with error bars rep

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ba813bf4be319aa5a9465f36bd390a20

Zobrazit plný text záznamu

Additional file 1: Figure S1. of Laminin 521 maintains differentiation potential of mouse and human satellite cell-derived myoblasts during long-term culture expansion

Autor: Penton, Christopher, Vasudeo Badarinarayana, Prisco, Joy, Powers, Elaine, Pincus, Mark, Allen, Ronald, August, Paul

Laminin chain structure. Laminins are heterotrimeric complexes annotated in the order of α, β, and λ chains. Examples are laminin 111 = α1, β1, and λ1 and laminin 521 = α5, β2, and λ1.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24c4cbe9aec347ef7fcf4fdc1374b269

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání