Zobrazeno 1 - 10
of 1 301
pro vyhledávání: '"Primer dimer"'
Publikováno v:
BioTechniques, Vol 61, Iss 6, Pp 293-296 (2016)
Externí odkaz:
https://doaj.org/article/79374348a63849ebbcfaa2bb5b568334
Autor:
Marina Mokhtar et.al
Publikováno v:
Turkish Journal of Computer and Mathematics Education (TURCOMAT). 12:1011-1017
Quantitative real-time polymerase chain reaction (qRT-PCR) is one of the most common methods for gene expression studies. Data normalization based on reference genes is essential for qRT-PCR assays. This study identifies suitable reference genes for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3f7dd3f743956babe6f61ba435c95a1c
https://doi.org/10.17762/turcomat.v12i3.837
https://doi.org/10.17762/turcomat.v12i3.837
Autor:
Aman Russom, Ruben R. G. Soares, Xiushan Yin, Ahmad Saleem Akhtar, Noa Lapins, Donal Barrett, Vicent Pelechano, Gustaf Sandh, Inês F. Pinto
Publikováno v:
Lab on a Chip. 21:2932-2944
With its origin estimated around December 2019 in Wuhan, China, the ongoing SARS-CoV-2 pandemic is a major global health challenge. The demand for scalable, rapid and sensitive viral diagnostics is thus particularly pressing at present to help contai
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45d0d1e06056a6e29e623c2d778aaf55
https://doi.org/10.1039/d1lc00266j
https://doi.org/10.1039/d1lc00266j
Akademický článek
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Autor:
Nikolaos Tairis, Mahdi Sarmady, Deborah Ferriola, Timothy Mosbruger, Monica D’Arcy, Chao Wu, Xiaowu Gai, Peter M Clark, Katarzyna Mackiewicz, Pushkala Jayaraman, Dimitrios Monos, Taishan Hu
Publikováno v:
Bioinformatics
SummaryA number of methods have been devised to address the need for targeted genomic resequencing. One of these methods, Region-specific extraction (RSE) of DNA is characterized by the capture of long DNA fragments (15-20 kb) by magnetic beads, afte
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::400371379dfafa3c7725406286232043
https://doi.org/10.1093/bioinformatics/btaa552
https://doi.org/10.1093/bioinformatics/btaa552
Publikováno v:
Biochimie. 168:259-267
Over the last two decades, isothermal amplification of nucleic acids has gained more attention due to a number of advantages over the widely used polymerase chain reaction. For isothermal amplification, DNA polymerases with strand-displacement activi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f45c2fd99f7ee0719663acef015366b3
https://doi.org/10.1016/j.biochi.2019.11.013
https://doi.org/10.1016/j.biochi.2019.11.013
Autor:
Ruliang Wang, Ya Su, Zhi Zheng, Rongxin Fu, Wenli Du, Guoliang Huang, Wenqi Lv, Xue Lin, Xiangyu Jin, Xiaohui Shan, Han Yang
Publikováno v:
ACS sensors. 6(11)
Nucleic acid detection plays a vital role in both biomedical research and clinical medicine. The temperature circulation changes of the widely used polymerase chain reaction technique are time-consuming and technically challenging for system developm
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::818e9a92c9e1d062ecad0e0bf2cafba6
https://pubmed.ncbi.nlm.nih.gov/34694791
https://pubmed.ncbi.nlm.nih.gov/34694791
Publikováno v:
Frontiers in Plant Science
Frontiers in Plant Science, Vol 12 (2021)
Frontiers in Plant Science, Vol 12 (2021)
Genotyping by sequencing (GBS) enables genotyping of multiple loci at low cost. However, the single nucleotide polymorphisms (SNPs) revealed by GBS tend to be randomly distributed between individuals, limiting their direct comparisons without applyin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6b7ff415cc0c9843974ab5ebef6f58a
Autor:
Daniel Hutter, Benjamin Overton, Chris McLendon, Jennifer T Le, Steven A. Benner, Kevin M. Bradley, Zunyi Yang
Publikováno v:
Biology Methods & Protocols
Despite its widespread value to molecular biology, the polymerase chain reaction (PCR) encounters modes that unproductively consume PCR resources and prevent clean signals, especially when high sensitivity, high SNP discrimination, and high multiplex
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::348ba0556999477fdec49821d2f9e4e6
http://europepmc.org/articles/PMC7200914
http://europepmc.org/articles/PMC7200914
Autor:
Wei Liang, Na Li, Fang Wang, Duobao Ge, Lei Wang, Siming Wang, Wenjun Zhu, Zhexiong Zhang, Huimin Chen
Publikováno v:
Analytical biochemistry. 633
Owing to modern lifestyles and increasing amounts of medical intervention, clinical infections caused by conditionally pathogenic fungi are becoming increasingly serious. Among these, Candida albicans is the most common. Therefore, the rapid and accu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56c1d5b94d9fabd4441e778092d8df5d
https://pubmed.ncbi.nlm.nih.gov/34678249
https://pubmed.ncbi.nlm.nih.gov/34678249