Zobrazeno 1 - 10
of 66
pro vyhledávání: '"Precise gene editing"'
Autor:
Di Li, Yaqi Cao, Long Xie, Chenfei He, Danrong Jiao, Mengxue Ma, Zhenrui Zuo, Erwei Zuo, Xiaogan Yang
Publikováno v:
Current Issues in Molecular Biology, Vol 46, Iss 5, Pp 4021-4034 (2024)
The demand for high-precision CRISPR/Cas9 systems in biomedicine is experiencing a notable upsurge. The editing system fdCas9 employs a dual-sgRNA strategy to enhance editing accuracy. However, the application of fdCas9 is constrained by the stringen
Externí odkaz:
https://doaj.org/article/21885f9c2de84230aa21ab8ff24f9c90
Autor:
Shenlin Hsiao, Shuanghong Chen, Yanhong Jiang, Qiudao Wang, Yang Yang, Yongrong Lai, Tao Zhong, Jiaoyang Liao, Yuxuan Wu
Publikováno v:
Advanced Science, Vol 11, Iss 30, Pp n/a-n/a (2024)
Abstract The current‐generation adenine base editor (ABE) ABE8e, which has evolved from the prokaryotic evolution system, exhibits high efficiency in mediating A‐to‐G conversion and is presumed to be promising for gene therapy. However, its muc
Externí odkaz:
https://doaj.org/article/cdc07ffdf3424fd789a9fc523774fbc8
Publikováno v:
BMC Biology, Vol 21, Iss 1, Pp 1-17 (2023)
Abstract Background Gene knockout and knock-in have been widely performed in large farm animals based on genome editing systems. However, many types of precise gene editing, including targeted deletion, gene tagging, and large gene fragment replaceme
Externí odkaz:
https://doaj.org/article/7a89b29a834a4dd6be08d91b8da714c5
Autor:
Yunjing Zhao, Xinyu Li, Chang Liu, Chaoqian Jiang, Xiaochen Guo, Qianqian Xu, Zhi Yin, Zhonghua Liu, Yanshuang Mu
Publikováno v:
Animals, Vol 14, Iss 5, p 719 (2024)
The aim of this study was to verify whether small molecules can improve the efficiency of precision gene editing using clustered regularly interspaced short palindromic repeats (CRISPR) ribonucleoprotein (RNP) in porcine cells. CRISPR associated 9 (C
Externí odkaz:
https://doaj.org/article/dbcea158bf6043cfa845b33030ea5789
Autor:
Sara Fañanas-Baquero, Matías Morín, Sergio Fernández, Isabel Ojeda-Perez, Mercedes Dessy-Rodriguez, Miruna Giurgiu, Juan A. Bueren, Miguel Angel Moreno-Pelayo, Jose Carlos Segovia, Oscar Quintana-Bustamante
Publikováno v:
Frontiers in Genome Editing, Vol 5 (2023)
Pyruvate kinase deficiency (PKD) is an autosomal recessive disorder caused by mutations in the PKLR gene. PKD-erythroid cells suffer from an energy imbalance caused by a reduction of erythroid pyruvate kinase (RPK) enzyme activity. PKD is associated
Externí odkaz:
https://doaj.org/article/9304ddc2d19f4b5a87e0230bce6ecc47
Autor:
Jialun Duan, Chunjie Bao, Ying Xie, Haitao Guo, Yixuan Liu, Jianwei Li, Rui Liu, Peishan Li, Jing Bai, Yan Yan, Limin Mu, Xueqi Li, Guiling Wang, Wanliang Lu
Publikováno v:
Bioactive Materials, Vol 11, Iss , Pp 1-14 (2022)
Clustered regularly interspaced short palindromic repeats (CRISPR) technology emerges a remarkable potential for cure of refractory cancer like metastatic breast cancer. However, how to efficiently deliver the CRISPR system with non-viral carrier rem
Externí odkaz:
https://doaj.org/article/2260eb1c78b24415972f8a711c26c132
Autor:
Wanqing Xu, Qingxia Zuo, Dongyan Feng, Changsheng He, Cailing Lin, Dongchao Huang, Yanbin Wan, Feng Chen, Guosheng Mo, Qi Sun, Hongli Du, Lizhen Huang
Publikováno v:
Current Issues in Molecular Biology, Vol 44, Iss 4, Pp 1688-1700 (2022)
An accurate visual reporter system to assess homology-directed repair (HDR) is a key prerequisite for evaluating the efficiency of Cas9-mediated precise gene editing. Herein, we tested the utility of the widespread promoterless EGFP reporter to asses
Externí odkaz:
https://doaj.org/article/4cee37ba11af425b8e62fcd55d6eb161
Akademický článek
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Publikováno v:
BMC Biotechnology, Vol 20, Iss 1, Pp 1-16 (2020)
Abstract Background Precise genetic modifications are preferred products of CRISPR-Cas9 mediated gene editing in mammalian cells but require the repair of induced double-strand breaks (DSB) through homology directed repair (HDR). Since HDR competes w
Externí odkaz:
https://doaj.org/article/494ce4496ccd4d98802430c0072600d6
Akademický článek
Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
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