Novel design for a microfluidic-based platform for yeast replicative lifespan (RLS) analysis

Autor: Georgia D. Kaprou, Abhay Andar, Pranjul Shah, Carole L. Linster, Nicole Paczia

Publikováno v: Micro and Nano Engineering, Vol 19, Iss , Pp 100199- (2023)

Microfluidic devices hold enormous potential for the development of cost-effective and faster alternatives to existing traditional methods across life science applications. Here we demonstrate the feasibility of fabricating a microfluidic device by m

Externí odkaz: https://doaj.org/article/b693211722844622935b085ff471d6ff

A microfluidics-based in vitro model of the gastrointestinal human–microbe interface

Autor: Pranjul Shah, Joëlle V. Fritz, Enrico Glaab, Mahesh S. Desai, Kacy Greenhalgh, Audrey Frachet, Magdalena Niegowska, Matthew Estes, Christian Jäger, Carole Seguin-Devaux, Frederic Zenhausern, Paul Wilmes

Publikováno v: Nature Communications, Vol 7, Iss 1, Pp 1-15 (2016)

Research on the interactions between the gut microbiota and human cells would greatly benefit from improved in vitro models. Here, Shah et al. present a modular microfluidics-based model that allows co-culture of human and microbial cells followed by

Externí odkaz: https://doaj.org/article/1c551f94bc624ad09b8ba6bc26235186

Metaphase FISH on a Chip: Miniaturized Microfluidic Device for Fluorescence in situ Hybridization

Autor: Niels Tommerup, Winnie E. Svendsen, Zeynep Tumer, Maria Dimaki, Pranjul Shah, Indumathi Vedarethinam

Publikováno v: Sensors, Vol 10, Iss 11, Pp 9831-9846 (2010)

Fluorescence in situ Hybridization (FISH) is a major cytogenetic technique for clinical genetic diagnosis of both inherited and acquired chromosomal abnormalities. Although FISH techniques have evolved and are often used together with other cytogenet

Externí odkaz: https://doaj.org/article/0786ef9cd2734dccb7e80e7c96ea27d7

A microfluidics-based in vitro model of the gastrointestinal human–microbe interface

Autor: Christian Jäger, Matthew Estes, Joëlle V. Fritz, Mahesh Desai, Frederic Zenhausern, Enrico Glaab, Carole Seguin-Devaux, Pranjul Shah, Audrey Frachet, Paul Wilmes, Magdalena Niegowska, Kacy Greenhalgh

Publikováno v: Nature Communications, Vol 7, Iss 1, Pp 1-15 (2016)
Nature Communications

Changes in the human gastrointestinal microbiome are associated with several diseases. To infer causality, experiments in representative models are essential, but widely used animal models exhibit limitations. Here we present a modular, microfluidics

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa1bb0c9a92eff175441af3c20b5176f
https://doaj.org/article/1c551f94bc624ad09b8ba6bc26235186

Sequential Isolation of DNA, RNA, Protein, and Metabolite Fractions from Murine Organs and Intestinal Contents for Integrated Omics of Host–Microbiota Interactions

Autor: Emilie E. L. Muller, Linda Wampach, Laura Lebrun, Paul Wilmes, Pranjul Shah

Publikováno v: Methods in Molecular Biology ISBN: 9781493986934
Microbial Proteomics, Methods and Protocols
Microbial Proteomics, Methods and Protocols, pp.279-291, 2018, ⟨10.1007/978-1-4939-8695-8_19⟩

The gastrointestinal microbiome plays a central role in health and disease. Imbalances in the microbiome, also referred to as dysbiosis, have recently been associated with a number of human idiopathic diseases ranging from metabolic to neurodegenerat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e49e9319eed58f99d6edc2400e8db14f
https://doi.org/10.1007/978-1-4939-8695-8_19