Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Por-Hsiung Lai"'
Publikováno v:
Biochemical Journal. 394:249-257
Human serum contains factors that promote oxidative folding of disulphide proteins. We demonstrate this here using hirudin as a model. Hirudin is a leech-derived thrombin-specific inhibitor containing 65 amino acids and three disulphide bonds. Oxidat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c1f988637f418d49640fe85a180388f
https://doi.org/10.1042/bj20051660
https://doi.org/10.1042/bj20051660
Autor:
Langley, Keith E.1, Por-Hsiung Lai1, Wypych, Jette1, Everett, Richard R.1, Berg, Thor F.1, Krabill, L. F.2, Davis, Janice M.1, Souza, Lawrence M.1
Publikováno v:
European Journal of Biochemistry. 3/2/87, Vol. 163 Issue 2, p323-330. 8p. 2 Diagrams, 2 Charts, 7 Graphs.
Publikováno v:
Journal of Biological Chemistry. 276:4845-4852
The folding pathway of human epidermal growth factor (EGF) has been characterized by structural and kinetic analysis of the acid-trapped folding intermediates. Oxidative folding of the fully reduced EGF proceeds through 1-disulfide intermediates and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb1ff9c6539cf06d1ee1e898797e898a
https://doi.org/10.1074/jbc.m005160200
https://doi.org/10.1074/jbc.m005160200
Publikováno v:
Protein Science. 8:1463-1468
Insulin-like growth factor (IGF-1) contains three disulfide bonds. In the presence of denaturant and thiol catalyst, IGF-1 shuffles its native disulfide bonds and denatures to form a mixture of scrambled isomers. The composition of scrambled IGF vari
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f971c2501d556f11d3c2c4ef0a27ccaf
https://doi.org/10.1110/ps.8.7.1463
https://doi.org/10.1110/ps.8.7.1463
Publikováno v:
The Journal of biological chemistry. 270(16)
Human epidermal growth factor (EGF) contains three disulfides and 53 amino acids. Reduced/denatured EGF refolds spontaneously in vitro to acquire its native structure. The mechanism of this folding process has been elucidated by structural analysis o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c9998f772c66f260184dbfbdfe8b3aea
https://pubmed.ncbi.nlm.nih.gov/7721838
https://pubmed.ncbi.nlm.nih.gov/7721838
Publikováno v:
Journal of Biological Chemistry. 261:3116-3121
Erythropoietin is the primary regulator of red blood cell formation in mammals. Because of its extreme scarcity, very little information is available regarding structural features of this important glycoprotein. We report here the primary structure o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bf6f025d848c4bb498161361fef9c458
https://doi.org/10.1016/s0021-9258(17)35756-3
https://doi.org/10.1016/s0021-9258(17)35756-3
Autor:
Michael Benjamin Mann, Dennis Fenton, Larry B. Tsai, Michael L. Klein, Craig Curless, Por-Hsiung Lai, Bruce W. Altrock, Hsieng Sen Lu, William C. Kenney
Publikováno v:
Biochemical and Biophysical Research Communications. 156:733-739
Interleukin-2 produced from a recombinant E. coli was found to contain as much as 19% norleucine in place of methionine in a minimal medium fermentation. Medium supplementation experiments and use of a leucine-requiring mutant host strain indicated t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a3417476f12446f9b0c40e589b4610c
https://doi.org/10.1016/s0006-291x(88)80904-5
https://doi.org/10.1016/s0006-291x(88)80904-5
Publikováno v:
Archives of Biochemistry and Biophysics. 268:81-92
Molecular characteristics and secondary structures of recombinant methionyl human granulocyte colony stimulating factor produced by genetically engineered Escherichia coli are described. Limited radiolabeling of the protein with tritiated iodoacetate
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae0cbbecdd7f4b800c1a23ff4686af5f
https://doi.org/10.1016/0003-9861(89)90567-5
https://doi.org/10.1016/0003-9861(89)90567-5
Autor:
Por-hsiung Lai
Publikováno v:
Analytica Chimica Acta. 163:243-248
Improved techniques for preparing samples and sequencing reagents have been developed to achieve highly sensitive protein sequencing. These improvements include a new sequencing buffer system which gives extremely low chromatographic background, a me
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::43a87312bf46e8a2795d3e0e6ec3d985
https://doi.org/10.1016/s0003-2670(00)81513-5
https://doi.org/10.1016/s0003-2670(00)81513-5
Publikováno v:
Biochemical and Biophysical Research Communications. 136:679-684
Recombinant human interferon-γ was phosphorylated with ATP and c-AMP-dependent protein kinase. After phosphorylation, interferon-γ was separated from the adenosine phosphates and the kinase and analyzed by SDS-PAGE, reverse phase HPLC, and HPLC pep
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0383c23e7c4e3d7a7c069a7a950b28f0
https://doi.org/10.1016/0006-291x(86)90494-8
https://doi.org/10.1016/0006-291x(86)90494-8