Zobrazeno 1 - 10
of 58
pro vyhledávání: '"Ponzy Lu"'
Publikováno v:
Molecular and Cellular Probes. 16:277-283
Current methods to detect and assay ribonuclease H (RNase H) activity are indirect and time-consuming. Here we introduce a direct and sensitive method, based on the fluorescence quenching mechanism of molecular beacons, to assay RNA cleavage in RNA:D
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b65a3ee31904ed4bc9d4f18ad50da1e
https://doi.org/10.1006/mcpr.2002.0423
https://doi.org/10.1006/mcpr.2002.0423
Publikováno v:
Nucleosides and Nucleotides. 16:1809-1820
A method to convert 5-FdU phosphoramidite to 4-triazol-5- FdU phosphoramidite was used to make deoxyoligonucleotides containing 5- FdC to follow a rare transition between a tandem hairpin and a single hairpin form, which results in moving a distant 5
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::96a2582d38f3e4e2324e91afe45a4cfd
https://doi.org/10.1080/07328319708002535
https://doi.org/10.1080/07328319708002535
Publikováno v:
Current Opinion in Structural Biology. 7:76-85
For many years the lac operon of Escherichia coli has been the paradigm for gene regulation. Recently, the structures of the lac repressor core bound to isopropyl-beta-D-1-thiogalactoside (IPTG), the intact apo lac repressor, the intact lac repressor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6156e01637e5443a881bb02c73c7987f
https://doi.org/10.1016/s0959-440x(97)80010-3
https://doi.org/10.1016/s0959-440x(97)80010-3
Publikováno v:
Journal of Molecular Biology. 265:1-7
The wild type E. coli lac operator is embedded in a 35 base-pair DNA sequence containing extensive 2-fold symmetry, suggesting a symmetric repressor operator complex. However, deviations from strict 2-fold symmetry occur at the central base-pair and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33f4284a950960891a1b9f5ca39eed8c
https://doi.org/10.1006/jmbi.1996.0706
https://doi.org/10.1006/jmbi.1996.0706
Publikováno v:
Nucleic Acids Research. 24:4015-4022
The oligonucleotide r(GGACUUCGGUCC) has been observed to adopt a hairpin conformation by solution NMR and a double helical conformation by X-ray diffraction. In order to understand this apparent conflict, we used time-resolved fluorescence depolariza
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33c872b0b69604ecd5e1504e3f9c1f4b
https://doi.org/10.1093/nar/24.20.4015
https://doi.org/10.1093/nar/24.20.4015
Publikováno v:
Nucleic Acids Research. 24:3911-3917
The Hepatitis Delta Virus (HDV) ribozyme self-cleaving activity in 20 M formamide solutions is unique. Does this catalytic activity result from the conservation of its tertiary structure in 20 M formamide? We followed the ribozyme structure in formam
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5e722ec4805946b2c538deebf4f8016
https://doi.org/10.1093/nar/24.20.3911
https://doi.org/10.1093/nar/24.20.3911
Publikováno v:
Journal of Molecular Recognition. 9:13-22
When the lac repressor tetramer is bound to its DNA operator, methylation protection shows the nearly symmetric operator half-sites are contracted asymmetrically. This asymmetric binding results from the DNA sequence/structure. The reported structure
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::595251fe79aa2b23b831141ec5e47df3
https://doi.org/10.1002/(sici)1099-1352(199601)9:1<13::aid-jmr234>3.0.co
https://doi.org/10.1002/(sici)1099-1352(199601)9:1<13::aid-jmr234>3.0.co
Publikováno v:
Separations Technology. 4:55-61
The objective of the present study was to examine the possibility of using a viscoelastic (free) solution to separate DNA molecules. The electrophoretic orientation of λ DNA in an uncross-linked polyacrylamide solution was measured by a fluorescence
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::90f6e77bdff4a1a39e40d1da2dd62cdc
https://doi.org/10.1016/0956-9618(94)80006-5
https://doi.org/10.1016/0956-9618(94)80006-5
Publikováno v:
Journal of Molecular Biology. 233:389-399
The Escherichia coli lac operator DNA contains two sequence repeats related by a pseudo-dyad axis. Deviations from symmetry, in the central 21 bp sequence, occur at two pairs of symmetrically related sites (+15/+7, +13/+9) and at the central base-pai
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c58bf6d1963ad06f015fc1ecf0e72be3
https://doi.org/10.1006/jmbi.1993.1519
https://doi.org/10.1006/jmbi.1993.1519
Autor:
Fraydoon Rastinejad, Ponzy Lu
Publikováno v:
Journal of Molecular Biology. 232:105-122
We have substituted 5-fluorodeoxyuridine (5-FdU) in place of thymidine in defined positions along synthetic bacteriophage T7 promoter DNA sequences. None of the fluoro-substitutions in the promoter DNA sequence reduced transcription yields with T7 RN
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::212a2e1d50a6e5ad860496cd88adef5a
https://doi.org/10.1006/jmbi.1993.1373
https://doi.org/10.1006/jmbi.1993.1373