Fixed-target serial femtosecond crystallography using in cellulo grown microcrystals

Autor: J. Mia Lahey-Rudolph, Robert Schönherr, Miriam Barthelmess, Pontus Fischer, Carolin Seuring, Armin Wagner, Alke Meents, Lars Redecke

Publikováno v: IUCrJ, Vol 8, Iss 4, Pp 665-677 (2021)

The crystallization of recombinant proteins in living cells is an exciting new approach in structural biology. Recent success has highlighted the need for fast and efficient diffraction data collection, optimally directly exposing intact crystal-cont

Externí odkaz: https://doaj.org/article/c5781ba8421e4c679d2e853c3604947f

Mix-and-diffuse serial synchrotron crystallography

Autor: Kenneth R. Beyerlein, Dennis Dierksmeyer, Valerio Mariani, Manuela Kuhn, Iosifina Sarrou, Angelica Ottaviano, Salah Awel, Juraj Knoska, Silje Fuglerud, Olof Jönsson, Stephan Stern, Max O. Wiedorn, Oleksandr Yefanov, Luigi Adriano, Richard Bean, Anja Burkhardt, Pontus Fischer, Michael Heymann, Daniel A. Horke, Katharina E. J. Jungnickel, Elena Kovaleva, Olga Lorbeer, Markus Metz, Jan Meyer, Andrew Morgan, Kanupriya Pande, Saravanan Panneerselvam, Carolin Seuring, Aleksandra Tolstikova, Julia Lieske, Steve Aplin, Manfred Roessle, Thomas A. White, Henry N. Chapman, Alke Meents, Dominik Oberthuer

Publikováno v: IUCrJ, Vol 4, Iss 6, Pp 769-777 (2017)

Unravelling the interaction of biological macromolecules with ligands and substrates at high spatial and temporal resolution remains a major challenge in structural biology. The development of serial crystallography methods at X-ray free-electron las

Externí odkaz: https://doaj.org/article/e490cd1b81b944869066336aa07f9314

Room-temperature macromolecular serial crystallography using synchrotron radiation

Autor: Francesco Stellato, Dominik Oberthür, Mengning Liang, Richard Bean, Cornelius Gati, Oleksandr Yefanov, Anton Barty, Anja Burkhardt, Pontus Fischer, Lorenzo Galli, Richard A. Kirian, Jan Meyer, Saravanan Panneerselvam, Chun Hong Yoon, Fedor Chervinskii, Emily Speller, Thomas A. White, Christian Betzel, Alke Meents, Henry N. Chapman

Publikováno v: IUCrJ, Vol 1, Iss 4, Pp 204-212 (2014)

A new approach for collecting data from many hundreds of thousands of microcrystals using X-ray pulses from a free-electron laser has recently been developed. Referred to as serial crystallography, diffraction patterns are recorded at a constant rate

Externí odkaz: https://doaj.org/article/10b13513eea84703be028145a9fb6894

X-ray screening identifies active site and allosteric inhibitors of SARS-CoV-2 main protease

Autor: M. Schwinzer, Faisal Hammad Mekky Koua, Ashwin Chari, J. Lieske, Maria Garcia-Alai, Gleb Bourenkov, Russell J. Cox, Salah Awel, W. Ewert, Dušan Turk, Luca Gelisio, N. Werner, Hévila Brognaro, J. Pletzer-Zelgert, Juraj Knoska, Arwen R. Pearson, D. Melo, Matthias Rarey, Ilona Dunkel, Boris Krichel, Y. Gevorkov, A. Tolstikova, David von Stetten, Eike C. Schulz, Andrea Zaliani, Winfried Hinrichs, F. Trost, Helen M. Ginn, Xinyuanyuan Sun, Stephan Niebling, Holger Fleckenstein, Aleksandra Usenik, J. Wollenhaupt, Robin Schubert, Stephan Günther, Kristina Lorenzen, J. Boger, P. Reinke, Diana C. F. Monteiro, Bruno Alves Franca, Isabel Bento, Manfred S. Weiss, Ariana Peck, Dominik Oberthuer, Pedram Mehrabi, Pontus Fischer, Sebastian Günther, Jure Loboda, P. Lourdu Xavier, C. Schmidt, Christian Betzel, Huijong Han, N. Ullah, Philip Gribbon, Aida Rahmani Mashhour, Charlotte Uetrecht, Guillaume Pompidor, Christiane Ehrt, Christian G. Feiler, Saravanan Panneerselvam, Bernhard Ellinger, Christian M. Günther, Thomas J. Lane, Linlin Zhang, S. Meier, Tobias Beck, Henry N. Chapman, M. Domaracky, Sven Falke, Katarina Karničar, Markus Wolf, Cromarte Rogers, S. Saouane, Ivars Karpics, Rolf Hilgenfeld, Gisel E. Peña-Murillo, Vasundara Srinivasan, Alke Meents, Miriam Barthelmess, M. Galchenkova, B. Seychell, Beatriz Escudero-Pérez, Thomas A. White, Janine-Denise Kopicki, Joanna J. Zaitseva-Doyle, W. Brehm, M. Groessler, Brenna Norton-Baker, Frank Schlünzen, Chufeng Li, Henning Tidow, Maria Kuzikov, Andrea R. Beccari, Johanna Hakanpää, Henry Gieseler, Yaiza Fernández-García, Oleksandr Yefanov, Thomas R. Schneider, Anna Hänle, Jan Meyer, H. Andaleeb, V. Hennicke

Publikováno v: Science (New York, N.y.)
Science 372 (6542): 642-646 (2021-05-07)
Science
Science / Science now 372(6542), 642-646 (2021). doi:10.1126/science.abf7945

A large-scale screen to target SARS-CoV-2 The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genome is initially expressed as two large polyproteins. Its main protease, Mpro, is essential to yield functional viral proteins, making it a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a7f9ac410aabd144f9e9014ed83aff5
https://resolver.caltech.edu/CaltechAUTHORS:20201113-100151878

Inhibition of SARS-CoV-2 main protease by allosteric drug-binding

Autor: Henry Gieseler, David von Stetten, A. Tolstikova, Luca Gelisio, Yaiza Fernández-García, Guillaume Pompidor, Saravanan Panneerselvam, Cromarte Rogers, M. Galchenkova, Juraj Knoska, B. Seychell, Gleb Bourenkov, Ariana Peck, Stephan Niebling, D. Melo, Hévila Brognaro, Russell J. Cox, Rolf Hilgenfeld, W. Brehm, J. Lieske, Matthias Rarey, Aleksandra Usenik, Tobias Beck, Oleksandr Yefanov, Henry N. Chapman, Isabel Bento, C. Schmidt, M. Groessler, Ashwin Chari, Sven Falke, Katarina Karničar, Thomas J. Lane, Beatriz Escudero-Pérez, J. Boger, Bernhard Ellinger, Christian M. Günther, Christiane Ehrt, Markus Wolf, Jure Loboda, Thomas R. Schneider, Andrea Zaliani, P. Lourdu Xavier, Eike C. Schulz, J. Wollenhaupt, Brenna Norton-Baker, Ivars Karpics, Salah Awel, Aida Rahmani Mashhour, Frank Schlünzen, Chufeng Li, Henning Tidow, Robin Schubert, Y. Gevorkov, H. Andaleeb, P. Reinke, Stephan Günther, Christian G. Feiler, Manfred S. Weiss, Jo J. Zaitsev-Doyle, N. Ullah, M. Domaracky, Pontus Fischer, Linlin Zhang, Maria Kuzikov, V. Hennicke, W. Ewert, Faisal Hammad Mekky Koua, P. Gribbon, Xinyuanyuan Sun, Dominik Oberthuer, S. Meier, J. Pletzer-Zelgert, Kristina Lorenzen, Christian Betzel, M. Schwinzer, Bruno Alves Franca, Charlotte Uetrecht, S. Saouane, Pedram Mehrabi, Helen M. Ginn, Andrea R. Beccari, Johanna Hakanpää, Diana C. F. Monteiro, Ilona Dunkel, Janine-Denise Kopicki, Anna Hänle, Miriam Barthelmess, Jan Meyer, Dušan Turk, N. Werner, Thomas A. White, Boris Krichel, Winfried Hinrichs, F. Trost, Sebastian Günther, Filip Guicking, Vasundara Srinivasan, Alke Meents, Gisel E. Peña Murillo, Maria Marta Garcia Alai, Huijong Han, Arwen R. Pearson, Holger Fleckenstein

The coronavirus disease (COVID-19) caused by SARS-CoV-2 is creating tremendous health problems and economical challenges for mankind. To date, no effective drug is available to directly treat the disease and prevent virus spreading. In a search for a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::78d640669dec95efad943e6c0a02e329
https://doi.org/10.1101/2020.11.12.378422