PAM-flexible genome editing with an engineered chimeric Cas9.

Autor: Zhao L; Department of Biomedical Engineering, Duke University, Durham, NC, USA., Koseki SRT; Department of Biomedical Engineering, Duke University, Durham, NC, USA., Silverstein RA; Center for Genomic Medicine, Massachusetts General Hospital, Boston, MA, USA.; Department of Pathology, Massachusetts General Hospital, Boston, MA, USA.; Biological and Biomedical Sciences Program, Harvard University, Boston, MA, USA., Amrani N; RNA Therapeutics Institute, University of Massachusetts Medical School, Cambridge, USA., Peng C; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Canada., Kramme C; Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA, USA., Savic N; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Canada., Pacesa M; Department of Biochemistry, University of Zurich, Zürich, Switzerland., Rodríguez TC; RNA Therapeutics Institute, University of Massachusetts Medical School, Cambridge, USA., Stan T; Department of Biomedical Engineering, Duke University, Durham, NC, USA., Tysinger E; Department of Biomedical Engineering, Duke University, Durham, NC, USA., Hong L; Department of Biomedical Engineering, Duke University, Durham, NC, USA., Yudistyra V; Department of Biomedical Engineering, Duke University, Durham, NC, USA., Ponnapati MR; Media Lab, Massachusetts Institute of Technology, Cambridge, MA, USA., Jacobson JM; Media Lab, Massachusetts Institute of Technology, Cambridge, MA, USA., Church GM; Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA, USA., Jakimo N; Media Lab, Massachusetts Institute of Technology, Cambridge, MA, USA., Truant R; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Canada., Jinek M; Department of Biochemistry, University of Zurich, Zürich, Switzerland., Kleinstiver BP; Center for Genomic Medicine, Massachusetts General Hospital, Boston, MA, USA.; Department of Pathology, Massachusetts General Hospital, Boston, MA, USA.; Department of Pathology, Harvard Medical School, Boston, MA, USA., Sontheimer EJ; RNA Therapeutics Institute, University of Massachusetts Medical School, Cambridge, USA., Chatterjee P; Department of Biomedical Engineering, Duke University, Durham, NC, USA. pranam.chatterjee@duke.edu.; Department of Computer Science, Duke University, Durham, NC, USA. pranam.chatterjee@duke.edu.

Publikováno v: Nature communications [Nat Commun] 2023 Oct 04; Vol. 14 (1), pp. 6175. Date of Electronic Publication: 2023 Oct 04.