Poly(ADP-Ribose) Glycohydrolase (PARG) vs. Poly(ADP-Ribose) Polymerase (PARP) – Function in Genome Maintenance and Relevance of Inhibitors for Anti-cancer Therapy

Autor: Daniel Harrision, Polly Gravells, Ruth Thompson, Helen E. Bryant

Publikováno v: Frontiers in Molecular Biosciences, Vol 7 (2020)

Poly(ADP-ribose) polymerases (PARPs) are a family of enzymes that catalyze the addition of poly(ADP-ribose) (PAR) subunits onto themselves and other acceptor proteins. PARPs are known to function in a large range of cellular processes including DNA r

Externí odkaz: https://doaj.org/article/04d95c5d2ace4596acecc99477fb1506

MYCN expression induces replication stress and sensitivity to PARP inhibition in neuroblastoma

Autor: Polly Gravells, Xiao Dun Li, Daniel Harrison, Yann Jamin, Evon Poon, Daniel Yeomanson, Saoirse Burke, David A. King, Simon P. Robinson, Sally L George, Colin Kwok, Christopher J. Lord, Albert Hallsworth, Helen E. Bryant, Louis Chesler, Gilberto S. Almeida

Publikováno v: Oncotarget

This study investigates the influence expression of the MYCN oncogene has on the DNA damage response, replication fork progression and sensitivity to PARP inhibition in neuroblastoma. In a panel of neuroblastoma cell lines, MYCN amplification or MYCN

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::578e0eb8f8c5e62f663a1650da37f58a
https://doi.org/10.18632/oncotarget.27329

DNA Fiber Assay for the Analysis of DNA Replication Progression in Human Pluripotent Stem Cells

Autor: Helen E. Bryant, Jason A. Halliwell, Polly Gravells

Publikováno v: Current Protocols in Stem Cell Biology. 54

Human pluripotent stem cells (PSC) acquire recurrent chromosomal instabilities during prolonged in vitro culture that threaten to preclude their use in cell-based regenerative medicine. The rapid proliferation of pluripotent cells leads to constituti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4cd2bba62e68c8f9bf1a751d2c9a528e
https://doi.org/10.1002/cpsc.115

Specific killing of DNA damage-response deficient cells with inhibitors of poly(ADP-ribose) glycohydrolase

Autor: Polly Gravells, Emma Grant, Helen E. Bryant, Dominic I. James, Kate M. Smith

Publikováno v: Gravells, P, Grant, E, Smith, K M, James, D I & Bryant, H E 2017, ' Specific killing of DNA damage-response deficient cells with inhibitors of poly(ADP-ribose) glycohydrolase ', DNA Repair, vol. 52, pp. 81-91 . https://doi.org/10.1016/j.dnarep.2017.02.010
DNA Repair

Highlights • A synthetic lethal screen for Poly(ADP-ribose)glycohydrolase (PARG) is presented. • SiRNA and the PARG inhibitors Gallotannin and PDD00017273 are used. • PARG is synthetically lethal with BRCA1, BRCA2, PALB2, FAM175A (ABRAXAS) and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2506779c80c57f6e374f1b389c67257c
http://www.scopus.com/inward/record.url?scp=85013774991&partnerID=8YFLogxK

Atypically low spontaneous sister chromatid exchange formation in uveal melanoma

Autor: Polly Gravells, David Canovas, Aliya Ul-Hassan, Karen Sisley, Helen E. Bryant, Ian G. Rennie, Leslie Hoh

Publikováno v: Genes, Chromosomes and Cancer. 50:34-42

Uveal melanoma (UM) is the most common primary intraocular cancer of adults and is characterized by several well-established chromosomal changes. More recently, a specific mutation of guanine nucleotide binding protein Gq alpha subunit (GNAQ) has als

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc634d847d98244301328bb54c339920
https://doi.org/10.1002/gcc.20829