Zobrazeno 1 - 10
of 50
pro vyhledávání: '"Polina V. Shcherbakova"'
Autor:
Daria Ostroverkhova, Kathrin Tyryshkin, Annette K. Beach, Elizabeth A. Moore, Yosef Masoudi-Sobhanzadeh, Stephanie R. Barbari, Igor B. Rogozin, Konstantin V. Shaitan, Anna R. Panchenko, Polina V. Shcherbakova
Publikováno v:
Cell Reports, Vol 43, Iss 1, Pp 113655- (2024)
Summary: Alterations in the exonuclease domain of DNA polymerase ε cause ultramutated cancers. These cancers accumulate AGA>ATA transversions; however, their genomic features beyond the trinucleotide motifs are obscure. We analyze the extended DNA c
Externí odkaz:
https://doaj.org/article/e3c6568d61ee42b79709d60182e96c2f
Autor:
Xuanxuan Xing, Daniel P. Kane, Chelsea R. Bulock, Elizabeth A. Moore, Sushma Sharma, Andrei Chabes, Polina V. Shcherbakova
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
Somatic alterations in the exonuclease domain of DNA polymerase ɛ have been linked to the development of highly mutated cancers. Here, the authors report that a major consequence of the most common cancer-associated Polɛ variant is a dramatically i
Externí odkaz:
https://doaj.org/article/35b8abf68c3c4cfebd40b90ae653eef7
Autor:
Vimal Parkash, Yashraj Kulkarni, Josy ter Beek, Polina V. Shcherbakova, Shina Caroline Lynn Kamerlin, Erik Johansson
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
Mutations in the human POLE gene are associated with tumours with high mutational loads. Here the authors provide a structural rationale for the mutagenic activity of the cancer-associated DNA polymerase ε P286R variant.
Externí odkaz:
https://doaj.org/article/347e31414f014ab19733e8e7a0a88110
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 8, Iss 3, Pp 1019-1029 (2018)
DNA replication fidelity relies on base selectivity of the replicative DNA polymerases, exonucleolytic proofreading, and postreplicative DNA mismatch repair (MMR). Ultramutated human cancers without MMR defects carry alterations in the exonuclease do
Externí odkaz:
https://doaj.org/article/3328b8b9ee294ca595041c948db117cd
Autor:
Kartik A. Shah, Alexander A. Shishkin, Irina Voineagu, Youri I. Pavlov, Polina V. Shcherbakova, Sergei M. Mirkin
Publikováno v:
Cell Reports, Vol 2, Iss 5, Pp 1088-1095 (2012)
Expansions of simple DNA repeats cause numerous hereditary diseases in humans. We analyzed the role of DNA polymerases in the instability of Friedreich’s ataxia (GAA)n repeats in a yeast experimental system. The elementary step of expansion corresp
Externí odkaz:
https://doaj.org/article/2f69603835824da29a6e489fc9c02e41
Autor:
Stephanie R Barbari, Annette K Beach, Joel G Markgren, Vimal Parkash, Elizabeth A Moore, Erik Johansson, Polina V Shcherbakova
Publikováno v:
Nucleic Acids Research. 50:8023-8040
Amino acid substitutions in the exonuclease domain of DNA polymerase ϵ (Polϵ) cause ultramutated tumors. Studies in model organisms suggested pathogenic mechanisms distinct from a simple loss of exonuclease. These mechanisms remain unclear for most
Autor:
Polina V. Shcherbakova, Daniel P. Kane
PDF file - 63KB, Supplementary Figure S1. Strategy for PCR amplification of the exonuclease domain region of POLE. Supplementary Table S1. Primers and reaction conditions used to amplify the entire coding sequence of POLE from tumor sample T-04-364 b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0fba99d6ac225689cbd74af748a9d686
https://doi.org/10.1158/0008-5472.22401273
https://doi.org/10.1158/0008-5472.22401273
Autor:
Polina V. Shcherbakova, Daniel P. Kane
Exonucleolytic proofreading and DNA mismatch repair (MMR) act in series to maintain high-fidelity DNA replication and to avoid mutagenesis. MMR defects elevate the overall mutation rate and are associated with increased cancer incidence. Hypermutable
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c5ec5f02a6740e42b3e7d5456a395bb7
https://doi.org/10.1158/0008-5472.c.6505803
https://doi.org/10.1158/0008-5472.c.6505803
Publikováno v:
Nucleic Acids Research
Substitutions in the exonuclease domain of DNA polymerase ϵ cause ultramutated human tumors. Yeast and mouse mimics of the most common variant, P286R, produce mutator effects far exceeding the effect of Polϵ exonuclease deficiency. Yeast Polϵ-P301
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America
Significance Polδ and Polε are the two major replicative polymerases in eukaryotes, but their precise roles at the replication fork remain a subject of debate. A bulk of data supports a model where Polε and Polδ synthesize leading and lagging DNA