Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Pohl Milon"'
Autor:
Thuy Duong Pham, Chiara Poletti, Therese Manuela Nloh Tientcheu, Massimiliano Cuccioloni, Roberto Spurio, Attilio Fabbretti, Pohl Milon, Anna Maria Giuliodori
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-16 (2024)
Abstract Cell-free protein synthesis (CFPS) systems offer a versatile platform for a wide range of applications. However, the traditional methods for detecting proteins synthesized in CFPS, such as radioactive labeling, fluorescent tagging, or electr
Externí odkaz:
https://doaj.org/article/7efb013c88e849d39b18fb47e9aa273f
Autor:
Ana Quiroz-Huanca, Ana Sanchez-Castro, Pablo Soriano-Castillo, Chiara Poletti, Therese Manuela Nloh Tientcheu, Attilio Fabbretti, Anna Maria Giuliodori, Pohl Milon
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103448- (2024)
Summary: Here, we present an in vitro protocol to assay mRNA translation inhibitors using the fluorescent assembly of split-GFP for translation test (FAST), based on the small fragment GFP11 binding to GFP1-10fast. We detail the expression and purifi
Externí odkaz:
https://doaj.org/article/377b6e1fe4954eb4a617591af978f478
Autor:
Patricia Julián, Pohl Milon, Xabier Agirrezabala, Gorka Lasso, David Gil, Marina V Rodnina, Mikel Valle
Publikováno v:
PLoS Biology, Vol 9, Iss 7, p e1001095 (2011)
Formation of the 30S initiation complex (30S IC) is an important checkpoint in regulation of gene expression. The selection of mRNA, correct start codon, and the initiator fMet-tRNA(fMet) requires the presence of three initiation factors (IF1, IF2, I
Externí odkaz:
https://doaj.org/article/ae451c619d1949adb886b4f57a66fb45
Autor:
Eva Dueñas, Jose A. Nakamoto, Luis Cabrera-Sosa, Percy Huaihua, María Cruz, Jorge Arévalo, Pohl Milón, Vanessa Adaui
Publikováno v:
Frontiers in Microbiology, Vol 13 (2022)
Tegumentary leishmaniasis, a disease caused by protozoan parasites of the genus Leishmania, is a major public health problem in many regions of Latin America. Its diagnosis is difficult given other conditions resembling leishmaniasis lesions and co-o
Externí odkaz:
https://doaj.org/article/7e004292ece04cd4b3603ac80ff569b9
Autor:
Letizia, Brandi, Attilio, Fabbretti, Pohl, Milon, Marcello, Carotti, Cynthia L, Pon, Claudio O, Gualerzi
Publikováno v:
Methods in enzymology. 431
This chapter presents methods and protocols suitable for the identification and characterization of inhibitors of the prokaryotic and/or eukaryotic translational apparatus as a whole or targeting specific, underexploited targets of the bacterial prot
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::25de4c9a17565004c20913dea8bc2db8
https://pubmed.ncbi.nlm.nih.gov/17923238
https://pubmed.ncbi.nlm.nih.gov/17923238
Autor:
Roberto Alcántara, Katherin Peñaranda, Gabriel Mendoza-Rojas, Jose A. Nakamoto, Eva Dueñas, Daniela Alvarez, Vanessa Adaui, Pohl Milón
Publikováno v:
STAR Protocols, Vol 2, Iss 4, Pp 100878- (2021)
Summary: Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers
Externí odkaz:
https://doaj.org/article/5f855e9ac88b4fc5bc14c0001c76f1d7
Autor:
Gabriel Mendoza-Rojas, Vanessa Sarabia-Vega, Ana Sanchez-Castro, Lesia Tello, Luis Cabrera-Sosa, Jose A. Nakamoto, Katherin Peñaranda, Vanessa Adaui, Roberto Alcántara, Pohl Milón
Publikováno v:
STAR Protocols, Vol 2, Iss 4, Pp 100899- (2021)
Summary: Here, we describe a detailed step-by-step protocol for the expression, purification, quantification, and activity determination of key enzymes for molecular detection of pathogens. Based on previous reports, we optimized the protocol for LbC
Externí odkaz:
https://doaj.org/article/ea9665530c934376806cf99d74794974
Autor:
Daniel J. Bennison, Jose A. Nakamoto, Timothy D. Craggs, Pohl Milón, John B. Rafferty, Rebecca M. Corrigan
Publikováno v:
mBio, Vol 12, Iss 6 (2021)
ABSTRACT During nutrient limitation, bacteria produce the alarmones (p)ppGpp as effectors of a stress signaling network termed the stringent response. RsgA, RbgA, Era, and HflX are four ribosome-associated GTPases (RA-GTPases) that bind to (p)ppGpp i
Externí odkaz:
https://doaj.org/article/30d2fbf8331d4dca84c56f46e5ca1dd8
Publikováno v:
Frontiers in Microbiology, Vol 12 (2021)
Tetracycline has positively impacted human health as well as the farming and animal industries. Its extensive usage and versatility led to the spread of resistance mechanisms followed by the development of new variants of the antibiotic. Tetracycline
Externí odkaz:
https://doaj.org/article/1e3d5903ecd04014af2da3866347bbc8
Autor:
Elena M. Maksimova, Daria S. Vinogradova, Ilya A. Osterman, Pavel S. Kasatsky, Oleg S. Nikonov, Pohl Milón, Olga A. Dontsova, Petr V. Sergiev, Alena Paleskava, Andrey L. Konevega
Publikováno v:
Frontiers in Microbiology, Vol 12 (2021)
Amicoumacin A (Ami) halts bacterial growth by inhibiting the ribosome during translation. The Ami binding site locates in the vicinity of the E-site codon of mRNA. However, Ami does not clash with mRNA, rather stabilizes it, which is relatively unusu
Externí odkaz:
https://doaj.org/article/aa4de2bb342943d5946698d32f450c37