Automated High-Throughput Matrix Assisted Laser Desorption Ionization Mass Spectrometry Methodology for Formulation Assessment of Polyethylene-Glycol-Conjugated Cytokine Proteins.

Autor: Pirrone GF; Merck & Co., Inc., Analytical Research & Development, 33 Avenue Louis Pasteur, Boston, MA, USA. Electronic address: gregory.pirrone@merck.com., Munsell EV; Merck & Co., Inc., Discovery Pharmaceutical Sciences, 33 Avenue Louis Pasteur, Boston, MA, USA., Ferguson HM; Merck & Co., Inc., Discovery Pharmaceutical Sciences, 33 Avenue Louis Pasteur, Boston, MA, USA., Al-Sayah MA; Merck & Co., Inc., Analytical Research & Development, 33 Avenue Louis Pasteur, Boston, MA, USA., Luthra SA; Merck & Co., Inc., Discovery Pharmaceutical Sciences, 33 Avenue Louis Pasteur, Boston, MA, USA., Makarov AA; Merck & Co., Inc., Analytical Research & Development, 33 Avenue Louis Pasteur, Boston, MA, USA. Electronic address: alexey.makarov@merck.com.

Publikováno v: Journal of pharmaceutical sciences [J Pharm Sci] 2023 Nov; Vol. 112 (11), pp. 2778-2782. Date of Electronic Publication: 2023 Jul 20.

Rapid antibody conformational screening by matrix-assisted laser desorption/ionization hydrogen-deuterium exchange mass spectrometry.

Autor: Mukherjee D; Merck & Co. Inc., Merck Research Laboratories, Analytical Research & Development, Boston, Massachusetts, USA., Trigo-Mouriño P; Merck & Co. Inc., Merck Research Laboratories, Analytical Research & Development, Boston, Massachusetts, USA., Jiang Y; Merck & Co. Inc., Merck Research Laboratories, Analytical Research & Development, Boston, Massachusetts, USA., Nowak T; Merck & Co. Inc., Merck Research Laboratories, Analytical Research & Development, Boston, Massachusetts, USA., Shchurik V; Merck & Co. Inc., Merck Research Laboratories, Analytical Research & Development, Boston, Massachusetts, USA., Adpressa DA; Merck & Co. Inc., Merck Research Laboratories, Analytical Research & Development, Boston, Massachusetts, USA., Louie MT; Absci Corp, Vancouver, Washington, USA., Reynolds SR; Absci Corp, Vancouver, Washington, USA., Hohn MJ; Merck & Co. Inc., Merck Research Laboratories, Bioprocess Research & Development, Boston, Massachusetts, USA., Al-Sayah MA; Merck & Co. Inc., Merck Research Laboratories, Analytical Research & Development, Boston, Massachusetts, USA., Pirrone GF; Merck & Co. Inc., Merck Research Laboratories, Analytical Research & Development, Boston, Massachusetts, USA., Makarov AA; Merck & Co. Inc., Merck Research Laboratories, Analytical Research & Development, Boston, Massachusetts, USA.

Publikováno v: Journal of separation science [J Sep Sci] 2022 Jun; Vol. 45 (12), pp. 2055-2063. Date of Electronic Publication: 2022 Feb 16.

Combination of HDX-MS and in silico modeling to study enzymatic reactivity and stereo-selectivity at different solvent conditions.

Autor: Makarov AA; Merck & Co., Inc., Process Research & Development, Rahway, NJ 07065, USA. Electronic address: alexey.makarov@merck.com., Iacob RE; Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA., Pirrone GF; Merck & Co., Inc., Process Research & Development, Rahway, NJ 07065, USA., Rodriguez-Granillo A; Merck & Co., Inc., Computational and Structural Chemistry, Rahway, NJ 07065, USA., Joyce L; Merck & Co., Inc., Process Research & Development, Rahway, NJ 07065, USA., Mangion I; Merck & Co., Inc., Process Research & Development, Rahway, NJ 07065, USA., Moore JC; Merck & Co., Inc., Process Research & Development, Rahway, NJ 07065, USA., Sherer EC; Merck & Co., Inc., Computational and Structural Chemistry, Rahway, NJ 07065, USA., Engen JR; Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA.

Publikováno v: Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2020 Apr 15; Vol. 182, pp. 113141. Date of Electronic Publication: 2020 Feb 01.

Use of MALDI-MS with solid-state hydrogen deuterium exchange for semi-automated assessment of peptide and protein physical stability in lyophilized solids.

Autor: Kabaria SR; Merck & Co., Inc., Merck Research Laboratories, Process Research & Development, Rahway, NJ, 07065, USA; Robert F. Smith School of Chemical and Biomolecular Engineering, Cornell University, 120 Olin Hall, Ithaca, NY, 14853, USA., Mangion I; Merck & Co., Inc., Merck Research Laboratories, Process Research & Development, Rahway, NJ, 07065, USA., Makarov AA; Merck & Co., Inc., Merck Research Laboratories, Process Research & Development, Rahway, NJ, 07065, USA. Electronic address: alexey.makarov@merck.com., Pirrone GF; Merck & Co., Inc., Merck Research Laboratories, Process Research & Development, Rahway, NJ, 07065, USA. Electronic address: gregory.pirrone@merck.com.

Publikováno v: Analytica chimica acta [Anal Chim Acta] 2019 Apr 25; Vol. 1054, pp. 114-121. Date of Electronic Publication: 2019 Jan 09.

Evaluation of global conformational changes in peptides and proteins following purification by supercritical fluid chromatography.

Autor: Schiavone NM; Merck & Co., Inc., MRL, Analytical Research & Development, Rahway, NJ 07065, USA., Bennett R; Merck & Co., Inc., MRL, Analytical Research & Development, Rahway, NJ 07065, USA., Hicks MB; Merck & Co., Inc., MRL, Analytical Research & Development, Rahway, NJ 07065, USA., Pirrone GF; Merck & Co., Inc., MRL, Analytical Research & Development, Rahway, NJ 07065, USA., Regalado EL; Merck & Co., Inc., MRL, Analytical Research & Development, Rahway, NJ 07065, USA., Mangion I; Merck & Co., Inc., MRL, Analytical Research & Development, Rahway, NJ 07065, USA., Makarov AA; Merck & Co., Inc., MRL, Analytical Research & Development, Rahway, NJ 07065, USA. Electronic address: alexey.makarov@merck.com.

Publikováno v: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2019 Mar 15; Vol. 1110-1111, pp. 94-100. Date of Electronic Publication: 2019 Feb 13.