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Selection of reference genes for normalization of RT-qPCR data in gene expression studies in Anthonomus eugenii Cano (Coleoptera: Curculionidae).

Autor: Pinheiro, Daniele H.^1,2, Siegfried, Blair D.¹ bsiegfried1@ufl.edu

Publikováno v: Scientific Reports. 3/19/2020, Vol. 10 Issue 1, p1-12. 12p.

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An ex vitro hairy root system from petioles of detached soybean leaves for in planta screening of target genes and CRISPR strategies associated with nematode bioassays.

Autor: Freitas-Alves NS; Bioprocess Engineering and Biotechnology Graduate Program, Federal University of Paraná-UFPR, Curitiba, PR, Brazil.; Embrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-917, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., Moreira-Pinto CE; Embrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-917, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., Arraes FBM; Embrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-917, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., Costa LSL; Embrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-917, Brazil.; Molecular Biology Graduate Program, University of Brasília-UNB, Brasília, DF, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., de Abreu RA; Embrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-917, Brazil., Moreira VJV; Embrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-917, Brazil.; Molecular Biology Graduate Program, University of Brasília-UNB, Brasília, DF, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., Lourenço-Tessutti IT; Embrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-917, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., Pinheiro DH; Embrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-917, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., Lisei-de-Sa ME; Embrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-917, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., Paes-de-Melo B; Embrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-917, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., Pereira BM; Embrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-917, Brazil., Guimaraes PM; Embrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-917, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., Brasileiro ACM; Embrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-917, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., de Almeida-Engler J; INRAE, Université Côte d'Azur, CNRS, 06903, Sophia Antipolis, ISA, France.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., Soccol CR; Bioprocess Engineering and Biotechnology Graduate Program, Federal University of Paraná-UFPR, Curitiba, PR, Brazil., Morgante CV; Embrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-917, Brazil.; Embrapa Semiarid, Petrolina, PE, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., Basso MF; Embrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-917, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., Grossi-de-Sa MF; Bioprocess Engineering and Biotechnology Graduate Program, Federal University of Paraná-UFPR, Curitiba, PR, Brazil. fatima.grossi@embrapa.br.; Embrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-917, Brazil. fatima.grossi@embrapa.br.; Molecular Biology Graduate Program, University of Brasília-UNB, Brasília, DF, Brazil. fatima.grossi@embrapa.br.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil. fatima.grossi@embrapa.br.; Catholic University of Brasília, Brasília, DF, Brazil. fatima.grossi@embrapa.br.

Publikováno v: Planta [Planta] 2023 Dec 18; Vol. 259 (1), pp. 23. Date of Electronic Publication: 2023 Dec 18.