In vitro neutralisation of rotavirus infection by two broadly specific recombinant monovalent llama-derived antibody fragments.

Autor: Farah Aladin, Alexandra W C Einerhand, Janneke Bouma, Sandra Bezemer, Pim Hermans, Danielle Wolvers, Kate Bellamy, Leon G J Frenken, Jim Gray, Miren Iturriza-Gómara

Publikováno v: PLoS ONE, Vol 7, Iss 3, p e32949 (2012)

Rotavirus is the main cause of viral gastroenteritis in young children. Therefore, the development of inexpensive antiviral products for the prevention and/or treatment of rotavirus disease remains a priority. Previously we have shown that a recombin

Externí odkaz: https://doaj.org/article/cfee33b3b4b74efe892dd4a8a635883e

Leveraging single-domain antibodies and magnetic bead technology to improve manufacturing of T-cells for cell therapies

Autor: T. Stav-Noraas, T. Holt Herreng, S. Kjær, H. Almåsbak, K.E. Bernstroem, K. Alfsnes, E. Klijs, H. Adams, C. Wielders, Grethe Økern, D. Gjolberg, O. Amellem, Pim Hermans, J. de Rooij, L. Sierkstra

Publikováno v: Cytotherapy. 23:S95

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1714de385569a9b5994797c38be4692a
https://doi.org/10.1016/s1465324921004175

Structural Basis of Enhanced Crystallizability Induced by a Molecular Chaperone for Antibody Antigen-Binding Fragments

Autor: June Ereño-Orbea, Taylor Sicard, Hong Cui, Jacob Carson, Pim Hermans, Jean-Philippe Julien

Publikováno v: Journal of molecular biology. 430(3)

Monoclonal antibodies constitute one of the largest groups of drugs to treat cancers and immune disorders, and are guiding the design of vaccines against infectious diseases. Fragments antigen-binding (Fabs) have been preferred over monoclonal antibo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa35ea8fa95ece5f7f90499c41a698d2
https://pubmed.ncbi.nlm.nih.gov/29277294

Accelerating the clinical development of protein-based vaccines for malaria by efficient purification using a four amino acid C-terminal 'C-tag'

Autor: Jing, Jin, Kathryn A, Hjerrild, Sarah E, Silk, Rebecca E, Brown, Geneviève M, Labbé, Jennifer M, Marshall, Katherine E, Wright, Sandra, Bezemer, Stine B, Clemmensen, Sumi, Biswas, Yuanyuan, Li, Aadil, El-Turabi, Alexander D, Douglas, Pim, Hermans, Frank J, Detmers, Willem A, de Jongh, Matthew K, Higgins, Rebecca, Ashfield, Simon J, Draper

Publikováno v: International Journal for Parasitology

Graphical abstract
Highlights • Fusion of a four amino acid ‘C-tag’ allows purification of a PfRH5 malaria vaccine. • Overall process yield of 40–45% and very high product purity (>99%) was achieved. • His6-tagged and C-tagged PfRH5

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::41e4e74667fa11c3a7aa48c62d68b1c5
https://pubmed.ncbi.nlm.nih.gov/28153778

Muscle-specific kinase myasthenia gravis IgG4 autoantibodies cause severe neuromuscular junction dysfunction in mice

Autor: Kevin Sleijpen, Marc H. De Baets, Maartje G. Huijbers, Rinse Klooster, Kirsten R. Straasheijm, Silvère M. van der Maarel, Jaap J. Plomp, Jan J.G.M. Verschuuren, Frank Detmers, Aad Verrips, Erik H. Niks, Pilar Martinez-Martinez, Mario Losen, Pim Hermans

Publikováno v: Brain, 135, 1081-101
Brain, 135, Pt 4, pp. 1081-101
Brain, 135, 1081-1101. Oxford University Press
BRAIN, 135, 1081-1101
Brain, 135, 1081-1101

Item does not contain fulltext Myasthenia gravis is a paralytic disorder with autoantibodies against acetylcholine receptors at the neuromuscular junction. A proportion of patients instead has antibodies against muscle-specific kinase, a protein esse

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84f589248cc18ae63f88ae8a6975fd21
https://doi.org/10.1093/brain/aws025