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of 49
pro vyhledávání: '"Pieter L. deHaseth"'
Autor:
Mary E. Karpen, Pieter L. deHaseth
Publikováno v:
Biomolecules, Vol 5, Iss 2, Pp 668-678 (2015)
In the process of transcription initiation, the bacterial RNA polymerase binds double-stranded (ds) promoter DNA and subsequently effects strand separation of 12 to 14 base pairs (bp), including the start site of transcription, to form the so-called
Externí odkaz:
https://doaj.org/article/1fc58b5f686d4ef1b9ba21c47740d0b3
Autor:
Pieter L. deHaseth, Jonatha M. Gott
Publikováno v:
Molecular Microbiology. 75:543-546
In promoter DNA, the preferred distance of the -10 and -35 elements for interacting with RNA polymerase-bound sigma(70) is 17 bp. However, the Devi et al. paper in this issue of Molecular Microbiology demonstrates that when the C-terminal domain of s
Publikováno v:
Genes & Development. 23:2426-2436
In bacteria, multiple σs direct RNA polymerase to distinct sets of promoters. Housekeeping σs direct transcription from thousands of promoters, whereas most alternative σs are more selective, recognizing more highly conserved promoter motifs. For
Autor:
Pieter L. deHaseth, Olga V. Kourennaia
Publikováno v:
Journal of Bacteriology. 189:8430-8436
The heat shock sigma factor (σ 32 in Escherichia coli ) directs the bacterial RNA polymerase to promoters of a specific sequence to form a stable complex, competent to initiate transcription of genes whose products mitigate the effects of exposure o
Autor:
Pieter L. deHaseth, Victoria M. Cook
Publikováno v:
Journal of Biological Chemistry. 282:21319-21326
Formation of the strand-separated, open complex between RNA polymerase and a promoter involves several intermediates, the first being the closed complex in which the DNA is fully base-paired. This normally short lived complex has been difficult to st
Publikováno v:
Nucleic Acids Research
Formation of the stable, strand separated, 'open' complex between RNA polymerase and a promoter involves DNA melting of approximately 14 base pairs. The likely nucleation site is the highly conserved -11A base in the non-template strand of the -10 pr
Publikováno v:
Journal of Bacteriology. 187:6762-6769
Upon the exposure of Escherichia coli to high temperature (heat shock), cellular levels of the transcription factor σ 32 rise greatly, resulting in the increased formation of the σ 32 holoenzyme, which is capable of transcription initiation at heat
Autor:
Yang Wang, Pieter L. deHaseth
Publikováno v:
Journal of Bacteriology. 185:5800-5806
The Escherichia coli transcription factor sigma 32 binds to core RNA polymerase to form the holoenzyme responsible for transcription initiation at heat shock promoters, utilized upon exposure of the cell to higher temperatures. We have developed two
Publikováno v:
Proceedings of the National Academy of Sciences. 99:3493-3498
RNA polymerase forms competitor-resistant complexes with “forked DNA” templates that are double-stranded from the −35 promoter region through the first base pair of the −10 region, with an additional unpaired A at the 3′ end of the nontempl
The family of agn alleles in Escherichia coli pathovars encodes autotransporters that have been implicated in biofilm formation, autoaggregation, and attachment to cells. The alleles all have long leader RNAs preceding the Ag43 translation initiation
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d36606283bb2e304194df0a4a083970c
https://europepmc.org/articles/PMC4135670/
https://europepmc.org/articles/PMC4135670/