Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Pierre-François Tosi"'
Autor:
Johann Odot, Pierre-François Tosi, Grégory Gatouillat, Claudie Madoulet, Emilie Balasse, María Pilar López-Deber, David T. Hickman, Claude Nicolau
Publikováno v:
Cancer Letters. 257:165-171
The clinical use of chemotherapy in cancer treatment is limited by the occurrence of multidrug resistance (MDR) associated with the overexpression of membrane transporters, one of the best known is P-glycoprotein (Pgp), that actively expels drugs out
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6054adbcffbe754d2903236183f0eff
https://doi.org/10.1016/j.canlet.2007.04.001
https://doi.org/10.1016/j.canlet.2007.04.001
Autor:
Pierre-François Tosi, Laura Perrin, Johann Odot, Grégory Gatouillat, Claudie Madoulet, Emilie Balasse, Claude Nicolau
Publikováno v:
Biochemical and Biophysical Research Communications. 358:325-330
Overexpression of the 170 kDa plasma membrane P-glycoprotein (P-gp) represents the most common MDR mechanism in chemotherapy. In this work, specific autoantibodies to fragments from extracellular loops 1, 2, and 4 of the murine MDR1 P-gp were elicite
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d14a339e1e966a29c4de737b5332c590
https://doi.org/10.1016/j.bbrc.2007.04.117
https://doi.org/10.1016/j.bbrc.2007.04.117
Publikováno v:
Journal of Fluorescence. 1:141-146
A specific, rapid, and sensitive method for the detection of CD4 in solution was developed using pairs of fluorescently stained monoclonal antibodies which do not cross-compete. The assay is quantitated by flow cytometry using Simply Cellular microbe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::71dbd8008bd4e1bdff31a7e7a608854c
https://doi.org/10.1007/bf00865210
https://doi.org/10.1007/bf00865210
Autor:
Youssef Mouneimne, Pierre-François Tosi, Claude Nicolau, Loyd Sneed, David J. Volsky, Michael Zeira, Jaime E. Lazarte
Publikováno v:
Proceedings of the National Academy of Sciences. 88:4409-4413
Recombinant full-length CD4 expressed in Spodoptera frugiperda 9 cells with the baculovirus system was electroinserted in erythrocyte (RBC) membranes. Of the inserted CD4, 70% was "correctly" oriented as shown by fluorescence quenching experiments wi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2151b8506b98667db72b71b56fe28646
https://doi.org/10.1073/pnas.88.10.4409
https://doi.org/10.1073/pnas.88.10.4409
Publikováno v:
FEBS letters. 359(1)
Concentration-dependent electroinsertion of recombinant human membrane CD4 in human erythrocytes shows a saturation at an average of about 3,500 inserted CD4 epitopes per cell, detectable by flow cytometry. The erythrocyte recovery drops to 10% at th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d2026bc439dd6e9480646dd0225209d
https://pubmed.ncbi.nlm.nih.gov/7531654
https://pubmed.ncbi.nlm.nih.gov/7531654
Publikováno v:
Biotechnology and bioengineering. 40(2)
The baculovirus-insect cell system is reliable in expressing a variety of recombinant proteins. A recombinant baculovirus encoding the full length human CD4 has been used to infect Spodoptera frugiperda 9 cells in 6-L-airlift fermentors. The procedur
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::363217af53dfbe2b7c05581c12d04e4a
https://pubmed.ncbi.nlm.nih.gov/18601106
https://pubmed.ncbi.nlm.nih.gov/18601106
Autor:
David J. Volsky, Pierre-François Tosi, Youssef Mouneimne, Claude Nicolau, Loyd Sneed, Jaime E. Lazarte, Michael Zeira
Publikováno v:
Advances in Molecular Biology and Targeted Treatment for AIDS ISBN: 9781468459302
Infection by human immunodeficiency virus (HIV) 1–3 is mediated by binding to CD4, a glycoprotein expressed on the surface of HIV-susceptible cells.4 The interaction between CD4 and the HIV envelope glycoprotein, gp120, was demonstrated by McDougal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c78d08c46e3c0df5bdfdeab2cd53420d
https://doi.org/10.1007/978-1-4684-5928-9_26
https://doi.org/10.1007/978-1-4684-5928-9_26
Publikováno v:
Biochimica et biophysica acta. 1027(1)
Electroinsertion is a novel technique of protein implantation in cell membranes using electrical pulses, of field strength between 1.3 kV/cm and 2.1 kV/cm and up to 1 ms duration. The full length recombinant CD4 receptor could thus be inserted in hum
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4596e316fdd5b1e1d6d827df66c27cd
https://pubmed.ncbi.nlm.nih.gov/2204427
https://pubmed.ncbi.nlm.nih.gov/2204427
Autor:
F. B. Hollinger, Pierre-François Tosi, C. Lahart, Youssef Mouneimne, D. Dimitrov, Claude Nicolau
Publikováno v:
Journal of Acquired Immune Deficiency Syndromes & Human Retrovirology. 9:126
Membrane recombinant CD4 was electroinserted into the plasma membrane of red blood cells (RBCs) from four HIV patients. CD4 had been labeled with 125I before electroinsertion. The RBCs-CD4-125I were labeled with 51Cr and autotransfused to the donor p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a8e08e52edaec76dbe1ef991441ec296
https://doi.org/10.1097/00042560-199506000-00004
https://doi.org/10.1097/00042560-199506000-00004