Zobrazeno 1 - 10
of 25
pro vyhledávání: '"Pierre Prentki"'
Publikováno v:
Gene, Vol. 142, No 1 (1994) pp. 97-102
The DNA sequence of the entire apxI operon from Actinobacillus pleur opneumoniae serotype 1 reference strain 4074 has been determined. This 8292-bp fragment of the chromosomal DNA contains four open reading frames (ORFs) of the strongly hemolytic Apx
Autor:
Pierre Prentki, Frédéric Boccard
Publikováno v:
EMBO Journal, Vol. 12, No 13 (1993) pp. 5019-5027
Europe PubMed Central
Europe PubMed Central
The prokaryotic REP (repetitive extragenic palindromes) or PU (palindromic units) sequences are often associated with other repetitive elements, forming arrangements which have been called 'BIMEs' (bacterial interspersed mosaic elements). It is estim
Autor:
Pierre Prentki, Andrea Boffini
Publikováno v:
Electrophoresis, Vol. 14 (1993) pp. 259-265
We have developed a gel electrophoretic approach for visualizing and cloning protein binding sites from complete genomes. This system consists of a simple two-dimensional band shift, in which protein-DNA complexes are retarded in the first dimension,
Autor:
Nelly Ekaterinaki, Pierre Prentki
Publikováno v:
Research in Microbiology. 142:381-387
Escherichia coli cells are unable to co-maintain a λdv and a minimal pSC101 replicon. Selection for both plasmids results in a much reduced growth rate of the cells, whereas selection for one plasmid results in a rapid loss of the other. A chromosom
Publikováno v:
Molecular Microbiology, Vol. 4, No 9 (1990) pp. 1477-1486
The IS 1-encoded protein InsA binds specifically to both ends of IS 1, and acts as a repressor of IS1 gene expression and may be a direct inhibitor of the transposition process. We show here, using DNasel 'foot-printing' and gel retardation, that the
Publikováno v:
Nucleic Acids Research, Vol. 23, No 15 (1995) pp. 2959-2965
The histone-like protein integration host factor (IHF) of Escherichia coli binds to specific binding sites on the chromosome or on mobile genetic elements, and is involved in many cellular processes. We have analyzed the interaction of IHF with five
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac7a30ea0949a1d1e6598e08e948a1fa
https://archive-ouverte.unige.ch/unige:130899
https://archive-ouverte.unige.ch/unige:130899
Publikováno v:
Gene, Vol. 103, No 1 (1991) pp. 17-23
We have constructed two plasmid vectors which allow selection for in vivo deletions within cloned DNA fragments. The plasmids are derivatives of pBR322 which carry the Escherichia coli rpsL (strA) gene, known to confer a dominant streptomycin (Sm)-se
Autor:
Andrea Boffini, Pierre Prentki
Publikováno v:
Nucleic Acids Research, Vol. 19, No 7 (1991) pp. 1369-1374
We describe a simple two-dimensional electrophoresis procedure to identify the recognition sites of DNA-binding proteins within large DNA molecules. Using this approach, we have mapped E. coli IHF (Integration Host Factor) binding sites within phage
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::614abf83e624970b458a02446f55850b
https://europepmc.org/articles/PMC333888/
https://europepmc.org/articles/PMC333888/
Autor:
Pierre Prentki
Publikováno v:
Gene, Vol. 122, No 1 (1992) pp. 231-232
Sequencing shows that the ΔM15 mutation of the lacZ gene of Escherichia coli is an in-phase deletion which fuses codon 11 to 42.
Publikováno v:
Gene. 61:91-101
We have constructed artificial IS 1 -based transposons by attaching synthetic oligodeoxynucleotides, corresponding to the sequence of the ends of IS 1 , to a selectable DNA segment [‘Ω’ fragment; Prentki and Krisch, Gene 29 (1984) 303–313]. Th