Zobrazeno 1 - 10
of 124
pro vyhledávání: '"Pierre Jost"'
Publikováno v:
BioTechniques, Vol 27, Iss 4, Pp 744-752 (1999)
The study of changes in genome-wide levels of DNA methylation has become a key focus for understanding the epigenetic regulation of gene expression. Many procedures exist to study DNA methylation, falling into two categories: gene-specific and genome
Externí odkaz:
https://doaj.org/article/a98f55ce3fa34fbea87c2d48782bc8bd
Publikováno v:
BioTechniques, Vol 24, Iss 6, Pp 981-990 (1998)
A procedure for the efficient transfer of cell monolayers, cultured on glass coverslips, to microscopy slides has been developed. This technique involves the coating of the upper surface of an ethanol-fixed cultured cell layer with a film of collodio
Externí odkaz:
https://doaj.org/article/e06be91cb7544a82b6d5d3a6ff099346
Autor:
Frances V. Fuller-Pace, Stéphane Thiry, Michel Siegmann, Herbert Angliker, Hans Stahl, Jean Pierre Jost, Steffen Schwarz, Daniel Hess
We have shown previously that DNA demethylation by chick embryo 5-methylcytosine (5-MeC)-DNA glycosylase needs both protein and RNA. Amino acid sequences of nine peptides derived from a highly purified 5-MeC-DNA glycosylase complex were identified by
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::403d4c357fd8e6868fba670f0611ced2
http://doc.rero.ch/record/291554/files/27-16-3245.pdf
http://doc.rero.ch/record/291554/files/27-16-3245.pdf
We have previously shown that DNA demethylation by chick embryo 5-methylcytosine (5-MeC)-DNA glycosylase needs both protein and RNA. RNA from enzyme purified by SDS-PAGE was isolated and cloned. The clones have an insert ranging from 240 to 670 bp an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e193b46755d4f300c047cae228cfe101
http://doc.rero.ch/record/304069/files/25-22-4545.pdf
http://doc.rero.ch/record/304069/files/25-22-4545.pdf
Publikováno v:
Solvent Extraction and Ion Exchange. 25:257-271
We present a molecular dynamics study of concentrated solutions of K+⊂222 cryptates, comparing two counterions X− (Chloride Cl− versus Picrate Pic−) and three solvents (water, acetonitrile, and chloroform), with the main aim to investigate th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::78737accde76abc209751f4cf8ec6476
https://doi.org/10.1080/07366290601169451
https://doi.org/10.1080/07366290601169451
Publikováno v:
European Journal of Biochemistry. 112:443-450
The messenger RNA coding for chicken vitellogenin, a precursor of the egg-yolk proteins lipovitellin and phosvitin, is synthesized in the liver following estrogen injection. This mRNA is 6600 nucleotides long. We have previously reported the cloning
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eacec18dd7d544a51991e1b4cfd40d6d
https://doi.org/10.1111/j.1432-1033.1980.tb06106.x
https://doi.org/10.1111/j.1432-1033.1980.tb06106.x
Publikováno v:
Supramolecular Chemistry. 15:133-142
(2003). Interfacial Activity of the Diprotonated 222 Cryptand at the Water/'Oil' Interface Revealed by Molecular Dynamics Simulations. Supramolecular Chemistry: Vol. 15, No. 2, pp. 133-142.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8090cf0283e88552bb21ff1b05fd54f3
https://doi.org/10.1080/1061027021000041456
https://doi.org/10.1080/1061027021000041456
Publikováno v:
FEBS Letters. 519:128-134
Human mature sperm cells have a high nuclease and 5-methyldeoxycytidine monophosphate (5-mdCMP) deaminase activity. The deaminase converts the nuclease degradation product 5-mdCMP into dTMP which is further cleaved into thymine and the abasic sugar-p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18eb07e2e28ff906774c2855c3da57dc
https://doi.org/10.1016/s0014-5793(02)02737-0
https://doi.org/10.1016/s0014-5793(02)02737-0
Autor:
Don Benjamin, Jean-Pierre Jost
Publikováno v:
Nucleic Acids Research. 29:3603-3610
We have constructed a stable cell line, human embryonal kidney 293M+, containing a lacZ reporter gene controlled by an in vitro methylated hormone-responsive enhancer. Methylation of the enhancer–promoter abolishes lacZ expression controlled by pon
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67c05fee9f6f9d2d23b9755fbbecad3c
https://doi.org/10.1093/nar/29.17.3603
https://doi.org/10.1093/nar/29.17.3603
Autor:
Yong Zheng, Stéphane Thiry, Michel Siegmann, Bing Zhu, Jean-Pierre Jost, Herbert Angliker, Don Benjamin
Publikováno v:
Proceedings of the National Academy of Sciences. 98:5031-5036
We have shown that the DNA demethylation complex isolated from chicken embryos has a G⋅T mismatch DNA glycosylase that also possesses 5-methylcytosine DNA glycosylase (5-MCDG) activity. Herein we show that human embryonic kidney cells stably transf
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b94bb3bd0d7d12ccccb9124b084744e
https://doi.org/10.1073/pnas.091097298
https://doi.org/10.1073/pnas.091097298