Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Piergiuseppe Nestola"'
Autor:
Sónia Mendes, Tiago Q. Faria, André Nascimento, Marc Noverraz, Franziska Bollmann, Piergiuseppe Nestola, António Roldão, Cristina Peixoto, Ricardo J. S. Silva
Publikováno v:
Separations, Vol 11, Iss 3, p 73 (2024)
Adeno-associated viruses (AAV) are currently predominant viral transfer tools for gene therapy, and efforts are being made to design faster and more efficient methods and technologies for their manufacturing. The early selection of high-performing fi
Externí odkaz:
https://doaj.org/article/6cdce523228341529b56a6e926a4ca43
Autor:
Piergiuseppe Nestola, Duarte L Martins, Cristina Peixoto, Susanne Roederstein, Tobias Schleuss, Paula M Alves, José P B Mota, Manuel J T Carrondo
Publikováno v:
PLoS ONE, Vol 9, Iss 12, p e115802 (2014)
The purification of virus particles and viral vectors for vaccine and gene therapy applications is gaining increasing importance in order to deliver a fast, efficient, and reliable production process. Ultrafiltration (UF) is a widely employed unit op
Externí odkaz:
https://doaj.org/article/b9968d7423bc44c083981427a051089b
Publikováno v:
Bioprocessing of Viral Vaccines ISBN: 9781003229797
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3654ed8d32d0a9e4780a4367581b5ce6
https://doi.org/10.1201/9781003229797-7
https://doi.org/10.1201/9781003229797-7
Autor:
Sarah C. Gilbert, Scott Bruce, Daniel C. Smith, Sarah Davies, Carina Citra Dewi Joe, Adam Berg, Adam J. Ritchie, Daniel Pappas, Yuanyuan Li, Piergiuseppe Nestola, Maria Kapanidou, Amar Joshi, Paul Cashen, Gaurav Gupta, Raghavan Venkat, Alexander D. Douglas, Lee Davies, David O. Mainwaring, Mark Woodyer, Richard Turner, Daan Bormans, Jonathan Humphreys, Sofiya Fedosyuk, Catherine M. Green, Nick Smith, Dennis Verbart, Nitin Chopra, Nicole Bleckwenn, Teresa Lambe, Jinlin Jiang, Rameswara R Segireddy, Thomas Linke, Lisa Cooper, Byron Rees, Carol Knevelman
Publikováno v:
Biotechnology and Bioengineering
Manufacturing has been the key factor limiting rollout of vaccination during the COVID‐19 pandemic, requiring rapid development and large‐scale implementation of novel manufacturing technologies. ChAdOx1 nCoV‐19 (AZD1222, Vaxzevria) is an effic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78020b799701a9645d14a6b2ea46dd38
https://ora.ox.ac.uk/objects/uuid:9aab02ba-dac8-40f5-979a-f773c0fbf98d
https://ora.ox.ac.uk/objects/uuid:9aab02ba-dac8-40f5-979a-f773c0fbf98d
Autor:
Carina C D Joe, Rameswara R Segireddy, Cathy Oliveira, Adam Berg, Yuanyuan Li, Dimitrios Doultsinos, Nitin Chopra, Steffi Scholze, Asma Ahmad, Piergiuseppe Nestola, Julia Niemann, Alexander D Douglas
The Coalition for Epidemic Preparedness Innovations’ ‘100-day moonshot’ aspires to launch a new vaccine within 100 days of pathogen identification. Here, we describe work to optimize adenovirus vector manufacturing for rapid response, by minimi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::45a745fc08c6cd9e95a4fb79670ad183
https://doi.org/10.1101/2021.12.22.473478
https://doi.org/10.1101/2021.12.22.473478
Autor:
José P. B. Mota, Paula M. Alves, Piergiuseppe Nestola, Cristina Peixoto, Manuel J.T. Carrondo, Ricardo J.S. Silva
Publikováno v:
Biotechnology and Bioengineering. 112:843-857
The downstream processing of virus particles for vaccination or gene therapy is becoming a critical bottleneck as upstream titers keep improving. Moreover, the growing pressure to develop cost-efficient processes has brought forward new downstream tr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3223e0599746eb320d109bec4115aad2
https://doi.org/10.1002/bit.25545
https://doi.org/10.1002/bit.25545
Autor:
Louis Villain, Paula M. Alves, Piergiuseppe Nestola, Cristina Peixoto, Manuel J.T. Carrondo, José P. B. Mota
Publikováno v:
Journal of chromatography. A. 1426
We report on the rational design and implementation of flowthrough (FT) platforms for purification of virus vectors (VVs) and virus-like particles (VLPs), combining anion-exchange polyallylamine membranes (Sartobind STIC) and core-shell octylamine re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dde15d280192643790e7b4923afb37d3
https://pubmed.ncbi.nlm.nih.gov/26643723
https://pubmed.ncbi.nlm.nih.gov/26643723
Autor:
Paula M. Alves, Manuel J.T. Carrondo, José P. B. Mota, Cristina Peixoto, Piergiuseppe Nestola, Ricardo J.S. Silva
Publikováno v:
Journal of biotechnology. 213
A simple, yet efficient, two-column simulated moving-bed (2CSMB) process for purifying adenovirus serotype 5 (Ad5) by size-exclusion chromatography (SEC) is presented and validated experimentally, and a general procedure for its robust design under p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23af6b7396d88f10ebe60f590c67e0a6
https://pubmed.ncbi.nlm.nih.gov/25744665
https://pubmed.ncbi.nlm.nih.gov/25744665
Autor:
Ricardo J.S. Silva, Piergiuseppe Nestola, Cristina Peixoto, José P. B. Mota, Paula M. Alves, Manuel J.T. Carrondo
Publikováno v:
Journal of chromatography. A. 1347
Adenovirus serotype 5 (Ad5) was successfully separated by size-exclusion chromatography (SEC) using a simple, yet efficient, two-column, quasi-continuous, simulated moving-bed process operated in an open-loop configuration. The operating cycle is div
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c048417b95b66c761bb9ff4fc65ffa90
https://pubmed.ncbi.nlm.nih.gov/24813933
https://pubmed.ncbi.nlm.nih.gov/24813933
Autor:
Paula M. Alves, Tobias Schleuss, José P. B. Mota, Cristina Peixoto, Susanne Roederstein, D. Martins, Piergiuseppe Nestola, Manuel J.T. Carrondo
Publikováno v:
PLoS ONE, Vol 9, Iss 12, p e115802 (2014)
PLoS ONE
PLoS ONE
The purification of virus particles and viral vectors for vaccine and gene therapy applications is gaining increasing importance in order to deliver a fast, efficient, and reliable production process. Ultrafiltration (UF) is a widely employed unit op
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a60c700dd235c2c5e6e39978c9b85219
http://europepmc.org/articles/PMC4278829?pdf=render
http://europepmc.org/articles/PMC4278829?pdf=render