Výsledky vyhledávání - "Phylicia Kidd"

Unraveling cellular complexity with unlimited multiplexed super-resolution imaging

Autor: Florian Schueder, Felix Rivera-Molina, Maohan Su, Phylicia Kidd, James E. Rothman, Derek Toomre, Joerg Bewersdorf

SummaryMapping the intricate spatial relationships between the many different molecules inside a cell is essential to understanding cellular functions in all their complexity. Super-resolution fluorescence microscopy offers the required spatial resol

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::aa36da857f21294ba1d3182630c2ed4e
https://doi.org/10.1101/2023.05.17.541061

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An integrated platform for high-throughput nanoscopy

Autor: Andrew E S Barentine, Yu Lin, Edward M Courvan, Phylicia Kidd, Miao Liu, Leonhard Balduf, Timy Phan, Felix Rivera-Molina, Michael R Grace, Zach Marin, Mark Lessard, Juliana Rios Chen, Siyuan Wang, Karla M Neugebauer, Joerg Bewersdorf, David Baddeley

Publikováno v: Nature Biotechnology.

Diffraction-unlimited single-molecule techniques like STORM and (F)PALM enable three-dimensional (3D) fluorescence imaging at tens of nanometer resolution and are invaluable to investigate sub-cellular organization. The multitude of camera frames req

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6cdd0517d4be9044eaab9ffc37a50ce5
https://doi.org/10.1038/s41587-023-01702-1

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Fluorogenic DNA-PAINT for faster, low-background super-resolution imaging

Autor: Kenny K. H. Chung, Zhao Zhang, Phylicia Kidd, Yongdeng Zhang, Nathan D. Williams, Bennett Rollins, Yang Yang, Chenxiang Lin, David Baddeley, Joerg Bewersdorf

Publikováno v: Nature Methods. 19:554-559

DNA-based points accumulation for imaging in nanoscale topography (DNA-PAINT) is a powerful super-resolution microscopy method that can acquire high-fidelity images at nanometer resolution. It suffers, however, from high background and slow imaging s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a71dbc882047a8e99b75b661c1c54233
https://doi.org/10.1038/s41592-022-01464-9

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Extremely Bright, Near-IR Emitting Spontaneously Blinking Fluorophores Enable Ratiometric Multicolor Nanoscopy in Live Cells

Autor: Alanna Schepartz, Jonathan Tyson, Neville Dadina, Ling Chu, Kevin Hu, Derek Toomre, Phylicia Kidd, Joerg Bewersdorf, Shuai Zheng

Publikováno v: ACS Central Science, Vol 7, Iss 8, Pp 1419-1426 (2021)
ACS central science, vol 7, iss 8
ACS Central Science

New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule localization microscopy (SMLM). Here we report the design, synthesis, and biological evaluation o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c304744ef0072eb9f842b83cda301ba
https://doi.org/10.1021/acscentsci.1c00670

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All-optical visualization of specific molecules in the ultrastructural context of brain tissue

Autor: Ons M’Saad, Ravikiran Kasula, Ilona Kondratiuk, Phylicia Kidd, Hanieh Falahati, Juliana E. Gentile, Robert F. Niescier, Katherine Watters, Robert C. Sterner, Seong Lee, Xinran Liu, Pietro De Camilli, James E. Rothman, Anthony J. Koleske, Thomas Biederer, Joerg Bewersdorf

SummaryUnderstanding the molecular anatomy and neural connectivity of the brain requires imaging technologies that can map the 3D nanoscale distribution of specific proteins in the context of brain ultrastructure. Light and electron microscopy (EM) e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::74e08273d1a251299cca52bae87aa56b
https://doi.org/10.1101/2022.04.04.486901

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Three-dimensional adaptive optical nanoscopy for thick specimen imaging at sub-50-nm resolution

Autor: Lynn Cooley, Thomas Biederer, Francesca Bottanelli, Dong-Ryoung Lee, Mark D. Lessard, Jacopo Antonello, Martin J. Booth, Katherine Watters, Lena K. Schroeder, Phylicia Kidd, Julianne A. Gerdes, Xiang Hao, Joerg Bewersdorf, Jiaxi Zhao, James E. Rothman, Edward S. Allgeyer

Publikováno v: Nature Methods
Nature methods, vol 18, iss 6
Nature methods

Understanding cellular organization demands the best possible spatial resolution in all three dimensions. In fluorescence microscopy, this is achieved by 4Pi nanoscopy methods that combine the concepts of using two opposing objectives for optimal dif

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4f6a1aff96912ffc6dc531e04ed313b

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3D super-resolution deep-tissue imaging in living mice

Autor: Martin J. Booth, Valentina Greco, Mary Grace M. Velasco, Jacopo Antonello, Edward S. Allgeyer, Dennis May, Ons M’Saad, Andrew E.S. Barentine, Peng Yuan, Jaime Grutzendler, Joerg Bewersdorf, Mengyang Zhang, Phylicia Kidd

Publikováno v: Optica
bioRxiv

Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering unique insights into their organization. However, 3D-STED imaging deep inside biological tissue

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62c0d0b167e7128fb12ec0636da4363e
https://pubmed.ncbi.nlm.nih.gov/34239948

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3D Adaptive Optical Nanoscopy for Thick Specimen Imaging at sub-50 nm Resolution

Autor: Jacopo Antonello, Martin J. Booth, Lena K. Schroeder, Francesca Bottanelli, Joerg Bewersdorf, Xiang Hao, Phylicia Kidd, Edward S. Allgeyer, Lynn Cooley, Julianne A. Gerdes, Thomas Biederer, Jiaxi Zhao, James E. Rothman, Katherine Watters, Mark D. Lessard

Understanding cellular organization demands the best possible spatial resolution in all three dimensions (3D). In fluorescence microscopy, this is achieved by 4Pi nanoscopy methods that combine the concepts of using two opposing objectives for optima

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::fec245cdcac6500073e46bfcae924f5c
https://doi.org/10.1101/2020.11.25.398958

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