Zobrazeno 1 - 10
of 189
pro vyhledávání: '"Phillip D, Zamore"'
Publikováno v:
STAR Protocols, Vol 4, Iss 2, Pp 102336- (2023)
Summary: Binding affinity quantitatively describes the strength of a molecular interaction and is reported by the equilibrium dissociation constant (KD). Here, we present a protocol to measure KD of mammalian microRNA-loaded Argonaute2 protein by dou
Externí odkaz:
https://doaj.org/article/6d1f7c2a85a04687ac738e1d29345a05
Autor:
Tianxiong Yu, Kaili Fan, Deniz M. Özata, Gen Zhang, Yu Fu, William E. Theurkauf, Phillip D. Zamore, Zhiping Weng
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)
The pachytene piRNA loci are transcribed by RNA polymerase II in the male germline of placental mammals. Here the authors show that a long first exon or a long unspliced transcript correlates with germline-specific production of piRNA precursor trans
Externí odkaz:
https://doaj.org/article/1760126de9e147de89acee694ee12b89
Autor:
Carlas S. Smith, Karina Jouravleva, Maximiliaan Huisman, Samson M. Jolly, Phillip D. Zamore, David Grunwald
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-9 (2019)
Analysis of single-molecule binding assays still requires substantial manual user intervention. Here, the authors present a pipeline for rapid, automated analysis of co-localization single-molecule spectroscopy images, with a modular user interface t
Externí odkaz:
https://doaj.org/article/843e839540ba4dd28ca6c75bbc68d526
Publikováno v:
BMC Genomics, Vol 19, Iss 1, Pp 1-14 (2018)
Abstract Background RNA-seq and small RNA-seq are powerful, quantitative tools to study gene regulation and function. Common high-throughput sequencing methods rely on polymerase chain reaction (PCR) to expand the starting material, but not every mol
Externí odkaz:
https://doaj.org/article/98cfcae444e64eac8d2cedd188d8344a
Autor:
Yi Lu, Phillip W.L. Tai, Jianzhong Ai, Dominic J. Gessler, Qin Su, Xieyi Yao, Qiang Zheng, Phillip D. Zamore, Xun Xu, Guangping Gao
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 10, Iss , Pp 349-360 (2018)
Corneal neovascularization (NV) is the major sight-threatening pathology caused by angiogenic stimuli. Current drugs that directly target pro-angiogenic factors to inhibit or reverse the disease require multiple rounds of administration and have limi
Externí odkaz:
https://doaj.org/article/c477d019d31e4780b69ad117ac5bd918
Autor:
Sukanya Iyer, Aamir Mir, Joel Vega-Badillo, Benjamin P. Roscoe, Raed Ibraheim, Lihua Julie Zhu, Jooyoung Lee, Pengpeng Liu, Kevin Luk, Esther Mintzer, Dongsheng Guo, Josias Soares de Brito, Charles P. Emerson, Phillip D. Zamore, Erik J. Sontheimer, Scot A. Wolfe
Publikováno v:
The CRISPR Journal. 5:685-701
While genome editing has been revolutionized by the advent of CRISPR-based nucleases, difficulties in achieving efficient, nuclease-mediated, homology-directed repair (HDR) still limit many applications. Commonly used DNA donors such as plasmids suff
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2676939cee7f1aebba005624d2b76eb9
https://doi.org/10.1089/crispr.2022.0058
https://doi.org/10.1089/crispr.2022.0058
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
Abstract Eubacterial ribosomal large-subunit methyltransferase H (RlmH) methylates 23S ribosomal RNA pseudouridine 1915 (Ψ1915), which lies near the ribosomal decoding center. The smallest member of the SPOUT superfamily of methyltransferases, RlmH
Externí odkaz:
https://doaj.org/article/d054ded6d21e412c86951938f4ea5fff
Autor:
Amena Arif, Shannon Bailey, Natsuko Izumi, Todd A. Anzelon, Deniz M. Ozata, Cecilia Andersson, Ildar Gainetdinov, Ian J. MacRae, Yukihide Tomari, Phillip D. Zamore
Publikováno v:
Nature. 608:618-625
Argonaute proteins use nucleic acid guides to find and bind specific DNA or RNA target sequences. Argonaute proteins have diverse biological functions and many retain their ancestral endoribonuclease activity, cleaving the phosphodiester bond between
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::703fb3283d992e25866ef0b761130585
https://doi.org/10.1038/s41586-022-05009-0
https://doi.org/10.1038/s41586-022-05009-0
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 6, Iss 10, Pp 3197-3206 (2016)
Adoption of a streamlined version of the bacterial clustered regular interspersed short palindromic repeat (CRISPR)/Cas9 defense system has accelerated targeted genome engineering. The Streptococcus pyogenes Cas9 protein, directed by a simplified, CR
Externí odkaz:
https://doaj.org/article/ca144083ff2a422cb941669ca1370127
Autor:
Yu Fu, Yujing Yang, Han Zhang, Gwen Farley, Junling Wang, Kaycee A Quarles, Zhiping Weng, Phillip D Zamore
Publikováno v:
eLife, Vol 7 (2018)
We report a draft assembly of the genome of Hi5 cells from the lepidopteran insect pest, Trichoplusia ni, assigning 90.6% of bases to one of 28 chromosomes and predicting 14,037 protein-coding genes. Chemoreception and detoxification gene families re
Externí odkaz:
https://doaj.org/article/ace83d72deb04817b8e59d1186487194