Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Phillip A Rachwal"'
Autor:
Phillip A Rachwal, Helen L Rose, Victoria Cox, Roman A Lukaszewski, Amber L Murch, Simon A Weller
Publikováno v:
PLoS ONE, Vol 7, Iss 4, p e35971 (2012)
The TaqMan Array Card architecture, normally used for gene expression studies, was evaluated for its potential to detect multiple bacterial agents by real-time PCR. Ten PCR assays targeting five biological agents (Bacillus anthracis, Burkholderia mal
Externí odkaz:
https://doaj.org/article/26cbadfaf983410b96e55425f883a4e4
Autor:
Adrienne T. Hall, Jeffrey W. Koehler, Phillip A. Rachwal, Simon A. Weller, Timothy D. Minogue
Publikováno v:
Applied and Environmental Microbiology. 80:1322-1329
Rapid pathogen detection is crucial for the timely introduction of therapeutics. Two groups (one in the United Kingdom and one in the United States) independently evaluated inhibitor-resistant PCR reagents for the direct testing of substrates. In the
G-Rich sequences are known to form four-stranded structures that are based on stacks of G-quartets, and sequences with the potential to adopt these structures are common in eukaryotic genomes. However, there are few rules for predicting the relative
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59b7349a8dc08eab5e0ea9473f02b428
https://ora.ox.ac.uk/objects/uuid:13f547f2-2eee-4384-9957-e633f525cf4f
https://ora.ox.ac.uk/objects/uuid:13f547f2-2eee-4384-9957-e633f525cf4f
Autor:
Abigael Kosgey, Jasmine Portmann, Giovanna Jaramillo Gutierrez, Raymond Pallawo, Martin Gabriel, Lauren A. Cowley, Babak Afrough, Amadou Bah, Emma Hutley, Andrew Johnston, Phillip A. Rachwal, Antonio Mazzarelli, Boubacar Diallo, Ettore Severi, Kilian Stoecker, Marianne Gerard, Jonathan H.J. Baum, Amy Mikhail, Sarah Meisel, Koumpingnin Yacouba Nebie, Jared T. Simpson, Juliane Doerrbecker, Nobila Ouedraogo, Mar Cabeza-Cabrerizo, Abdoulaye Camara, Erna Fleischmann, Georgios Pollakis, Nicholas J. Loman, Gytis Dudas, Natacha Milhano, Erasmus Smit, Christopher H. Logue, Roman Wölfel, Cecelia V. Williams, Ousmane Faye, Michel Koropogui, Stephan Günther, Jon Gerlach, Frank Washington, Kuiama Lewandowski, Bernard Baillet, Beate Becker-Ziaja, Livia Victoria Patrono, Andreas Sachse, Abdoulaye Diarra, David A. Matthews, Janine Michel, Álvaro Camino-Sánchez, Lamine Koivogui, Rahel L. Yemanaberhan, Oumar Faye, Sophie Duraffour, Jan Peter Boettcher, Theresa Enkirch, Matthew K. O'Shea, Vanessa Monteil, Marion Bererd, Katja Nitzsche, Linda Winona, Miles W. Carroll, Hermann Somlare, Amadou A. Sall, Natasha Y. Rickett, James E. Taylor, Pierre Formenty, Alimou Camara, Antonino Di Caro, Joseph Akoi Bore, Julian A. Hiscox, Daniel J. Turner, Nicole Hetzelt, Julia Hinzmann, Isabelle Roger, Duncan W. Wilson, Joshua Quick, Trina Racine, Emily Davis, Guillaume Bado, Katrin Singethan, Déborah Delaune, Elsa Gayle Zekeng, Raymond Koundouno, Isabel García-Dorival, Yacouba Savane, Alexander Bello, Victoria Amburgey, Inês Vitoriano, Marc Mertens, Lisa L. Carter, Liana E. Kafetzopoulou, N’Faly Magassouba, Tobias Holm, Didier Ngabo, Andrew Rambaut, Simon A. Weller, Eeva Kuisma, Marine Jourdain, Facinet Yattara, Christopher Williams, Sakoba Keita, Johanna Repits, Elisa Pallasch
Publikováno v:
Nature
Sygma
Europe PubMed Central
Sygma
Europe PubMed Central
A nanopore DNA sequencer is used for real-time genomic surveillance of the Ebola virus epidemic in the field in Guinea; the authors demonstrate that it is possible to pack a genomic surveillance laboratory in a suitcase and transport it to the field
Publikováno v:
Biophysical Chemistry. 145:105-110
We have studied the formation of DNA triple helices in different sequence contexts and show that, for the most stable triplexes, their apparent stability is affected by the stability of the underlying duplex. For a 14-mer parallel triplex-forming oli
Autor:
Phillip A, Rachwal, Keith R, Fox
Publikováno v:
Methods. 43:291-301
Melting curves are commonly used to determine the stability of folded nucleic acid structures and their interaction with ligands. This paper describes how the technique can be applied to study the properties of four-stranded nucleic acid structures t
Publikováno v:
FEBS Letters. 581:1657-1660
We have examined the properties of intramolecular G-quadruplexes in which the G3 tracts are separated by single base loops. The most stable complex contained 1',2'-dideoxyribose in all three loops, while loops containing T and C were slightly less st
Publikováno v:
Current microbiology. 68(4)
Transposon-directed insertion site sequencing was used to identify genes required by Bacillus thuringiensis to survive in non-axenic plant/soil microcosms. A total of 516 genetic loci fulfilled the criteria as conferring survival characteristics. Of
Autor:
Alistair H. Bishop, Phillip A. Rachwal
Publikováno v:
Current microbiology. 68(6)
Transposon-directed insertion site sequencing was used to identify genes required by Burkholderia thailandensis to survive in plant/soil microcosms. A total of 1,153 genetic loci fulfilled the criteria as being likely to encode survival characteristi
Autor:
Roman A. Lukaszewski, Victoria Cox, Angela E. Essex-Lopresti, Simon A. Weller, Margaret G. Hartley, Tanya M. Parsons, Phillip A. Rachwal, Helen L. Stapleton
Publikováno v:
Journal of Bioterrorism & Biodefense.
Three real-time PCR assays to detect Bacillus anthracis genetic targets (pXO1; pXO2 and chromosome) were developed. Two of the PCR assays (pXO1-MGB and Ba chr-MGB) were tested against DNA extracts produced from whole blood samples obtained from a rep