Zobrazeno 1 - 10
of 33
pro vyhledávání: '"Philippe V, Jutras"'
Autor:
Gail M. Preston, Philippe V. Jutras, Isobel Dodds, Mariana Schuster, Renier A. L. van der Hoorn, Riccardo Soldan
Publikováno v:
The Plant Journal. 108:600-612
Agroinfiltration in Nicotiana benthamiana is widely used to transiently express heterologous proteins in plants. However, the state of Agrobacterium itself is not well studied in agroinfiltrated tissues, despite frequent studies of immunity genes con
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2480
Bioluminescence enables the monitoring of spatiotemporal dynamics and activity of bacterial populations in planta. We here describe a procedure to use AgroLux, a bioluminescent Agrobacterium tumefaciens, as a tool to study bacterial responses upon ag
Autor:
Frank eSainsbury, Philippe V. Jutras, Juan eVorster, Marie-Claire eGoulet, Dominique eMichaud
Publikováno v:
Frontiers in Plant Science, Vol 7 (2016)
The use of plants as expression hosts for recombinant proteins is an increasingly attractive option for the production of complex and challenging biopharmaceuticals. Tools are needed at present to marry recent developments in high-yielding gene vecto
Externí odkaz:
https://doaj.org/article/6b2ed584e9654a109e52ff1600c5e4f1
Autor:
Philippe V Jutras, Carla Marusic, Chiara Lonoce, Carole Deflers, Marie-Claire Goulet, Eugenio Benvenuto, Dominique Michaud, Marcello Donini
Publikováno v:
PLoS ONE, Vol 11, Iss 11, p e0167086 (2016)
The overall quality of recombinant IgG antibodies in plants is dramatically compromised by host endogenous proteases. Different approaches have been developed to reduce the impact of endogenous proteolysis on IgGs, notably involving site-directed mut
Externí odkaz:
https://doaj.org/article/29d2ff07321b4003b813b47f345eb40f
Publikováno v:
Recombinant Proteins in Plants ISBN: 9781071622407
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::32f4e4cd6a36a57414dd126910d6e2cb
https://doi.org/10.1007/978-1-0716-2241-4_15
https://doi.org/10.1007/978-1-0716-2241-4_15
Autor:
Rachel Tardif, Marie-Claire Goulet, Pierre-Olivier Lavoie, Frank Sainsbury, Louis-Philippe Hamel, Marc-André D'Aoust, Philippe V. Jutras, Dominique Michaud
Publikováno v:
Journal of Proteome Research. 19:106-118
Partial neutralization of the Golgi lumen pH by the ectopic expression of influenza virus M2 proton channel is useful to stabilize acid-labile recombinant proteins in plant cells, but the impact of pH gradient mitigation on host cellular functions ha
Autor:
Philippe V, Jutras, Frank, Sainsbury, Marie-Claire, Goulet, Pierre-Olivier, Lavoie, Rachel, Tardif, Louis-Philippe, Hamel, Marc-André, D'Aoust, Dominique, Michaud
Publikováno v:
Journal of proteome research. 19(1)
Partial neutralization of the Golgi lumen pH by the ectopic expression of influenza virus M2 proton channel is useful to stabilize acid-labile recombinant proteins in plant cells, but the impact of pH gradient mitigation on host cellular functions ha
Molecular farming increasingly uses the tobacco relative Nicotiana benthamiana for production of recombinant proteins through transient expression. Several proteins are produced efficiently with this expression platform, but yields for other proteins
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c77d3608d511a7e6d95f3b4b670d861
https://zenodo.org/record/3970435
https://zenodo.org/record/3970435
Autor:
Garry Morgan, Frank Sainsbury, Philippe V. Jutras, Eva C. Thuenemann, Michael J. Landsberg, Noor H. Dashti, George P. Lomonossoff, Lou Brillault
Publikováno v:
ACS Nano. 11:3476-3484
Understanding capsid assembly following recombinant expression of viral structural proteins is critical to the design and modification of virus-like nanoparticles for biomedical and nanotechnology applications. Here, we use plant-based transient expr
Autor:
Philippe V. Jutras, Friederike M. Grosse-Holz, Markus Kaiser, Renier A. L. van der Hoorn, Dominique Michaud, Farnusch Kaschani
Publikováno v:
Plant Biotechnology Journal
Co-expression of protease inhibitors like the tomato cystatin SlCYS8 is useful to increase recombinant protein production in plants, but key proteases involved in protein proteolysis are still unknown. Here, we performed activity-based protein profil