Výsledky vyhledávání - "Philippe E. Mangeot"

Akademický článek

Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins

Autor: Philippe E. Mangeot, Valérie Risson, Floriane Fusil, Aline Marnef, Emilie Laurent, Juliana Blin, Virginie Mournetas, Emmanuelle Massouridès, Thibault J. M. Sohier, Antoine Corbin, Fabien Aubé, Marie Teixeira, Christian Pinset, Laurent Schaeffer, Gaëlle Legube, François-Loïc Cosset, Els Verhoeyen, Théophile Ohlmann, Emiliano P. Ricci

Publikováno v: Nature Communications, Vol 10, Iss 1, Pp 1-15 (2019)

A current challenge in genome editing is delivering Cas9 and sgRNA into target cells. Here the authors engineer a delivery system based on murine leukemia virus-like particles loaded with Cas9-sgRNA ribonucleoproteins to induce efficient genome editi

Externí odkaz: https://doaj.org/article/0bd13889c5db4e0ba26c33892287ae45

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Akademický článek

Baboon Envelope Pseudotyped 'Nanoblades' Carrying Cas9/gRNA Complexes Allow Efficient Genome Editing in Human T, B, and CD34+ Cells and Knock-in of AAV6-Encoded Donor DNA in CD34+ Cells

Autor: Alejandra Gutierrez-Guerrero, Maria Jimena Abrey Recalde, Philippe E. Mangeot, Caroline Costa, Ornellie Bernadin, Séverine Périan, Floriane Fusil, Gisèle Froment, Adriana Martinez-Turtos, Adrien Krug, Francisco Martin, Karim Benabdellah, Emiliano P. Ricci, Simone Giovannozzi, Rik Gijsbers, Eduard Ayuso, François-Loïc Cosset, Els Verhoeyen

Publikováno v: Frontiers in Genome Editing, Vol 3 (2021)

Programmable nucleases have enabled rapid and accessible genome engineering in eukaryotic cells and living organisms. However, their delivery into human blood cells can be challenging. Here, we have utilized “nanoblades,” a new technology that de

Externí odkaz: https://doaj.org/article/1d5485f02cd64cd39b1ee599b28883bf

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Akademický článek

A Versatile Strategy to Reduce UGA-Selenocysteine Recoding Efficiency of the Ribosome Using CRISPR-Cas9-Viral-Like-Particles Targeting Selenocysteine-tRNA[Ser]Sec Gene

Autor: Caroline Vindry, Olivia Guillin, Philippe E. Mangeot, Théophile Ohlmann, Laurent Chavatte

Publikováno v: Cells, Vol 8, Iss 6, p 574 (2019)

The translation of selenoprotein mRNAs involves a non-canonical ribosomal event in which an in-frame UGA is recoded as a selenocysteine (Sec) codon instead of being read as a stop codon. The recoding machinery is centered around two dedicated RNA com

Externí odkaz: https://doaj.org/article/b4f223641178404fa72f0ad99576a24b

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A homozygous mutation in the human selenocysteine tRNA gene impairs UGA recoding activity and selenoproteome regulation by selenium

Autor: Caroline Vindry, Olivia Guillin, Philippe Wolff, Paul Marie, Franck Mortreux, Philippe E Mangeot, Théophile Ohlmann, Laurent Chavatte

Publikováno v: Nucleic Acids Research
Nucleic Acids Research, 2023, ⟨10.1093/nar/gkad482⟩

The selenocysteine (Sec) tRNA (tRNA[Ser]Sec) governs Sec insertion into selenoproteins by the recoding of a UGA codon, typically used as a stop codon. A homozygous point mutation (C65G) in the human tRNA[Ser]Sec acceptor arm has been reported by two

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d63539fc597931f08e32ae5f0318ae7f
https://hal.science/hal-04136959/document

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Nanoblades allow high-level genome editing in organoids

Autor: Victor Tirolle, Adrien Krug, Emma Bokobza, Mattijs Bulcaen, Marjolein M. Ensinck, Maarten H. Geurts, Delilah Hendriks, François Vermeulen, Frédéric Larbret, Alejandra Gutierrez-Guerrero, Louise Medaer, Rik Gijsbers, Philippe E. Mangeot, Hans Clevers, Marianne S. Carlon, Frederic Bost, Els Verhoeyen

Genome engineering has become more accessible thanks to the RNA programmable endonucleases such as the CRISPR/Cas9 system. However, using this editing technology in synthetic organs called ‘organoids’ is still very inefficient. This is due to the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::bd862d8b5bbe9ad027009690e7538a3b
https://doi.org/10.1101/2022.08.04.502859

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Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles ('Nanoblades')

Autor: Philippe E, Mangeot, Laura, Guiguettaz, Thibault J M, Sohier, Emiliano P, Ricci

Publikováno v: Journal of visualized experiments : JoVE. (169)

The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system has democratized genome-editing in eukaryotic cells and led to the development of numerous innovative applications. However, delivery of the Cas9 protein and single-gui

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::50ce44de1a2a107a556948457493f9d9
https://pubmed.ncbi.nlm.nih.gov/33871447

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Loop extrusion as a mechanism for formation of DNA damage repair foci

Autor: Gaëlle Legube, Thomas Clouaire, Vincent Rocher, Philippe E. Mangeot, Pierre Caron, Felix Zhou, Raphaël Mourad, James E. Haber, Coline Arnould, Daan Noordermeer, Kevin Li, Anne-Laure Finoux, Emiliano P. Ricci

Publikováno v: Nature
Nature, Nature Publishing Group, 2021, 590 (7847), pp.660-665. ⟨10.1038/s41586-021-03193-z⟩
Nature, 2021, 590 (7847), pp.660-665. ⟨10.1038/s41586-021-03193-z⟩

The repair of DNA double-strand breaks (DSBs) is essential for safeguarding genome integrity. When a DSB forms, the PI3K-related ATM kinase rapidly triggers the establishment of megabase-sized, chromatin domains decorated with phosphorylated histone

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ccd97d6413cb965ab452c3e3dff334b
https://hal.archives-ouvertes.fr/hal-03385752/document

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A single-chain and fast-responding light-inducible Cre recombinase as a novel optogenetic switch

Autor: Gaël Yvert, Théophile Ohlmann, Hélène Duplus-Bottin, Christophe Place, Gérard Triqueneaux, Philippe E. Mangeot, Martin Spichty, Franck Vittoz

Publikováno v: eLife, Vol 10 (2021)
eLife
eLife, 2021, 10, ⟨10.7554/eLife.61268⟩
eLife, eLife Sciences Publication, 2021, 10, ⟨10.7554/eLife.61268⟩

Optogenetics enables genome manipulations with high spatiotemporal resolution, opening exciting possibilities for fundamental and applied biological research. Here, we report the development of LiCre, a novel light-inducible Cre recombinase. LiCre is

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cab58b3e9bc419f4f12f4303e2f78968
https://doi.org/10.7554/elife.61268

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A monogenic and fast-responding Light-Inducible Cre recombinase as a novel optogenetic switch

Autor: Gaël Yvert, Hélène Duplus-Bottin, Franck Vittoz, Gérard Triqueneaux, Théophile Ohlmann, Christophe Place, Martin Spichty, Philippe E. Mangeot

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b33add42664329c39dffb8b044d17844
https://hal.science/hal-03055036/document

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