Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Philipp S. Spuhler"'
Autor:
Kyle C. Smith, John L. Walsh, Henry H. Hwang, Melissa Yu, Mehmet Toner, Sahil Shah, Fabio Fachin, Murat Karabacak, Shyamala Maheswaran, Daniel A. Haber, Ruby Yarmush, Ravi Kapur, Vincent Pai, Philipp S. Spuhler, Jennifer Yang, Joseph M. Martel-Foley, Jon F. Edd, Lecia V. Sequist, Shannon L. Stott, Tom Barber
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
Scientific Reports
Scientific Reports
Circulating tumor cells (CTCs) are a treasure trove of information regarding the location, type and stage of cancer and are being pursued as both a diagnostic target and a means of guiding personalized treatment. Most isolation technologies utilize p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::215e1644a4c992045d722542c86bf59d
https://doaj.org/article/451a9d3337944460bed973cc5aa9b39f
https://doaj.org/article/451a9d3337944460bed973cc5aa9b39f
Publikováno v:
Nanoscale. 8:5587-5598
DNA-binding proteins play crucial roles in the maintenance and functions of the genome and yet, their specific binding mechanisms are not fully understood. Recently, it was discovered that DNA-binding proteins recognize specific binding sites to carr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67a1a08c29700d3d57deefc7cefe9005
https://doi.org/10.1039/c5nr06785e
https://doi.org/10.1039/c5nr06785e
Autor:
Nikola Kojic, Kyle C. Smith, Shyamala Maheswaran, Vincent Pai, Bailey Morgan, Henry H. Hwang, Julie Trautwein, Fabio Fachin, Emre Ozkumur, Joseph M. Martel, Pin-i Chen, Philipp S. Spuhler, Shannon L. Stott, Tom Barber, Jennifer Yang, Ravi Kapur, Mehmet Toner, Eugene J. Lim, Daniel A. Haber, Nezihi Murat Karabacak
Publikováno v:
Nature Protocols. 9:694-710
The ability to isolate and analyze rare circulating tumor cells (CTCs) has the potential to further our understanding of cancer metastasis and enhance the care of cancer patients. In this protocol, we describe the procedure for isolating rare CTCs fr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aaf2564df3121970c78480e7374b2580
https://doi.org/10.1038/nprot.2014.044
https://doi.org/10.1038/nprot.2014.044
Autor:
Xirui Zhang, Marcella Chiari, Laura Sola, Philipp S. Spuhler, Joseph Greenspun, M. Selim Ünlü, Margo R. Monroe
Publikováno v:
Analytical chemistry (Wash.) 84 (2012): 10593–10599. doi:10.1021/ac3018263
info:cnr-pdr/source/autori:Spuhler, Philipp S.; Sola, Laura; Zhang, Xirui; Monroe, Margo R.; Greenspun, Joseph T.; Chiari, Marcella; Uenlue, M. Selim/titolo:Precisely Controlled Smart Polymer Scaffold for Nanoscale Manipulation of Biomolecules/doi:10.1021%2Fac3018263/rivista:Analytical chemistry (Wash.)/anno:2012/pagina_da:10593/pagina_a:10599/intervallo_pagine:10593–10599/volume:84
info:cnr-pdr/source/autori:Spuhler, Philipp S.; Sola, Laura; Zhang, Xirui; Monroe, Margo R.; Greenspun, Joseph T.; Chiari, Marcella; Uenlue, M. Selim/titolo:Precisely Controlled Smart Polymer Scaffold for Nanoscale Manipulation of Biomolecules/doi:10.1021%2Fac3018263/rivista:Analytical chemistry (Wash.)/anno:2012/pagina_da:10593/pagina_a:10599/intervallo_pagine:10593–10599/volume:84
We demonstrate the application of a novel smart surface to modulate the orientation of immobilized double stranded DNA (dsDNA) and the conformation of a polymer scaffold through variation in buffer pH and ionic strength. An amphoteric poly(dimethylac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f274fcf6553a449b278cf09411c3d12f
https://doi.org/10.1021/ac3018263
https://doi.org/10.1021/ac3018263
Autor:
Lidia Armelao, Francesco Damin, Philipp S. Spuhler, Marcella Chiari, Selim Unlu, Chiara Maccato, Gabriele Di Carlo
Publikováno v:
Applied surface science 258 (2012): 3750–3756. doi:10.1016/j.apsusc.2011.12.019
info:cnr-pdr/source/autori:Di Carlo G.; Damin F.; Armelao L.; MacCato C.; Unlu S.; Spuhler P.S.; Chiari M./titolo:Synthesis and conformational characterization of functional di-block copolymer brushes for microarray technology/doi:10.1016%2Fj.apsusc.2011.12.019/rivista:Applied surface science/anno:2012/pagina_da:3750/pagina_a:3756/intervallo_pagine:3750–3756/volume:258
info:cnr-pdr/source/autori:Di Carlo G.; Damin F.; Armelao L.; MacCato C.; Unlu S.; Spuhler P.S.; Chiari M./titolo:Synthesis and conformational characterization of functional di-block copolymer brushes for microarray technology/doi:10.1016%2Fj.apsusc.2011.12.019/rivista:Applied surface science/anno:2012/pagina_da:3750/pagina_a:3756/intervallo_pagine:3750–3756/volume:258
Surface initiated polymerization (SIP) coupled with reversible addition-fragmentation chain transfer polymerization (RAFT) was used to functionalize microarray glass slides with block polymer brushes. N,N-dimethylacrylamide (DMA) and N-acryloyloxysuc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81be68282cf9ce311d124ed52a4bde7b
https://doi.org/10.1016/j.apsusc.2011.12.019
https://doi.org/10.1016/j.apsusc.2011.12.019
Autor:
Xirui Zhang, Philipp S. Spuhler, Sunmin Ahn, M. Selim Ünlü, George G. Daaboul, David S. Freedman, Abdulkadir Yurt, Oguzhan Avci
Publikováno v:
The Analyst. 139(24)
Quantitative determination of the density and conformation of DNA molecules tethered to the surface can help optimize and understand DNA nanosensors and nanodevices, which use conformational or motional changes of surface-immobilized DNA for detectio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::915b579b628df07c7f3c1d0f833ffe19
https://pubmed.ncbi.nlm.nih.gov/25340741
https://pubmed.ncbi.nlm.nih.gov/25340741
Autor:
Mehmet Toner, Anya Kimura, Sudarshana Sengupta, Kyle C. Smith, David T. Ting, Ravi Kapur, Julie Trautwein, Nezihi Murat Karabacak, David N. Louis, Elena F. Brachtel, Jordan C. Ciciliano, Daniel A. Haber, Min Yu, Richard T. Lee, Shyamala Maheswaran, David T. Miyamoto, Tom Barber, Lecia V. Sequist, Shannon L. Stott, Aditya Bardia, Ajay Shah, John R. Walsh, Philipp S. Spuhler, James P. Sullivan, Bailey Morgan, Benjamin L. Emmink, Xi Luo, Alice T. Shaw, Emre Ozkumur, Pin-i Chen
Publikováno v:
Science Translational Medicine. 5
Circulating tumor cells (CTCs) are shed into the bloodstream from primary and metastatic tumor deposits. Their isolation and analysis hold great promise for the early detection of invasive cancer and the management of advanced disease, but technologi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10f3260c976f0af6857182047c657b94
https://doi.org/10.1126/scitranslmed.3005616
https://doi.org/10.1126/scitranslmed.3005616
Publikováno v:
Biosensors & bioelectronics 36 (2012): 222–229. doi:10.1016/j.bios.2012.04.020
info:cnr-pdr/source/autori:Ahn, Sunmin; Spuhler, Philipp S.; Chiari, Marcella; Cabodi, Mario; Uenlue, M. Selim/titolo:Quantification of surface etching by common buffers and implications on the accuracy of label-free biological assays/doi:10.1016%2Fj.bios.2012.04.020/rivista:Biosensors & bioelectronics/anno:2012/pagina_da:222/pagina_a:229/intervallo_pagine:222–229/volume:36
info:cnr-pdr/source/autori:Ahn, Sunmin; Spuhler, Philipp S.; Chiari, Marcella; Cabodi, Mario; Uenlue, M. Selim/titolo:Quantification of surface etching by common buffers and implications on the accuracy of label-free biological assays/doi:10.1016%2Fj.bios.2012.04.020/rivista:Biosensors & bioelectronics/anno:2012/pagina_da:222/pagina_a:229/intervallo_pagine:222–229/volume:36
High throughput analyses in biochemical assays are gaining popularity in the post-genomic era. Multiple label-free detection methods are especially of interest, as they allow quantitative monitoring of biomolecular interactions. It is assumed that th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14203b58c896a6419db2cbd61083a034
https://pubmed.ncbi.nlm.nih.gov/22560160
https://pubmed.ncbi.nlm.nih.gov/22560160
Autor:
Joseph Greenspun, Laura Sola, Xirui Zhang, Philipp S. Spuhler, Marcella Chiari, M. Selim Ünlü, Margo R. Monroe
Publikováno v:
16th International Conference on Optical MEMS and Nanophotonics.
We demonstrate controlled orientation of surface anchored DNA and utilize spectral self-interference fluorescent microscopy (SSFM) to measure fluorophore height with sub-nm precision to precisely quantify DNA orientation and conformation.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::92bf810934e77744836a69793e232e34
https://doi.org/10.1109/omems.2011.6031084
https://doi.org/10.1109/omems.2011.6031084