Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Philipp S Erdmann"'
Autor:
Sven Klumpe, Herman KH Fung, Sara K Goetz, Ievgeniia Zagoriy, Bernhard Hampoelz, Xiaojie Zhang, Philipp S Erdmann, Janina Baumbach, Christoph W Müller, Martin Beck, Jürgen M Plitzko, Julia Mahamid
Publikováno v:
eLife, Vol 10 (2021)
Lamella micromachining by focused ion beam milling at cryogenic temperature (cryo-FIB) has matured into a preparation method widely used for cellular cryo-electron tomography. Due to the limited ablation rates of low Ga+ ion beam currents required to
Externí odkaz:
https://doaj.org/article/dac8598bb5fe4b05b4258e93085e26ee
Autor:
Philipp S. Erdmann, Zhen Hou, Sven Klumpe, Sagar Khavnekar, Florian Beck, Florian Wilfling, Jürgen M. Plitzko, Wolfgang Baumeister
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-9 (2021)
The large and small subunits of the ribosome are synthesized independently within the nucleolus — a membrane-less compartment within the nucleus — before being exported into the cytoplasm. Here, the authors use in situ cryo-ET to observe ribosome
Externí odkaz:
https://doaj.org/article/29af8d2ce12c4b48a297291bce32bf53
Autor:
Leo E. Wong, Arshiya Bhatt, Philipp S. Erdmann, Zhen Hou, Joachim Maier, Sona Pirkuliyeva, Michael Engelke, Stefan Becker, Jürgen Plitzko, Jürgen Wienands, Christian Griesinger
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
Antibody-mediated immune responses rely on antigen recognition by the B cell antigen receptor (BCR) and SLP65 is a key scaffold protein mediating BCR signaling. Here authors show that effective B cell activation requires tripartite phase separation o
Externí odkaz:
https://doaj.org/article/274458207bae4608b1c142b4c80debc7
Autor:
Sven Klumpe, Jakub Kuba, Oda H. Schioetz, Philipp S. Erdmann, Alexander Rigort, Jürgen M. Plitzko
Publikováno v:
Microscopy Today. 30:42-47
Cryo-electron tomography (cryo-ET) enables visualization of protein complexes within their native cellular environment at molecular resolution. Most cells and all tissues, however, are too thick to be imaged directly by transmission electron microsco
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f0e2476980020b55f328602ff4d821ef
https://doi.org/10.1017/s1551929521001528
https://doi.org/10.1017/s1551929521001528
Autor:
Anna Bieber, Cristina Capitanio, Philipp S. Erdmann, Fabian Fiedler, Florian Beck, Chia-Wei Lee, Delong Li, Gerhard Hummer, Brenda A. Schulman, Wolfgang Baumeister, Florian Wilfling
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America
Autophagosomes are unique organelles that form de novo as double-membrane vesicles engulfing cytosolic material for destruction. Their biogenesis involves membrane transformations of distinctly shaped intermediates whose ultrastructure is poorly unde
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b00aac262a43a8b6e81934b20d57f6fc
https://hdl.handle.net/21.11116/0000-000B-1B0A-D
https://hdl.handle.net/21.11116/0000-000B-1B0A-D
Autor:
Sagar Khavnekar, Veronika Vrbovská, Magda Zaoralová, Ron Kelley, Florian Beck, Sven Klumpe, Abhay Kotecha, Jürgen Plitzko, Philipp S. Erdmann
We here present a method based on metallic platinum sputtering that can substantially enhance the quality of subtomogram averages from lamellas and thereby reduce the number of particles needed for high-resolution subtomogram averaging. We provide ev
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9b16c3caa7902447ba62e6395a175bd9
https://doi.org/10.1101/2022.06.16.496417
https://doi.org/10.1101/2022.06.16.496417
Autor:
Cristina Capitanio, Anna Bieber, Philipp S Erdmann, Brenda A Schulman, Wolfgang Baumeister, Florian Wilfling
This protocol describes how to plunge-freeze yeast on EM grids and how to target autophagic structures by combining cryo confocal fluorescence data to FIB-milling and tomogram acquisition
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::30b0e26e99b99f6a71b53f46dc5e2831
https://doi.org/10.17504/protocols.io.e6nvwkz4wvmk/v1
https://doi.org/10.17504/protocols.io.e6nvwkz4wvmk/v1
Autor:
Sara K Goetz, Herman KH Fung, Sven Klumpe, Ievgeniia Zagoriy, Bernhard Hampoelz, Xiaojie Zhang, Philipp S Erdmann, Janina Baumbach, Christoph W Müller, Martin Beck, Jürgen M Plitzko, Julia Mahamid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::11ccc320717983ed5784e44e30c76b7d
https://doi.org/10.7554/elife.70506.sa2
https://doi.org/10.7554/elife.70506.sa2
Autor:
Sara K Goetz, Herman KH Fung, Sven Klumpe, Ievgeniia Zagoriy, Bernhard Hampoelz, Xiaojie Zhang, Philipp S Erdmann, Janina Baumbach, Christoph W Müller, Martin Beck, Jürgen M Plitzko, Julia Mahamid
Publikováno v:
eLife
eLife, Vol 10 (2021)
elife
eLife, Vol 10 (2021)
elife
Lamella micromachining by focused ion beam milling at cryogenic temperature (cryo-FIB) has matured into a preparation method widely used for cellular cryo-electron tomography. Due to the limited ablation rates of low Ga+ ion beam currents required to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::957649178edea395e1b22cbddd671d81
https://pubmed.ncbi.nlm.nih.gov/34951584
https://pubmed.ncbi.nlm.nih.gov/34951584
Publikováno v:
Science Advances
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=r3c4b2081b22::87856ccf9ffb1051b9a798e087ed70a4