Zobrazeno 1 - 10
of 41
pro vyhledávání: '"Philip Youngman"'
Publikováno v:
Microbiology. 150:321-333
Expression of the major virulence cluster in Listeria monocytogenes is positively regulated by the transcription factor PrfA and is influenced by several environmental factors, including the presence of readily metabolized carbohydrates such as cello
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e6d0f73566a8e966b2038aee9b99414
https://doi.org/10.1099/mic.0.26718-0
https://doi.org/10.1099/mic.0.26718-0
Autor:
Chester W. Price, Hélène Cérémonie, Nancy Su, Philip Youngman, Christopher K. Murphy, Paul Fawcett
Publikováno v:
Molecular Microbiology. 41:757-774
Bacteria respond to diverse growth-limiting stresses by producing a large set of general stress proteins. In Bacillus subtilis and related Gram-positive pathogens, this response is governed by the sigma(B) transcription factor. To establish the range
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d4a118633c116fc518d73796d6657945
https://doi.org/10.1046/j.1365-2958.2001.02534.x
https://doi.org/10.1046/j.1365-2958.2001.02534.x
Autor:
Philip Youngman
Publikováno v:
#N# Bacillus subtilis and Other Gram-Positive Bacteria
This chapter focuses on the Enterococcus faecalis element Tn917, which is the most extensively used transposon in Bacillus subtilis. Numerous derivatives of Tn917 have been developed to facilitate the cloning and functional analysis of regulated gene
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8c91489c8293782e52dbcdf1e15befcc
https://doi.org/10.1128/9781555818388.ch40
https://doi.org/10.1128/9781555818388.ch40
Autor:
Philip Youngman, Janet K. Hatt
Publikováno v:
Journal of Bacteriology. 182:6975-6982
The Spo0A protein of Bacillus subtilis is a DNA-binding protein that is required for the expression of genes involved in the initiation of sporulation. Spo0A binds directly to and both activates and represses transcription from the promoters of sever
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd9772fe317c6065e65e91f8c83bc0af
https://doi.org/10.1128/jb.182.24.6975-6982.2000
https://doi.org/10.1128/jb.182.24.6975-6982.2000
Autor:
Philip Youngman, L. Jeannine Brady, Dennis G. Cvitkovitch, Jeffrey D. Hillman, Paula J. Crowley, James E. Wetz, Arnold S. Bleiweis, Jaideep Behari, Juan A. Gutierrez
Publikováno v:
FEMS Microbiology Letters. 182:149-154
Previously, we demonstrated successful Tn917 mutagenesis of the oral pathogen Streptococcus mutans using pTV1-OK (Km(r), repATs), a temperature conditional replicative delivery vector carrying a lactococcal pWVO1Ts backbone. In this report we describ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::efaa5e0dafa43eaba1e546253494d329
https://doi.org/10.1111/j.1574-6968.2000.tb08889.x
https://doi.org/10.1111/j.1574-6968.2000.tb08889.x
Autor:
Wendy A. Offen, Anthony J. Wilkinson, Philip Youngman, Katarína Muchová, James A. Brannigan, Richard J. Lewis, David Brown, Imrich Barák
Publikováno v:
Acta Crystallographica Section D Biological Crystallography. 55:671-676
The key response-regulator gene of sporulation, spo0A, has been cloned from Bacillus stearothermophilus and the encoded protein purified. The DNA-binding and phospho-acceptor domains of Spo0A have been prepared by tryptic digestion of the intact prot
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6fb61afd1ca1269f912c91e08591e2ee
https://doi.org/10.1107/s0907444998012682
https://doi.org/10.1107/s0907444998012682
Autor:
Alexandre Melnikov, Philip Youngman
Publikováno v:
Nucleic Acids Research. 27:1056-1062
We describe a general method for random mutagenesis of cloned genes by error-prone PCR or DNA shuffling that eliminates the need for post-amplification subcloning following each cycle of mutagenesis. This method exploits the highly efficient and reco
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44a9abced4df4dca9f9ee1043b98a317
https://doi.org/10.1093/nar/27.4.1056
https://doi.org/10.1093/nar/27.4.1056
Publikováno v:
Infection and Immunity. 67:131-139
Listeria monocytogenes requires listeriolysin O (LLO) and ActA, the products of hly and actA , respectively, to establish a productive intracellular infection. LLO is essential for vacuolar lysis and entry into the cytosol, while ActA is required for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11c22ab198bd3c23b53577cac259bedc
https://doi.org/10.1128/iai.67.1.131-139.1999
https://doi.org/10.1128/iai.67.1.131-139.1999
Autor:
Janet K. Hatt, Philip Youngman
Publikováno v:
Journal of Bacteriology. 180:3584-3591
The transcription factor Spo0A of Bacillus subtilis has the unique ability to activate transcription from promoters that require different forms of RNA polymerase holoenzyme. One class of Spo0A-activated promoter, which includes spoIIEp , is recogniz
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47b7e856e8710c2dbdedd34ffb3a82b2
https://doi.org/10.1128/jb.180.14.3584-3591.1998
https://doi.org/10.1128/jb.180.14.3584-3591.1998
Autor:
Philip Youngman, Jaideep Behari
Publikováno v:
Journal of Bacteriology. 180:6316-6324
Readily utilizable sugars down-regulate virulence gene expression in Listeria monocytogenes , which has led to the proposal that this regulation may be an aspect of global catabolite regulation (CR). We recently demonstrated that the metabolic enzyme
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::79d017368be67204c04c1a65e9894338
https://doi.org/10.1128/.180.23.6316-6324.1998
https://doi.org/10.1128/.180.23.6316-6324.1998