Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Philip R. Tempest"'
Autor:
Marie L. Fernie, Premala H. Fawcett, Philip R. Tempest, William J. Harris, Geraldine Taylor, Patricia White, Kathryn L. Armour, Susan I. King
Publikováno v:
Molecular Immunology. 31:1369-1372
Oligodeoxyribonucleotide primers based on the 5' ends of bovine IgG1/2 and lambda constant (C) region genes, together with primers encoding conserved amino acids at the N-terminus of mature variable (V) regions from other species, have been used in c
Autor:
Philip R. Tempest, Elena Barbanti, Marta Ghislieri, Frank J. Carr, Patricia Bremner, Fabrizio Marcucci, Bruno Rifaldi
Publikováno v:
Hybridoma. 13:183-190
We have constructed several humanized versions of a monoclonal antibody (MAb78) against human tumor necrosis factor-alpha (huTNF-alpha) retaining the complementarity-determining regions (CDR) of the original mouse MAb with or without a variable numbe
Publikováno v:
Journal of Molecular Biology. 235:53-60
The contribution of framework regions (FRs) of antibody-variable domains to idiotype expression was studied by examining the interaction of various “humanized” versions of a mouse anti-TNF α monoclonal antibody (mAb78) with polyclonal and two mo
Publikováno v:
Veterinary immunology and immunopathology. 71(3-4)
In order to investigate the role of T cells in immune responses to infectious pathogens, depletion of individual T cell subsets using monoclonal antibodies (mAbs) is commonly undertaken. Since most mAbs are of murine origin, such depletion studies in
Autor:
Ray Field, Philip R. Tempest, Julia Elizabeth Thompson, Andrew James Williams, Tristan J. Vaughan, Ronald Henry Jackson, Mia Martins, Jonathan Alexander Green, Jane Wilton, Kevin Stuart Johnson, Steven Paul Ruddock, Louise Bacon, Anthony Richard Pope
Publikováno v:
Journal of immunological methods. 227(1-2)
Phage display provides a methodology for obtaining fully human antibodies directed against human transforming growth factor-beta (TGFbeta) suitable for the treatment of fibrotic disorders. The strategy employed was to isolate a human single chain Fv
Publikováno v:
Nature biotechnology. 16(6)
Monoclonal antibodies (Mabs) have long been considered a good class of natural drugs, both because they mimic their natural role in the body and because they have no inherent toxicity. Although rodent Mabs are readily generated, their widespread use
Reshaping a human monoclonal antibody to inhibit human respiratory syncytial virus infection in vivo
Autor:
Martin Lambert, Philip R. Tempest, William J. Harris, Geraldine Taylor, J. Furze, Frank J. Carr, Patricia Bremner
Publikováno v:
Bio/technology (Nature Publishing Company). 9(3)
We transferred the complementarity determining regions from a murine monoclonal antibody that neutralizes infection by respiratory syncytial virus (RSV) to a human IgG1 monoclonal antibody. The resulting reshaped human antibody lost affinity for RSV,
Autor:
Philip R. Tempest, B. E. B. Moseley
Publikováno v:
Molecular and General Genetics MGG. 179:191-199
The lethal and mutagenic effects of methyl methanesulphonate (MMS), ethyl methanesulphonate (EMS), and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) can be dissociated in a mitomycin C (MTC)-sensitive mutant, strain 302, of Micrococcus radiodurans. As
Autor:
Philip R. Tempest, B. E. B. Moseley
Publikováno v:
Mutation Research Letters. 104:275-280
Ultraviolet (UV) radiation did not induce rifampicin-resistant mutants in populations of the taxonomically-related radiation-resistant bacteria Deinococcus radiodurans, D. radiopugnans, D. radiophilus and D. proteolyticus, although such mutants arose
Publikováno v:
FEBS letters. 209(2)
We have raised antibodies against a synthetic dodecapeptide corresponding to the carboxyl terminus of the predicted met gene product. Phosphorylation of 60 kDa and 65 kDa proteins on tyrosine residues was observed when immunoprecipitates of cells con