Výsledky vyhledávání - "Philip M. Kao"

Use of maleimide-thiol coupling chemistry for efficient syntheses of oligonucleotide-enzyme conjugate hybridization probes

Autor: Soumitra S. Ghosh, Ann W. McCue, Philip M. Kao, Hugh L. Chappelle

Publikováno v: Bioconjugate chemistry. 1(1)

Two general methods which exploit the reactivity of sulfhydryl groups toward maleimides are described for the synthesis of oligonucleotide-enzyme conjugates for use as nonradioisotopic hybridization probes. In the first approach, 6-maleimidohexanoic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9abfc818f31dab115b9f497282219e3a
https://pubmed.ncbi.nlm.nih.gov/2128871

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Synthesis of 5′-oligonucleotide hydrazide derivatives and their use in preparation of enzyme-nucleic acid hybridization probes

Autor: Deborah Y. Kwoh, Philip M. Kao, Soumitra S. Ghosh

Publikováno v: Analytical Biochemistry. 178:43-51

A hydrazone-based method for conjugating synthetic nucleic acids and reporter molecules for use as nonradioactive hybridization probes is presented. Oligonucleotides complementary to the hepatitis B virus were derivatized at their 5′ ends with hydr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee5be473a7d70d8304105e7c7017634e
https://doi.org/10.1016/0003-2697(89)90354-0

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