Zobrazeno 1 - 2
of 2
pro vyhledávání: '"Phil Marks"'
Publikováno v:
Nucleic Acids Research. 31:2803-2810
We have cloned the M and S genes of the restriction-modification (R-M) system AhdI and have purified the resulting methyltransferase to homogeneity. M.AhdI is found to form a 170 kDa tetrameric enzyme having a subunit stoichiometry M2S2 (where the M
Publikováno v:
Protein expression and purification. 37(1)
Biochemical and structural studies of the methylase from the type 1 1 2 R–M system Ahd I require the ability to purify this multi-subunit enzyme in significant quantities in a soluble and active form. Several Escherichia coli expression systems wer