Targeted mutagenesis of the Arabidopsis GROWTH-REGULATING FACTOR (GRF) gene family suggests competition of multiplexed sgRNAs for Cas9 apoprotein

Autor: Isaack Ke, Kobylanski Js, Bauer O, Bowen Nm, Angulo J, Astin Cp, Pham Rt, Ingram Am, Dinofrio As, Morales Jm, Wolfgang Lukowitz, Vo Hd, Jeong G, Phillips Mh, Chukwudinma Nj, Kiser Rj, Yeary Ka, Blash Kj, Reel Tw, Manning Ga, Gusinde-Duffy Cm, Ghulam Am, Wukuson Am, Faletti Do, Seo Je, Zheng Gy, Horace Kj, Nguyen Kh, Long Mr

Genome editing in plants typically relies on T-DNA plasmids that are mobilized by Agrobacterium-mediated transformation to deliver the CRISPR/Cas9 machinery. Here, we introduce a series of CRISPR/Cas9 T-DNA vectors for minimal lab settings, such as i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f30b15cd4b5232c67f962dd12f2fe17c
https://doi.org/10.1101/2020.08.16.253203

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CRISPR/Cas9 mutagenesis of the Arabidopsis GROWTH-REGULATING FACTOR (GRF) gene family.

Autor: Angulo J; Department of Plant Biology, University of Georgia, Athens, GA, United States., Astin CP; Division of Biology, University of Georgia, Athens, GA, United States., Bauer O; Department of Plant Biology, University of Georgia, Athens, GA, United States., Blash KJ; Division of Biology, University of Georgia, Athens, GA, United States., Bowen NM; Division of Biology, University of Georgia, Athens, GA, United States., Chukwudinma NJ; Division of Biology, University of Georgia, Athens, GA, United States., DiNofrio AS; Division of Biology, University of Georgia, Athens, GA, United States., Faletti DO; Division of Biology, University of Georgia, Athens, GA, United States., Ghulam AM; Division of Biology, University of Georgia, Athens, GA, United States., Gusinde-Duffy CM; Division of Biology, University of Georgia, Athens, GA, United States., Horace KJ; Division of Biology, University of Georgia, Athens, GA, United States., Ingram AM; Division of Biology, University of Georgia, Athens, GA, United States., Isaack KE; Division of Biology, University of Georgia, Athens, GA, United States., Jeong G; Division of Biology, University of Georgia, Athens, GA, United States., Kiser RJ; Division of Biology, University of Georgia, Athens, GA, United States., Kobylanski JS; Department of Plant Biology, University of Georgia, Athens, GA, United States., Long MR; Department of Plant Biology, University of Georgia, Athens, GA, United States., Manning GA; Department of Plant Biology, University of Georgia, Athens, GA, United States., Morales JM; Division of Biology, University of Georgia, Athens, GA, United States., Nguyen KH; Division of Biology, University of Georgia, Athens, GA, United States., Pham RT; Division of Biology, University of Georgia, Athens, GA, United States., Phillips MH; Department of Plant Biology, University of Georgia, Athens, GA, United States., Reel TW; Division of Biology, University of Georgia, Athens, GA, United States., Seo JE; Division of Biology, University of Georgia, Athens, GA, United States., Vo HD; Division of Biology, University of Georgia, Athens, GA, United States., Wukoson AM; Division of Biology, University of Georgia, Athens, GA, United States., Yeary KA; Department of Plant Biology, University of Georgia, Athens, GA, United States., Zheng GY; Department of Plant Biology, University of Georgia, Athens, GA, United States., Lukowitz W; Department of Plant Biology, University of Georgia, Athens, GA, United States.

Publikováno v: Frontiers in genome editing [Front Genome Ed] 2023 Oct 16; Vol. 5, pp. 1251557. Date of Electronic Publication: 2023 Oct 16 (Print Publication: 2023).

Patient Perceptions of Telephonic Visits during the COVID-19 Pandemic-Is Video Connectivity Imperative?

Autor: Luse DC; University of Texas Health Science Center at Houston, McGovern Medical School, Houston, Texas., Palasi SF; University of Texas Health Science Center at Houston, McGovern Medical School, Houston, Texas., Geskin AA; University of Texas Health Science Center at Houston, McGovern Medical School, Houston, Texas., Pham RT; University of Texas Health Science Center at Houston, McGovern Medical School, Houston, Texas., Ayoub HI; University of Texas Health Science Center at Houston, McGovern Medical School, Houston, Texas., Canfield SE; University of Texas Health Science Center at Houston, McGovern Medical School, Houston, Texas.

Publikováno v: Urology practice [Urol Pract] 2021 Jul; Vol. 8 (4), pp. 460-465. Date of Electronic Publication: 2021 Apr 15.