Digital Microfluidics for Single Bacteria Capture and Selective Retrieval Using Optical Tweezers

Autor: Phalguni Tewari Kumar, Deborah Decrop, Saba Safdar, Ioannis Passaris, Tadej Kokalj, Robert Puers, Abram Aertsen, Dragana Spasic, Jeroen Lammertyn

Publikováno v: Micromachines, Vol 11, Iss 3, p 308 (2020)

When screening microbial populations or consortia for interesting cells, their selective retrieval for further study can be of great interest. To this end, traditional fluorescence activated cell sorting (FACS) and optical tweezers (OT) enabled metho

Externí odkaz: https://doaj.org/article/7261beadb7be42c68e8c3f4f99495354

Digital Microfluidics Assisted Sealing of Individual Magnetic Particles in Femtoliter-Sized Reaction Wells for Single-Molecule Detection

Autor: Deborah, Decrop, Elena Pérez, Ruiz, Phalguni Tewari, Kumar, Lisa, Tripodi, Tadej, Kokalj, Jeroen, Lammertyn

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1547

Digital microfluidics has emerged in the last years as a promising liquid handling technology for a variety of applications. Here, we describe in detail how to build up an electrowetting-on-dielectric-based digital microfluidic chip with unique advan

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::8207efbcd28572f17d082cdcbbeffcb7
https://pubmed.ncbi.nlm.nih.gov/28044289

Increasing the fungicidal action of amphotericin B by inhibiting the nitric oxide-dependent tolerance pathway

Autor: Tadej Kokalj, Caroline Struyfs, Paula Ludovico, Karin Thevissen, Pieter Spincemaille, Belém Sampaio-Marques, Kim Vriens, Jeroen Lammertyn, Tanne L. Cools, Bruno P. A. Cammue, Phalguni Tewari Kumar

Publikováno v: Oxidative Medicine and Cellular Longevity, Vol 2017 (2017)
Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
Oxidative Medicine and Cellular Longevity

Amphotericin B (AmB) induces oxidative and nitrosative stresses, characterized by production of reactive oxygen and nitrogen species, in fungi. Yet, how these toxic species contribute to AmB-induced fungal cell death is unclear. We investigated the r

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c65a0768f4dbfec298f658bee4ae2a22
https://lirias.kuleuven.be/handle/123456789/625595

Digital microfluidics for time-resolved cytotoxicity studies on single non-adherent yeast cells

Autor: Bruno Cammue, Robert Puers, Phalguni Tewari Kumar, Kim Vriens, Tadej Kokalj, Matteo Cornaglia, Karin Thevissen, Jeroen Lammertyn, Annemie Geeraerd, Martin A. M. Gijs

Publikováno v: Lab on a chip. 15(8)

Single cell analysis (SCA) has gained increased popularity for elucidating cellular heterogeneity at genomic, proteomic and cellular levels. Flow cytometry is considered as one of the most widely used techniques to characterize single cell responses;

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5d850258a02fa53b43d05a973484be0
https://pubmed.ncbi.nlm.nih.gov/25710603