A cell-based bioluminescence reporter assay of human Sonic Hedgehog protein autoprocessing to identify inhibitors and activators.

Autor: Ciulla DA; Chemistry Department, Binghamton University, Binghamton, New York, USA., Dranchak P; National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland, USA., Pezzullo JL; State University of New York, College of Environmental Science and Forestry, Syracuse, New York, USA., Mancusi RA; Chemistry Department, Binghamton University, Binghamton, New York, USA., Psaras AM; Chemistry Department, Binghamton University, Binghamton, New York, USA., Rai G; National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland, USA., Giner JL; State University of New York, College of Environmental Science and Forestry, Syracuse, New York, USA. Electronic address: jlginer@syr.edu., Inglese J; National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland, USA; National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, USA. Electronic address: jinglese@mail.nih.gov., Callahan BP; Chemistry Department, Binghamton University, Binghamton, New York, USA. Electronic address: callahan@binghamton.edu.

Publikováno v: The Journal of biological chemistry [J Biol Chem] 2022 Dec; Vol. 298 (12), pp. 102705. Date of Electronic Publication: 2022 Nov 16.

Subverting Hedgehog Protein Autoprocessing by Chemical Induction of Paracatalysis.

Autor: Smith CJ; Department of Chemistry , Binghamton University, State University of New York , 4400 Vestal Parkway East , Binghamton , New York 13902 , United States., Wagner AG; Department of Chemistry , Binghamton University, State University of New York , 4400 Vestal Parkway East , Binghamton , New York 13902 , United States., Stagnitta RT; Department of Chemistry , Binghamton University, State University of New York , 4400 Vestal Parkway East , Binghamton , New York 13902 , United States., Xu Z; Department of Chemistry , Binghamton University, State University of New York , 4400 Vestal Parkway East , Binghamton , New York 13902 , United States., Pezzullo JL; Department of Chemistry , State University of New York College of Environmental Science and Forestry , Syracuse , New York 13210 , United States., Giner JL; Department of Chemistry , State University of New York College of Environmental Science and Forestry , Syracuse , New York 13210 , United States., Xie J; Department of Biological Sciences, Center for Biotechnology and Interdisciplinary Studies , Rensselaer Polytechnic Institute , 110 8th Street , Troy , New York 12180 , United States., Covey DF; Department of Developmental Biology , Taylor Family Institute for Innovative Psychiatric Research , 660 South Euclid Avenue , St. Louis , Missouri 63110 , United States., Wang C; Department of Biological Sciences, Center for Biotechnology and Interdisciplinary Studies , Rensselaer Polytechnic Institute , 110 8th Street , Troy , New York 12180 , United States., Callahan BP; Department of Chemistry , Binghamton University, State University of New York , 4400 Vestal Parkway East , Binghamton , New York 13902 , United States.

Publikováno v: Biochemistry [Biochemistry] 2020 Feb 18; Vol. 59 (6), pp. 736-741. Date of Electronic Publication: 2020 Feb 04.