Structures of influenza A and B replication complexes give insight into avian to human host adaptation and reveal a role of ANP32 as an electrostatic chaperone for the apo-polymerase

Autor: Benoît Arragain, Tim Krischuns, Martin Pelosse, Petra Drncova, Martin Blackledge, Nadia Naffakh, Stephen Cusack

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-20 (2024)

Abstract Replication of influenza viral RNA depends on at least two viral polymerases, a parental replicase and an encapsidase, and cellular factor ANP32. ANP32 comprises an LRR domain and a long C-terminal low complexity acidic region (LCAR). Here w

Externí odkaz: https://doaj.org/article/eb9333d58028408b951a16e109240b80

Backtracking of influenza polymerase upon consecutive incorporation of nucleoside analogue T1106 directly observed by high-resolution cryo-electron microscopy

Autor: Tomas Kouba, Anna Dubankova, Petra Drncova, Elisa Donati, Pietro Vidossich, Valentina Speranzini, Alex Pflug, Johanna Huchting, Chris Meier, Marco De Vivo, Stephen Cusack

The broad-spectrum antiviral pseudobase T705, a fluorinated pyrazinecarboxamide, is incorporated via its triphosphate form into nascent viral RNA by viral RNA-dependent RNA polymerases. Since it mimics guanine or adenine it can act as a mutagen, wher

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a76016e11bf89059e30023f8d2682ce6
https://doi.org/10.1101/2022.06.10.495428

Structural snapshots of actively transcribing influenza polymerase

Autor: Tomas Kouba, Stephen Cusack, Petra Drncova

Publikováno v: Nature structural & molecular biology

Influenza virus RNA-dependent RNA polymerase uses unique mechanisms to transcribe its single-stranded genomic viral RNA (vRNA) into messenger RNA. The polymerase is initially bound to a promoter comprising the partially base-paired 3′ and 5′ extr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6ba39b6e4d8f9fcb2499d0b0c2eef45
http://europepmc.org/articles/PMC7610713

High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory™, a MultiBac Baculoviral Genome Customized for Enveloped VLP Expression

Autor: Imre Berger, Magdalena Janina Laska, Daniel J. Fitzgerald, Frederic Garzoni, Petra Drncova, Duygu Sari-Ak, Christiane Schaffitzel, Shervin Bahrami

Publikováno v: Methods in Molecular Biology ISBN: 9781493996230
Sari-Ak, D, Bahrami, S, Laska, M, Drncova, P, Fitzgerald, D J, Berger-Schaffitzel, C, Garzoni, F & Berger, I 2019, High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory™, a MultiBac Baculoviral Genome Customized for Enveloped VLP Expression . in R Vincentelli (ed.), High-Throughput Protein Production and Purification : Methods and Protocols . Methods in Molecular Biology, vol. 2025, Humana Press, pp. 213-226 . https://doi.org/10.1007/978-1-4939-9624-7_10
Sari-Ak, D, Bahrami, S, Laska, M J, Drncova, P, Fitzgerald, D J, Schaffitzel, C, Garzoni, F & Berger, I 2019, High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory™, a MultiBac Baculoviral Genome Customized for Enveloped VLP Expression . in R Vincentelli (ed.), High-Throughput Protein Production and Purification : Methods and Protocols . vol. 2025, Humana Press, Methods in molecular biology (Clifton, N.J.), pp. 213-226 . https://doi.org/10.1007/978-1-4939-9624-7_10

Baculovirus-based expression of proteins in insect cell cultures has emerged as a powerful technology to produce complex protein biologics for many applications ranging from multiprotein complex structural biology to manufacturing of therapeutic prot

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1a5e061533581221fec7f62719f110b
https://doi.org/10.1007/978-1-4939-9624-7_10