Telomerase RNA in Hymenoptera (Insecta) switched to plant/ciliate-like biogenesis

Autor: Petr Fajkus, Matej Adámik, Andrew D L Nelson, Agata M Kilar, Michal Franek, Michal Bubeník, Radmila Čapková Frydrychová, Alena Votavová, Eva Sýkorová, Jiří Fajkus, Vratislav Peška

Publikováno v: Nucleic acids research.

In contrast to the catalytic subunit of telomerase, its RNA subunit (TR) is highly divergent in size, sequence and biogenesis pathways across eukaryotes. Current views on TR evolution assume a common origin of TRs transcribed with RNA polymerase II i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::166c685f9d8fcebb71275e2b92bebf56
https://pubmed.ncbi.nlm.nih.gov/36546771

Terminal Restriction Fragments (TRF) Method to Analyze Telomere Lengths

Autor: Miloslava Fojtová, Petr Fajkus, Pavla Polanská, Jiří Fajkus

Publikováno v: Bio-Protocol, Vol 5, Iss 23 (2015)

Chromosome ends - telomeres - are a focus of intensive research due to their importance for the maintenance of chromosome stability. Their shortening due to incomplete replication functions as a molecular clock counting the number of cell divisions,

Externí odkaz: https://doaj.org/article/a7bc082b80e34b218c2fee78ec8309b3

Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae

Autor: Petr Fajkus, Sònia Garcia, Jiří Fajkus, Vojtěch Dvořáček, Václav Brázda, Natália Bohálová, Michal Bubeník, Vratislav Peška

Publikováno v: Scientific Reports
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Consejo Superior de Investigaciones Científicas (CSIC)
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Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)

Telomerase RNA (TR) carries the template for synthesis of telomere DNA and provides a scaffold for telomerase assembly. Fungal TRs are long and have been compared to higher eukaryotes, where they show considerable diversity within phylogenetically cl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b676ba4b89aaaf76f202872969435085
https://pubmed.ncbi.nlm.nih.gov/34140564

Telomerase RNAs in land plants

Autor: Martina Dvořáčková, Jana Fulnečková, Šimon Dobias, Vratislav Peška, Dagmar Zachová, Agata Magdalena Kilar, Jiří Fajkus, Petr Fajkus, Jason Sims, Ivona Nečasová, Eva Sýkorová, Michal Závodník, Miloslava Fojtová

Publikováno v: Nucleic Acids Research

To elucidate the molecular nature of evolutionary changes of telomeres in the plant order Asparagales, we aimed to characterize telomerase RNA subunits (TRs) in these plants. The unusually long telomere repeat unit in Allium plants (12 nt) allowed us

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6721085e7904fe035e08dd1ca09ceb8

BAL31-NGS approach for identification of telomeres de novo in large genomes

Autor: Zdeňka Sitová, Petr Fajkus, Jiří Fajkus, Vratislav Peška

Publikováno v: Methods (San Diego, Calif.). 114

This article describes a novel method to identify as yet undiscovered telomere sequences, which combines next generation sequencing (NGS) with BAL31 digestion of high molecular weight DNA. The method was applied to two groups of plants: i) dicots, ge

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed6a03211466d38c723e3d70ccb84682
https://pubmed.ncbi.nlm.nih.gov/27595912