Uncoupled evolution of the Polycomb system and deep origin of non-canonical PRC1

Autor: Bastiaan de Potter, Maximilian W. D. Raas, Michael F. Seidl, C. Peter Verrijzer, Berend Snel

Publikováno v: Communications Biology, Vol 6, Iss 1, Pp 1-12 (2023)

Abstract Polycomb group proteins, as part of the Polycomb repressive complexes, are essential in gene repression through chromatin compaction by canonical PRC1, mono-ubiquitylation of histone H2A by non-canonical PRC1 and tri-methylation of histone H

Externí odkaz: https://doaj.org/article/3f3f16f72ef44fc0b2a68f4ba68e0539

In vivo analysis reveals that ATP-hydrolysis couples remodeling to SWI/SNF release from chromatin

Autor: Ben C Tilly, Gillian E Chalkley, Jan A van der Knaap, Yuri M Moshkin, Tsung Wai Kan, Dick HW Dekkers, Jeroen AA Demmers, C Peter Verrijzer

Publikováno v: eLife, Vol 10 (2021)

ATP-dependent chromatin remodelers control the accessibility of genomic DNA through nucleosome mobilization. However, the dynamics of genome exploration by remodelers, and the role of ATP hydrolysis in this process remain unclear. We used live-cell i

Externí odkaz: https://doaj.org/article/9127d0482a2844bc94f9f9006ff6118e

A Testis-Specific Chaperone and the Chromatin Remodeler ISWI Mediate Repackaging of the Paternal Genome

Autor: Cécile M. Doyen, Gillian E. Chalkley, Olaf Voets, Karel Bezstarosti, Jeroen A. Demmers, Yuri M. Moshkin, C. Peter Verrijzer

Publikováno v: Cell Reports, Vol 13, Iss 7, Pp 1310-1318 (2015)

During spermatogenesis, the paternal genome is repackaged into a non-nucleosomal, highly compacted chromatin structure. Bioinformatic analysis revealed that Drosophila sperm chromatin proteins are characterized by a motif related to the high-mobility

Externí odkaz: https://doaj.org/article/2bcc30d7ebaa4c21839a8d152e0be969

USP7 regulates the ncPRC1 Polycomb axis to stimulate genomic H2AK119ub1 deposition uncoupled from H3K27me3

Autor: Ayestha Sijm, Yaser Atlasi, Jan A. van der Knaap, Joyce Wolf van der Meer, Gillian E. Chalkley, Karel Bezstarosti, Dick H. W. Dekkers, Wouter A. S. Doff, Zeliha Ozgur, Wilfred F. J. van IJcken, Jeroen A. A. Demmers, C. Peter Verrijzer

Publikováno v: Science advances, 8(44):abq7598. American Association for the Advancement of Science
Sijm, A, Atlasi, Y, van der Knaap, J A, van der Meer, J W, Chalkley, G E, Bezstarosti, K, Dekkers, D H W, Doff, W A S, Ozgur, Z, van IJcken, W F J, Demmers, J A A & Verrijzer, C P 2022, ' USP7 regulates the ncPRC1 Polycomb axis to stimulate genomic H2AK119ub1 deposition uncoupled from H3K27me3 ', Science Advances, vol. 8, no. 44, abq7598 . https://doi.org/10.1126/sciadv.abq7598

Ubiquitin-specific protease 7 (USP7) has been implicated in cancer progression and neurodevelopment. However, its molecular targets remain poorly characterized. We combined quantitative proteomics, transcriptomics, and epigenomics to define the core

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8154b759994b52753de3c189ce8b3ce3
https://pubmed.ncbi.nlm.nih.gov/36332031

Subunits of the Histone Chaperone CAF1 Also Mediate Assembly of Protamine-Based Chromatin

Autor: Cécile M. Doyen, Yuri M. Moshkin, Gillian E. Chalkley, Karel Bezstarosti, Jeroen A.A. Demmers, Christina Rathke, Renate Renkawitz-Pohl, C. Peter Verrijzer

Publikováno v: Cell Reports, Vol 4, Iss 1, Pp 59-65 (2013)

One of the most dramatic forms of chromatin reorganization occurs during spermatogenesis, when the paternal genome is repackaged from a nucleosomal to a protamine-based structure. We assessed the role of the canonical histone chaperone CAF1 in Drosop

Externí odkaz: https://doaj.org/article/e799152876684e05802904ce97bf4633