Saccharomyces cerevisiae-based platform for rapid production and evaluation of eukaryotic nutrient transporters and transceptors for biochemical studies and crystallography.

Autor: Peter Scharff-Poulsen, Per Amstrup Pedersen

Publikováno v: PLoS ONE, Vol 8, Iss 10, p e76851 (2013)

To produce large quantities of high quality eukaryotic membrane proteins in Saccharomyces cerevisiae, we modified a high-copy vector to express membrane proteins C-terminally-fused to a Tobacco Etch Virus (TEV) protease detachable Green Fluorescent P

Externí odkaz: https://doaj.org/article/df5370a61a6c4eb19a9c18f6ebc65fb6

Genetic Analysis of Signal Generation by the Rgt2 Glucose Sensor of Saccharomyces cerevisiae

Autor: Mark Johnston, Peter Scharff-Poulsen, Hisao Moriya

Publikováno v: Scharff-Poulsen, P, Moriya, H & Johnston, M 2018, ' Genetic Analysis of Signal Generation by the Rgt2 Glucose Sensor of Saccharomyces cerevisiae ', G3: Genes, Genomes, Genetics (Bethesda), vol. 8, no. 8, pp. 2685-2696 . https://doi.org/10.1534/g3.118.200338

The yeast S. cerevisiae senses glucose through Snf3 and Rgt2, transmembrane proteins that generate an intracellular signal in response to glucose that leads to inhibition of the Rgt1 transcriptional repressor and consequently to derepression of HXT g

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e470133c4f558ecc5f878cf33c2036e
https://curis.ku.dk/ws/files/201908831/Genetic_Analysis_of_Signal_Generation_by_the_Rgt2_Glucose_Sensor_of_Saccharomyces_cerevisiae.pdf

High yield purification of full-length functional hERG K+ channels produced in Saccharomyces cerevisiae

Autor: Karen Molbaek, Claus Hélix-Nielsen, Dan A. Klaerke, Peter Scharff-Poulsen, Per Amstrup Pedersen

Publikováno v: Hansen, K M, Scharff-Poulsen, P, Hélix-Nielsen, C, Klærke, D A & Pedersen, P A 2015, ' High yield purification of full-length functional hERG K + channels produced in Saccharomyces cerevisiae ', Microbial Cell Factories, vol. 14, 15 . https://doi.org/10.1186/s12934-015-0193-9
Molbaek, K, Scharff-Poulsen, P, Hélix-Nielsen, C, Klaerke, D A & Pedersen, P A 2015, ' High yield purification of full-length functional hERG K+ channels produced in Saccharomyces cerevisiae ', Microbial Cell Factories, vol. 14, no. 1, 15 . https://doi.org/10.1186/s12934-015-0193-9
Microbial Cell Factories

The hERG potassium channel is essential for repolarization of the cardiac action potential. Due to this vital function, absence of unintended and potentially life-threatening interactions with hERG is required for approval of new drugs. The structure

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a892c99d16f215278be8036e00cfeb3
https://curis.ku.dk/portal/da/publications/high-yield-purification-of-fulllength-functional-herg-k-channels-produced-in-saccharomyces-cerevisiae(0fcfbdd9-c3ac-4c50-bce2-32bc79ca34b4).html

High Yield Expression of the Human Ether-à-Go-Go Related Gene (HERG) in Saccharomyces Cerevisiae

Autor: Per Amstrup Pedersen, Dan A. Klaerke, Peter Scharff-Poulsen, Karen Molbaek

Publikováno v: Biophysical Journal. 106(2)

The human Ether-a-go-go related gene (hERG) encodes a voltage-gated potassium channel and represents the molecular correlate of the IKr current, which is one of the potassium currents involved in the repolarizing of the cardiac action potential. Inhi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eefdd49a7c7c396768704026f048fbd5

Recombinant Production of Human Aquaporin-1 to an Exceptional High Membrane Density in Saccharomyces cerevisiae

Autor: Claus Hélix-Nielsen, Peter Scharff-Poulsen, Per Amstrup Pedersen, Julie Bomholt

Publikováno v: Bomholt, J, Helix Nielsen, C, Scharff-Poulsen, P & Pedersen, P A 2014, ' Recombinant Production of Human Aquaporin-1 to an Exceptional High Membrane Density in Saccharomyces Cerevisiae ', Biophysical Journal, vol. 106, no. 2, Supplement 1, pp. 557A-558A . https://doi.org/10.1016/j.bpj.2013.11.3099
Technical University of Denmark Orbit
PLoS ONE
Bomholt, J, Hélix-Nielsen, C, Scharff-Poulsen, P & Pedersen, P A 2013, ' Recombinant Production of Human Aquaporin-1 to an Exceptional High Membrane Density in Saccharomyces cerevisiae ', P L o S One, vol. 8, no. 2, e56431 . https://doi.org/10.1371/journal.pone.0056431
PLoS ONE, Vol 8, Iss 2, p e56431 (2013)

Due to the lipid nature of cellular membranes preventing transport of most solutes between the cytosol and the extracellular environment as well as from the cytosol to the interior of organelles, cellular homeostasis relies on integral membrane prote

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8de5ed5f6ec576d6b1f3971d5e771e72
https://doi.org/10.1371/journal.pone.0056431