Zobrazeno 1 - 10 of 11 pro vyhledávání: '"Peter R. Banks"'
1
Site-specific fluorescent derivatization and liquid chromatographic–mass spectrometric characterization of Long R3 IGF-I for bioanalytical applications
Autor: Michael D. Harvey, Peter R. Banks
Publikováno v: Journal of Chromatography B. 793:115-125
Recombinant Long R3 IGF-I was derivatized with fluorescein isothiocyanate (FITC) at a single location by careful selection of reaction conditions (i.e. pH, and FITC/protein amino group ratio). High-performance liquid chromatography (LC) and electrosp
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd15bb26237420819efb0ce33b814a1f
https://doi.org/10.1016/s1570-0232(03)00369-6
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3
EFFECT OF MICELLAR STRUCTURE ON MEKC SEPARATION
Autor: Peter R. Banks, Dirk Bandilla
Publikováno v: Journal of Liquid Chromatography & Related Technologies. 24:1929-1934
In order to accomplish the selection of a suitable micelle composition in micellar electrokinetic chromatography (MEKC), and to avoid unnecessary experimental work based on trial and error, an unde...
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::86fa94ff3380747dd191c732fafda769
https://doi.org/10.1081/jlc-100104435
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4
Detection of specific antibodies using immunosubtraction and capillary electrophoresis instrumentation
Autor: Peter R. Banks, Donald M. Paquette
Publikováno v: ELECTROPHORESIS. 22:2391-2397
Solution-phase immunoassays based on capillary electrophoresis (CE) separations have been shown to be rapid and simple to perform. The potential for sample matrix interference and incompatibility with multiplexing conditions for antibody detection wh
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3dd449a9da9d6aa1713f7189067b0bb1
https://doi.org/10.1002/1522-2683(200107)22:12<2391::aid-elps2391>3.0.co
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5
Utilization of the non-covalent fluorescent dye, NanoOrange, as a potential clinical diagnostic tool
Autor: M. D. Harvey, Peter R. Banks, C. D. Skinner, V. Bablekis
Publikováno v: Journal of Chromatography B: Biomedical Sciences and Applications. 754:345-356
The commercially available dye, NanoOrange, has been investigated as a potential tool for clinical diagnostics due to its low cost, ease of use, and ability to detect nanomolar concentrations of protein. Virtually non-fluorescent in dilute aqueous so
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ba9a5fbbd268e44b0f10aa31f5026ed7
https://doi.org/10.1016/s0378-4347(00)00627-7
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6
Fluorescent derivatization for low concentration protein analysis by capillary electrophoresis
Autor: Peter R. Banks
Publikováno v: TrAC Trends in Analytical Chemistry. 17:612-622
Fluorescence derivatization can allow for the low concentration analysis of proteins by capillary electrophoresis. Major problems arising from inefficient chemistry and multiple derivatives must be overcome, however, for the method to be successful.
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8d0ab9dec0b6c898f023c429b3961775
https://doi.org/10.1016/s0165-9936(98)00073-9
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7
Subnanomolar detection limit for sodium dodecyl sulfate — capillary gel electrophoresis using a fluorogenic, noncovalent dye
Autor: Peter R. Banks, Michael D. Harvey, Dirk Bandilla
Publikováno v: Electrophoresis. 19:2169-2174
Picomolar limits of detection are obtained using the noncovalent, fluorogenic dye, Sypro Red. The size separation of four commonly used sodium dodecyl sulfate - capillary gel electrophoresis (SDS-CGE) molecular weight markers with 8% linear polyacryl
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9e090ceb043e548e170d0475f70416c
https://doi.org/10.1002/elps.1150191221
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8
Nanomolar derivatizations with 5-carboxyfluorescein succinimidyl ester for fluorescence detection in capillary electrophoresis
Autor: Peter R. Banks, Shung Kei Lau, Michael J. Little, Fabian Zaccardo
Publikováno v: Journal of Chromatography A. 809:203-210
The ability of 5-carboxyfluorescein succinimidyl ester (CFSE) to derivatize amino acids at very low concentrations has been investigated. CFSE can derivatize nanomolar concentrations of amino acids, which represents a 1000-fold improvement compared t
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2b9dad4be1e1e1ccaca02c95071195d2
https://doi.org/10.1016/s0021-9673(98)00165-4
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9
Single-label fluorescent derivatization of peptides
Autor: Donald M. Paquette, Peter R. Banks, Michael J. Little, Michael D. Harvey
Publikováno v: Analytica Chimica Acta. 339:279-288
A one step method for the attachment of a single fluorescent label to peptides through their N-terminus is described. The method employs fluorescein-5-isothiocyanate as the label of choice and a lower than normal derivatization buffer pH that discrim
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c0477e1320929a0030cce3e9c8f57e50
https://doi.org/10.1016/s0003-2670(96)00488-6
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10
Comparison of Three Common Amine Reactive Fluorescent Probes Used for Conjugation to Biomolecules by Capillary Zone Electrophoresis
Autor: Donald M. Paquette, Peter R. Banks
Publikováno v: Bioconjugate Chemistry. 6:447-458
The conjugation of three amine reactive fluorescent probes, each containing the fluorophore fluorescein but different reactive moieties, was compared using the protein myoglobin and the amino acid L-lysine as reagents. The three different reactive mo
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ca334ff7188f0b04d21a2b51f12e400
https://doi.org/10.1021/bc00034a015
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