Zobrazeno 1 - 10
of 197
pro vyhledávání: '"Peter R Cook"'
Publikováno v:
Microsystems & Nanoengineering, Vol 10, Iss 1, Pp 1-11 (2024)
Abstract Assays mimicking in vitro the concentration gradients triggering biological responses like those involved in fighting infections and blood clotting are essential for biomedical research. Microfluidic assays prove especially attractive as the
Externí odkaz:
https://doaj.org/article/a0a021a8710745aca42e44b1d0b793d0
Autor:
Sabyasachi Baboo, Bhaskar Bhushan, Haibo Jiang, Chris R M Grovenor, Philippe Pierre, Benjamin G Davis, Peter R Cook
Publikováno v:
PLoS ONE, Vol 9, Iss 6, p e99346 (2014)
In bacteria, protein synthesis can be coupled to transcription, but in eukaryotes it is believed to occur solely in the cytoplasm. Using pulses as short as 5 s, we find that three analogues--L-azidohomoalanine, puromycin (detected after attaching flu
Externí odkaz:
https://doaj.org/article/28e30f1382274298b23df6b379d8be61
Publikováno v:
PLoS ONE, Vol 7, Iss 7, p e40207 (2012)
Many nucleic acid polymerases function in clusters known as factories. We investigate whether the RNA polymerase (RNAP) of phage T7 also clusters when active. Using 'pulldowns' and fluorescence correlation spectroscopy we find that elongation complex
Externí odkaz:
https://doaj.org/article/cbaf49a517084ceeb0d9a9858fc88d4e
Autor:
Argyris Papantonis, Joshua D Larkin, Youichiro Wada, Yoshihiro Ohta, Sigeo Ihara, Tatsuhiko Kodama, Peter R Cook
Publikováno v:
PLoS Biology, Vol 8, Iss 7, p e1000419 (2010)
It is widely assumed that active RNA polymerases track along their templates to produce a transcript. We test this using chromosome conformation capture and human genes switched on rapidly and synchronously by tumour necrosis factor alpha (TNFalpha);
Externí odkaz:
https://doaj.org/article/0044c955f97f4272a5405fc14a93300e
Publikováno v:
Biology Open. 12
Organoids grow in vitro to reproduce structures and functions of corresponding organs in vivo. As diffusion delivers nutrients over only ∼200 µm, refreshing flows through organoids are required to avoid necrosis at their cores; achieving this is a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2065e888ffda06ed62da119653db1836
https://doi.org/10.1242/bio.059825
https://doi.org/10.1242/bio.059825
Autor:
Cyril Deroy, James H. R. Wheeler, Agata N. Rumianek, Peter R. Cook, William M. Durham, Kevin R. Foster, Edmond J. Walsh
Publikováno v:
ACS Applied Materials & Interfaces. 14:25209-25219
Microfluidic devices are widely used in many fields of biology, but a key limitation is that cells are typically surrounded by solid walls, making it hard to access those that exhibit a specific phenotype for further study. Here, we provide a general
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9316086ebebec76d270e9c0942b3c196
https://doi.org/10.1021/acsami.2c07177
https://doi.org/10.1021/acsami.2c07177
Autor:
Semeraro Massimiliano, Negro Giuseppe, Suma Antonio, Gonnella Giuseppe, Peter R. Cook, Marenduzzo Davide
Within each human cell, different kinds of RNA polymerases and a panoply of transcription factors bind chromatin to simultaneously determine 3D chromosome structure and transcriptional programme. Experiments show that, in some cases, different protei
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a42cf217c844d34036989f1641246ba8
SUMMARYEukaryotic chromosomes compact during mitosis and meiosis into elongated cylinders – and not the spherical globules expected of self-attracting long flexible polymers. This process is mainly driven by condensin-like proteins. Here, we presen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::48fdd3187d308c025941588135927ad0
https://doi.org/10.1101/2022.09.27.509716
https://doi.org/10.1101/2022.09.27.509716
Autor:
Peter R. Cook, Davide Marenduzzo
Publikováno v:
SSRN Electronic Journal.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::45dae10b42dd3662ce3efa595df4270d
https://doi.org/10.2139/ssrn.4178531
https://doi.org/10.2139/ssrn.4178531
Autor:
Peter R. Cook, Cyril Deroy, Cristian Soitu, Edmond J. Walsh, Nicholas Stovall-Kurtz, Federico Nebuloni
Publikováno v:
Microsystems & Nanoengineering
Microsystems & Nanoengineering, Vol 7, Iss 1, Pp 1-9 (2021)
Microsystems & Nanoengineering, Vol 7, Iss 1, Pp 1-9 (2021)
The aqueous phase in traditional microfluidics is usually confined by solid walls; flows through such systems are often predicted accurately. As solid walls limit access, open systems are being developed in which the aqueous phase is partly bounded b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dac3f03c3106adb22550ab8dac8ca089
https://doi.org/10.1038/s41378-021-00322-6
https://doi.org/10.1038/s41378-021-00322-6