Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Peter O. Olins"'
Autor:
Jeng-Jong Shieh, Peter O. Olins, Maire Helena Caparon, William F. Hood, Kumnan Paik, Ann M. Donnelly, Bauer Christopher S, John P. McKearn, Sarah R. Braford, Polazzi Joseph O, John W. Thomas, Jon A. Klover, Alan Michael Easton, David L. Zeng, Mark Allen Abrams, Joseph K. Welply, Barbara K. Klein
Publikováno v:
Experimental Hematology. 27:1746-1756
A combinatorial mutagenesis strategy was used to create a collection of nearly 500 variants of human interleukin 3 (IL-3), each with four to nine amino acid substitutions clustered within four linear, nonoverlapping regions of the polypeptide. The va
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad4c1a8be21dd8999107c60b154356b0
https://doi.org/10.1016/s0301-472x(99)00118-6
https://doi.org/10.1016/s0301-472x(99)00118-6
Publikováno v:
Current Opinion in Biotechnology. 7:494-499
Escherichia coli has long been the primary prokaryotic host for the synthesis of heterologous proteins. Recent advances have been made in the expression of complex proteins as soluble, functional molecules, complete with prosthetic groups, disulfide
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4d3fc527f7c5f74688ed31da724fb83
https://doi.org/10.1016/s0958-1669(96)80051-6
https://doi.org/10.1016/s0958-1669(96)80051-6
Publikováno v:
Molecular Diversity. 1:241-246
Streptavidin-binding peptides containing the consensus amino acid sequence motif EPDW were identified using a phage display library. Phage presenting peptides containing these sequences bound streptavidin in a biotin-sensitive fashion and could be el
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9589642dcccdeb6bc9b84504f50c3ab2
https://doi.org/10.1007/bf01715528
https://doi.org/10.1007/bf01715528
Autor:
William J. Salsgiver, Sharon A. Berberich, George Irvin Glover, Shaukat H. Rangwala, Christine E. Smith, Peter O. Olins, Rory F. Finn, William C. Stallings
Publikováno v:
Gene. 122:263-269
High levels of active HIV-1 protease (PR) were produced in Escherichia coli, amounting to 8–10% of total cell protein. High production levels were achieved by altering the following parameters: (1) codon preference of the coding region, (2) A + T-r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2058c7c2de7fba4ccca37f1c5d327df0
https://doi.org/10.1016/0378-1119(92)90214-a
https://doi.org/10.1016/0378-1119(92)90214-a
Autor:
Peter O. Olins, S C Lee
Publikováno v:
Journal of Biological Chemistry. 267:2849-2852
The effect of overexpression of the heat shock chaperone genes dnaK and groESL on heterologous protein production in Escherichia coli was examined, using a set of related human procollagenase proteins. A diverse range of effects on protein solubility
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cf55ff58d3344fb54c97e3e957f300c8
https://doi.org/10.1016/s0021-9258(19)50659-7
https://doi.org/10.1016/s0021-9258(19)50659-7
Publikováno v:
Nature Biotechnology. 9:477-479
A broad host-range expression plasmid was constructed comprising the incQ replicon, the recA promoter from Escherichia coli and the g10-L ribosome binding site (RBS) derived from bacteriophage T7. The structural genes for porcine somatotropin (pst) a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8323704ac3f4f4bb3b7309d6316624ee
https://doi.org/10.1038/nbt0591-477
https://doi.org/10.1038/nbt0591-477
Autor:
Kumnan Paik, Kris Sterbenz, Barrett R. Thiele, Sarah Ruth Braford-Goldberg, John P. McKearn, Barbara Kure Klein, S. Christopher Bauer, Alan Michael Easton, Peter O. Olins, Joseph O. Polazzi, Jon A. Klover, Maire Helena Caparon
Publikováno v:
The Journal of biological chemistry. 270(40)
A deletion variant of human interleukin-3, hIL-3(15-125), was produced in the periplasmic space of Escherichia coli and had full activity in an AML193.1.3 cell proliferation assay. Libraries of random single-amino acid substitutions were constructed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cad04817236bc23faf3e9c2e7c961cb
https://pubmed.ncbi.nlm.nih.gov/7559548
https://pubmed.ncbi.nlm.nih.gov/7559548
Publikováno v:
Gene. 160(2)
Site-specific transposition in Escherichia coli was used to introduce foreign genes into the Autographica californica nuclear polyhedrosis baculovirus genome. Using a temperature-sensitive donor plasmid and an E. coli host strain with an occupied Tn7
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a8875328f90087e26a39378f36b2961
https://pubmed.ncbi.nlm.nih.gov/7642094
https://pubmed.ncbi.nlm.nih.gov/7642094
Autor:
Brian K. Matthews, Peter O. Olins, Alan D. Levine, Bruce F. Bishop, Michelle A. Peel, Nancy A. Horn, Julia A. Manning, Richard M. Leimgruber, Shaukat H. Rangwala
Publikováno v:
The Journal of biological chemistry. 270(13)
Mouse Interleukin 4 is a 20-kDa glycoprotein, synthesized by activated T lymphocytes and mast cells, which regulates the growth and/or differentiation of a broad spectrum of target cells of the immune system, including B and T lymphocytes, macrophage
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3dd327b28245170828be846d37b728b6
https://pubmed.ncbi.nlm.nih.gov/7706290
https://pubmed.ncbi.nlm.nih.gov/7706290
Autor:
Stephen C. Lee, Peter O. Olins
Publikováno v:
Current opinion in biotechnology. 4(5)
Recent advances in protein expression in E. coli have focused primarily on the enhancement of protein quality. Problems in mRNA translation such as inefficient initiation, mistranslation, frame-shifting and frame-hopping can often be addressed by alt
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24b297765f1c3d4873e7e8b883a66d64
https://pubmed.ncbi.nlm.nih.gov/7764200
https://pubmed.ncbi.nlm.nih.gov/7764200