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Autor:
Auke Bult, J. Leon, E. Reubsaet, Willy J. M. Underberg, Arjan J. Hoekstra, Jan Teeuwsen, Jos H. Beijnen, Peter J.H.J. van Os, Wout P. van Bennekom, Ed Hop
Publikováno v:
Journal of pharmaceutical and biomedical analysis. 19(6)
The Cys36-Cys42 and Cys64-Cys74 disulfide bonds in recombinant methionyl human granulocyte colony-stimulating factor were reduced to sulfhydryls with dithiothreitol or mercury. Both reduction reactions are dependent on the pH. The reduction reaction